Edwin Cuppen (Univ Med Ctr Utrecht) Tissue-specific mutation accumulation in human adult stem cells during life #AGBT17
3:31pm February 14th 2017 via Hootsuite
@Invitae Steve Lincoln presenting a poster with some @SeraCare reference materials #AGBT17 https://t.co/4wvHABqP0W
2:18pm February 14th 2017 via Hootsuite
RT @nebiolabs: Wondering where everyone at #agbt17 is getting these awesome t-shirts? Visit Suite 216 and find out! https://t.co/3lt3X4Oyd7
1:05pm February 14th 2017 via Hootsuite
Blaesius: Goes onto describe their Cellular Research Precise Assays, avoiding PCR bias with molecular indexing #AGBT17
12:40pm February 14th 2017 via Hootsuite
Blaesius: Closes with FACSMelody, 9-color, 3 lasers. scRNA-Seq - capture on size, phenotype, discriminate live/dead cells #AGBT17
12:38pm February 14th 2017 via Hootsuite
Blaesius: Shows experiment with bulk, dissociated, and dissociated and sorted and comparing RNAseq data #AGBT17
12:34pm February 14th 2017 via Hootsuite
Blaesius: Does sample handing affect gene expression? '15 ref (microarray data) https://t.co/UGQ0mR3pSC #AGBT17
12:33pm February 14th 2017 via Hootsuite
Blaesius: Cp RNA seq heatmaps of TCGA and their sorted cells; distinct phenotype maps in sorted cell subpopulations #AGBT17
12:29pm February 14th 2017 via Hootsuite
Blaesius: NGS of sorted cells at 98% purity; used CD184 as a br ca marker for cancer stem cells, RNASeq had clear differentiation #AGBT17
12:27pm February 14th 2017 via Hootsuite
Blaesius: Within mouse, consistent marker quantitation; across PDXs, wide variation. PDX from br ca, shows relatedness #AGBT17
12:22pm February 14th 2017 via Hootsuite
Blaesius: Their PDXs, can look at 10 human-spec markers (CD90, CD194 etc) across 13 PDX individuals #AGBT17
12:21pm February 14th 2017 via Hootsuite
Blaesius: Going from Pt-derived Xenografts (PDX), using tumor-dissociation reagents, with filtration, washing, RBC removal, FACS #AGBT17
12:19pm February 14th 2017 via Hootsuite
At the @BDBiosciences #AGBT17 workshop Rainer Blaesius (R&D, BD) Single-cell Proteogenomics integrating cell sorting and sequencing
12:18pm February 14th 2017 via Hootsuite
This billionaire's cancer moonshot is falling far short of the hype | StatNews https://t.co/qaP1Fkahfi Update on Soon-Shiong’s effort
12:10pm February 14th 2017 via Hootsuite
Colwell: Hospital biofilms in sink drains - tracked to bacteria at strain-level, showed antibiotic resist transmission via plasmids #AGBT17
11:40am February 14th 2017 via Hootsuite
Colwell: Four strains ID'd, only one strain migrated to internal organs. Strains interacted, produced Pseudomonas-like toxin #AGBT17
11:39am February 14th 2017 via Hootsuite
Colwell: Necrotizing fascitis from a zip-line incident '16 Cell https://t.co/lRmbCNBJce Both legs, arms needed amputation (!) #AGBT17
11:38am February 14th 2017 via Hootsuite
Colwell: Can monitor antibiotic resistance genes; also can look at gene abundance related to carbohydrate metabolism #AGBT17
11:35am February 14th 2017 via Hootsuite
Colwell: History: infectious disease and microbial ecology. Cholera affects 7M people/year. 7th pandemic started in '60's #AGBT17
Colwell: Probiotic bacteria likely came from yogurt, buttermilk in diet. A bioforensic tool is the healthy metabiota of the gut #AGBT17
11:34am February 14th 2017 via Hootsuite
Colwell: In the healthy controls: much larger numbers of pathogens. These would make us ill. Colored area in pie chart:protective #AGBT17
11:33am February 14th 2017 via Hootsuite
Colwell: Between 4 and 10 pathogens were present in stool; 50% had Giardia. Unknown etiology: mixture of enteropathic E.coli #AGBT17
11:32am February 14th 2017 via Hootsuite
Colwell: They test for 9 bacteria, 4 parasites, 5 viruses (from Vibrio to Campylobacter, to Cryptosporidium etc) #AGBT17
11:31am February 14th 2017 via Hootsuite
Colwell: Working with a center in Calcutta, provided 74 NICED samples, healthy controls, sick with known and unknown etiology #AGBT17
11:30am February 14th 2017 via Hootsuite
