Mills: Did work on RP protein array; not optimal for the clinic. Attracted to NanoString - broadened from protein, to DNA/RNA #AACR17
10:10am April 3rd 2017 via Hootsuite
Gordon Mills (NanoString workshop) Powering precision oncology w/3D biology technology; intro by Joe Beecham #AACR17
10:09am April 3rd 2017 via Hootsuite
Zitvogel: Onto FMT in germ-free mice: more unpublished data (won't post). ('15 Science https://t.co/bGkekHTkzg ) #AACR17
8:58am April 3rd 2017 via Hootsuite
Zitvogel: As part of Meta-HIT, looked at NSCLC and mRCC. Shows unpublished data; groups up- and down-regulated. #AACR17
8:55am April 3rd 2017 via Hootsuite
Zitvogel: '15 Science https://t.co/oXgW52Tegh Commensal Bifidobacterium helping facilitate anti-PD-L1 activity #AACR17
8:52am April 3rd 2017 via Hootsuite
Zitvogel: Could minimalist microbiome normalize the cancer-immune set point? Synergy bet tumor-targeted and immune-targeted methods #AACR17
8:50am April 3rd 2017 via Hootsuite
Zitvogel: Gut immune system controlling inflamm regions at distant site. Exert functions - called dysbiosis. https://t.co/aXerWGDbig #AACR17
8:49am April 3rd 2017 via Hootsuite
Zitvogel: Cancer-immunity cycle '13 Chen figure https://t.co/l5SUhBDh40 Describes 'cold' vs 'hot' tumors https://t.co/5esvSNeD7i #AACR17
8:48am April 3rd 2017 via Hootsuite
Laurence Zitvogel: A new era of immuno-oncology: anticancer probiotics #AACR17
8:45am April 3rd 2017 via Hootsuite
Allison: Can see CTLA-4 depletes Treg; increase CD4 effector; pockets of CD8 increase. Treg freq correlates w/tumor growth #AACR17
8:39am April 3rd 2017 via Hootsuite
Allison: Uses 43-parameter CyTOF (Fluidigm DVS tech), +/- checkpoint blockade, to ID cells impt for anti-tumor effect #AACR17
8:34am April 3rd 2017 via Hootsuite
Allison: What has been lost - how different aCTLA-4 is to aPD1: hard-wired; CD28; priming; clonal diversity; affects CD4 T-cells #AACR17
8:33am April 3rd 2017 via Hootsuite
Allison: Critical issues - det cellular and molecular mechanisms involved in anti-tumor effect; combine best std-of-care w/agents #AACR17
8:32am April 3rd 2017 via Hootsuite
Allison: 60% surv after 2y (ASCO2016); good % may be alive after 10y (given prior K-M data). #AACR17
8:31am April 3rd 2017 via Hootsuite
Allison: Shows results from PD-L1 NEJM '12 https://t.co/ZPeLurKcsY More recent work is combining aCTLA-4 and aPD-1 65% clin activity #AACR17
8:30am April 3rd 2017 via Hootsuite
Allison: 3y K-M flattens out - 20-22% alive after 10y. Why not higher? Showed PD-L1 induced by gamma-interferon #AACR17
8:29am April 3rd 2017 via Hootsuite
Allison: Showed 10y later, lung tumor free (16y later, single injection). Shows K-M NEJM '10 https://t.co/1mWCnwIvn2 #AACR17
8:27am April 3rd 2017 via Hootsuite
Allison: Many adverse advents are inflammation, controlled by steroids. Longest survivor - showed May '01 after IL-2 progression #AACR17
8:26am April 3rd 2017 via Hootsuite
Allison: Medarex made first human antibody to CTLA-4 (ipilimumab) >70K pts treated to-date; objective response in many tumor types #AACR1
8:25am April 3rd 2017 via Hootsuite
Allison: T-cells proliferate; tumor cells have plenty of antigen, CTLA-4 accumulates to stop the T-cells. #AACR17
8:24am April 3rd 2017 via Hootsuite
Allison: Immune checkpoint blockade - not targeting cancer cells; no cytokines to turn 'on' immune; just released activity #AACR17
8:21am April 3rd 2017 via Hootsuite
Allison: From Ipilumumab 2011, took 3 years; 2 in '14; 5 in '15; 5 in '16; 3 in '17 so far #AACR17
8:20am April 3rd 2017 via Hootsuite
James Allison (MD Anderson TX) Immune checkpoint blockade in cancer therapy: insights, opportunities and prospects for ucres #AACR17
8:18am April 3rd 2017 via Hootsuite
MT @cancerassassin1: Yesterday's #AACR17 plenary talk by Dr Vogelstein is freely available to watch online: https://t.co/7ebdvGFoKK
