Next Generation Sequencing – Sequencing by Pyrophosphate Release

After preparation of the library (and careful quantitation) and preparation of the amplified template comes the main event: the sequencing itself. While there are several methods available, the methods can be divided into three broad divisions. The three divisions are (firstly) Pyrophosphate Release (named for the original patent by Mostafa […]

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Next Generation Sequencing – Template Preparation 1

After a library is properly prepared, (remember it can be from many sources – randomly sheared genomic DNA, cDNA from a small RNA sample, an immunoprecipitated sample) the library molecules need to be amplified in some manner, before the sequencing takes place. Thus there is a critical need for accurate […]