Colwell: Culture diagnostics - time-consuming, now can use MAb's, M-S. to dx in minutes #AGBT17
11:29am February 14th 2017 via Hootsuite
Colwell: Bengal delta is known as 'native homeland' of cholera. Exist naturally; 'highly unlikely cholera will be eradicated' #AGBT17
11:27am February 14th 2017 via Hootsuite
Rita Colwell (Univ MD, CosmosID MD) Metagenomics of hospitalized diarrheal patients and healthy individuals in Kolkata, India #AGBT17
11:24am February 14th 2017 via Hootsuite
Q:Animal-to-human, can that be followed? Rubin: We don't have this data, and are starting to generate it. #AGBT17
11:22am February 14th 2017 via Hootsuite
Rubin: John Snow (influenza, 1918) would consider today's world to be dangerous; 'lots of kindling'. #AGBT17
11:21am February 14th 2017 via Hootsuite
Rubin: Like a wildfire - quicker to detect and respond is critical, to prevent widespread impact. #AGBT17
11:18am February 14th 2017 via Hootsuite
Rubin: Idea is to make vaccines against core proteins against bad viruses, but need in-country capabilities, surveillance. #AGBT17
11:17am February 14th 2017 via Hootsuite
Ruby: Why do this $1B, ten-year project? To convert virology into a data-rich field. 'It is now a mom-and-pop industry' #AGBT17
11:16am February 14th 2017 via Hootsuite
Thanks @SwiftBioSci for the #agbt17 refreshments! (The shirts sure are popular.) https://t.co/PEg3gQ2A1w
Rubin: Describes Predict project, 14 viral families, discovered 1K viruses; 80% novel (Some info: https://t.co/UW42SLh2Fc ) #AGBT17
11:13am February 14th 2017 via Hootsuite
Eddy Rubin (Metabiota CA) The global virome project #AGBT17
11:11am February 14th 2017 via Hootsuite
Cool Lego figurines at #AGBT17 @PacBio suite 317. NB - Sequel is not to scale. https://t.co/SHZXW9a1Ej
10:35am February 14th 2017 via Hootsuite
Q:Panels or WGS or WES? Jones: Didn't show the data behind their choice, but they wanted to build a cohort to ask the Q's #AGBT17
10:28am February 14th 2017 via Hootsuite
Jones: Pt given first-line br ca Rx. Showed BRCA signature, positioned for cisplatin Rx. #AGBT17
10:27am February 14th 2017 via Hootsuite
Jones: Stg IV Lung ca, treated w/carbotaxol 4 cycles, re-diagnosis as extra-mammary breast cancer, Her+, APOBEC sig, ERBB2 amp #AGBT17
10:22am February 14th 2017 via Hootsuite
Jones: Leverages public sources, shares their findings via WashU's CIViC https://t.co/uehYtZQk6x #AGBT17
10:15am February 14th 2017 via Hootsuite
Jones: 17d sample prep, 6d TAT for NGS. Complex pipeline diagrammed. 'All of that in green was custom-developed for POG' LOG, sig's #AGBT17
10:14am February 14th 2017 via Hootsuite
Jones: Able to publish on rare cancer types due to this kind of analyses. Shows day 1 to 17 sample prep workflow thru path rev #AGBT17
10:12am February 14th 2017 via Hootsuite
Jones: Genes downstream of AP-1 are upregulated. Some evidence blood pressure med ibesartan '16 ref https://t.co/cNYErUbEGS #AGBT17
10:10am February 14th 2017 via Hootsuite
Jones: MLH1 del BRAF wt (IHC), enrolled 9/14 (before checkpt inh.) Transcriptome showed FOS-JUN AP1 complex overexpressed #AGBT17
10:08am February 14th 2017 via Hootsuite
Jones: 5x HiSeqX, 4x 2500's, 2x NextSeq. 524 sequenced to-date; 509 analyzed (41 pediatric). One case: CR Stg III, chemo AE #AGBT17
10:07am February 14th 2017 via Hootsuite
Jones: Accessible cohorts of fresh-frozen samples, detailed annotation, most from primary untreated, serial samples are rare #AGBT17
10:05am February 14th 2017 via Hootsuite
Jones: In Canada, est 25K new cases in '14, >10K metastatic. Integrating WGS/RNASeq to affect cancer care #AGBT17
Martin Jones (British Columbia Cancer Agency CAN) Taking whole genomics to the cancer clinic #AGBT17
10:02am February 14th 2017 via Hootsuite
RT @shgoodwin1: OP - path of zika to us. Brazil to Caribbean to Florida. Cruise ship tourism likely route of transmission. #agbt17
10:01am February 14th 2017 via Hootsuite
Carvalho: Claims it is cost-efficient, 'suitable for early detection'. #AGBT17
9:24am February 14th 2017 via Hootsuite
