next-generation sequencing


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Complete Genomics and the Whole Genome Sequencing market 3

Complete Genomics is a startup business founded upon a particular idea – that the whole genome sequencing of human individuals is going to be industrialized, commonplace, and have such clinical utility so as to become the dominant application for next-generation sequencing. (Disclosure – I have no financial interest in this […]


Next Generation Sequencing – Sequencing by Pyrophosphate Release

After preparation of the library (and careful quantitation) and preparation of the amplified template comes the main event: the sequencing itself. While there are several methods available, the methods can be divided into three broad divisions. The three divisions are (firstly) Pyrophosphate Release (named for the original patent by Mostafa […]

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Next Generation Sequencing – Template Preparation 1

After a library is properly prepared, (remember it can be from many sources – randomly sheared genomic DNA, cDNA from a small RNA sample, an immunoprecipitated sample) the library molecules need to be amplified in some manner, before the sequencing takes place. Thus there is a critical need for accurate […]


Next-Generation Sequencing – its historical context

Even though the history of next-generation sequencing is short (the 454 GS20 came out in 2005, the Solexa 1G in 2007, and the SOLiD 2 in 2008), there is a robust genomic revolution going on, and a fierce battle in the marketplace with plummeting costs and soaring throughput. Whether Moore’s […]

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The whole-exome vs. whole-genome sequencing debate

An enterprising salesperson from Complete Genomics used this newfangled social media thing called LinkedIn to make her mark on the world (perhaps) by posing a discussion question. (It was over at the ‘Genome Interpretation‘ group in case you were wondering.) Entitled, “The last days of exome sequencing“, she posed the […]