Gene Expression


Targeted RNA Sequencing Approaches 1

There are several commercial methods for looking at 10’s or 100’s of gene expression levels via a high throughput TaqMan™ assay from Life Technologies / Thermo Fisher Scientific, a competitive offering from Roche, Douglas Scientific, or also Fluidigm. The limitation of these technologies however is the amount of multiplexing a […]

Happiness is getting more of what you want, in RNA-Seq as in other things...

The Fluidigm C1™ Single Cell Auto Prep System 1

Every human being came from a single cell. While that fact may not be so obvious in our day-to-day routine, the power of a single cell is observed with the burst of research activity in stem cells and induced pluripotent stem cells (and also see the Nobel Prize winner for […]

The Fluidigm C1 IFC chip, borrowed from Fluidigm's Spec Sheet

Biopsy of skin sample courtesy of euthman via Flickr

Single molecule in-situ RNA startup Advanced Cell Diagnostics

FFPE (Formalin-fixed paraffin embedded) tissues are standard preparations in the clinical world. It has been estimated that there are millions of FFPE samples stored in countless hospital laboratories and research pathology groups, all part of a standard operating procedure among pathologists and other hospital personnel. These tissue samples are embedded […]


Readlengths do matter in Next Generation Sequencing 5

Recently I was asked about how important readlengths are, in the context of where MiSeq and Ion Torrent PGM currently stand in the marketplace. As the 454 advertisement used to say until recently, ‘Length Matters’. Given a number of recent announcements from Ion Torrent and the other folks in San […]

Notebook cover from AGBT 2009 (c) Dale Yuzuki