Next Generation Technologist

Next Generation Sequencing, Marketing, and the Genomic Revolution

July 8, 2012
by Dale Yuzuki
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Single Molecule Sequencing – Pacific Biosciences and their method

In previous posts I covered the basics of next-generation sequencing – library preparation, template preparation, and the sequencing methodology itself, whether by pyrophosphate detection, single base extension with reversible terminators, or probe addition by ligation. And single molecule sequencing’s attractiveness … Continue reading

July 5, 2012
by Dale Yuzuki
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Error, alignment, and the myth of the complete genome

The myth of the complete genome is something that is not commonly known to active observers of genomic technologies. (The term ‘active observer’ is from the point of view of one with varying degrees of background in the biological sciences, … Continue reading

July 2, 2012
by Dale Yuzuki
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Oxford Nanopore, the first nanopore-based sequencing technology

Oxford Nanopore, based in Oxford U.K., made a remarkable announcement that surprised many in February’s AGBT meeting in Marco Island. A GridION and MiniION single-molecule sequencers were announced, promising 15 minute runtimes, no sample preparation, and a disposable USB-stick sequencer … Continue reading

May 14, 2012
by Dale Yuzuki
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Some thoughts on Pacific Biosciences single-molecule sequencing

We’re living through a time this year of market transition. The Illumina HiSeq continues to have a strong market position (although the upcoming Ion Torrent Proton sets out to change that, however it won’t be available until this September / … Continue reading

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