Image of Pacific Biosciences' sequencing data, courtesy of a {a href=""}PacBio{/a} investor presentation.
In previous posts I covered the basics of next-generation sequencing – library preparation, template preparation, and the sequencing methodology itself, whether by pyrophosphate detection, single base extension with reversible terminators, or probe addition by ligation. And single molecule sequencing’s attractiveness as a technology has been covered here, but here I’ll […]

Single Molecule Sequencing – Pacific Biosciences and their method

Centaur by {a href=""}JustMN{/a} via Flickr.
The myth of the complete genome is something that is not commonly known to active observers of genomic technologies. (The term ‘active observer’ is from the point of view of one with varying degrees of background in the biological sciences, and is in noway an aspersion.) The ‘first draft’ of […]

Error, alignment, and the myth of the complete genome

Image from Oxford Nanopore
Oxford Nanopore, based in Oxford U.K., made a remarkable announcement that surprised many in February’s AGBT meeting in Marco Island. A GridION and MiniION single-molecule sequencers were announced, promising 15 minute runtimes, no sample preparation, and a disposable USB-stick sequencer for $900 (in the case of the MiniION), with 50kb […]

Oxford Nanopore, the first nanopore-based sequencing technology

SMRTcell of a PacBio RS system courtesy of {a href=""}konradforrstner
We’re living through a time this year of market transition. The Illumina HiSeq continues to have a strong market position (although the upcoming Ion Torrent Proton sets out to change that, however it won’t be available until this September / October). For the time being, customers with NGS platforms are […]

Some thoughts on Pacific Biosciences single-molecule sequencing

The Internet Splat Map, courtesy of {a href=""}jurvetson{/a} via Flickr.
I like hearing from recruiters, and can say that not only because they are salespeople and I am a salesperson at heart. They can be a critical source of difficult to find information, such as their ‘people network’ as well as which employer is looking for which type of talent. […]

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