8:11am April 3rd 2017 via Hootsuite
Contag: Machine learning used to improve as they go. Data storage a challenge. At some point they have to erase it. #AACR17
7:50am April 3rd 2017 via Hootsuite
Q: Data storage, processing? Contag: Stitching is software by military/police for robotic data collection of buildings #AACR17
7:49am April 3rd 2017 via Hootsuite
Q: How to control for movement? Contag: Can pull a vacuum to hold still, prefer to make the optics, system faster #AACR17
7:47am April 3rd 2017 via Hootsuite
Contag: Disclosure - company 'PixelGear' Long list of acknowledgements. #AACR17
7:45am April 3rd 2017 via Hootsuite
Contag: Staining - the goal is to 'get rid of glass slides' to guide the pathologist. Can overlay molecular markers #AACR17
7:44am April 3rd 2017 via Hootsuite
Contag: Able to translate multi-color fluorescence to H&E simulation. Entire colon, a 'Google Earth pathology' #AACR17
7:43am April 3rd 2017 via Hootsuite
Contag: 2-3 um res, fluorescence imaging. $2.5K in parts instead of $70K, thanks to an ingenious student. #AACR17
Contag: Microscopes in the body - req FDA approval. What about point-of-care? Wide-field microscope, multispectral, filterless #AACR17
7:42am April 3rd 2017 via Hootsuite
Contag: Particles not FDA-approved, although discussions underway. Able to show xenograft pig model data #AACR17
7:41am April 3rd 2017 via Hootsuite
Contag: Aiming for 1mm pixel res. Able to get a 3d representation of the topology; using the Raman noise instead of the signal #AACR17
Contag: Shows 6 separate dyes, a circumferential Raman scope, each of six colors in a circulating fashion (approx size of colon). #AACR17
7:38am April 3rd 2017 via Hootsuite
Contag: Shows spectrum of Raman shift; sharp peaks; can deconvolute. '09 PNAS https://t.co/58nO1d6aHS Want colon imaging in 10' #AACR17
7:35am April 3rd 2017 via Hootsuite
Contag: Washing unbound probe in-vivo also problematic. Have now moved to nanoparticles - surface-enhanced Raman scattering #AACR17
7:34am April 3rd 2017 via Hootsuite
Contag: Small lasers, uPMTs. Shows spectral overlap of different fluor dyes. For single cells is fine; but in-vivo only 2 or 3 #AACR17
7:32am April 3rd 2017 via Hootsuite
Contag: Caled a 'smart jewelry' project - DAC-array to 'smart bracelet' - mobile processing, look at indiv blood vessels #AACR17
7:31am April 3rd 2017 via Hootsuite
Contag: Shows staining of different dyes; 488nm, 560nm, 680nm dyes. Indicates detection of individual CTCs #AACR17
Contag: Targeting a 50um blood vessel; calc of blood flow, math indicates too low a volume. Thus 'wearable flow cytometers' #AACR17
7:30am April 3rd 2017 via Hootsuite
Contag: Imaging thru skin: in mouse ear, shows dye flowing through vessels in real-time. Should be able to detect flow thru vessels #AACR17
7:29am April 3rd 2017 via Hootsuite
Contag: (Thus - count CTCs in-vivo!) Microscope design is a line-scan (instead of prior optical system, a point scan) #AACR17
7:28am April 3rd 2017 via Hootsuite
Contag: Use a microscope for a transect scan - a blood vessel in cross-section; label not only the tumor, but also CTCs w/a dye #AACR17
Contag: And in-line microfluidics. Needed fiducials to determine where tumor is located; uses vasculature as a 3D map. #AACR17
7:27am April 3rd 2017 via Hootsuite
Contag: A resection tool in a microscopic device - 2nd port for 'pulsed electron avalanche knife' (PEAK) #AACR17
7:26am April 3rd 2017 via Hootsuite
Contag: Wanted to use it for medulloblastoma - pediatric brain tumor, use GFP-labeled mAb, VEGFR1 mAb staining of tumor margin #AACR17
7:25am April 3rd 2017 via Hootsuite
Contag: Shows collection of 5 parts - 'putting them in a jewelry box made sense'. One shown w/suture holes (for a mouse model) #AACR17
7:24am April 3rd 2017 via Hootsuite
Contag: Made of nickel, can snap together, in the $100's or $10's range. Shows engineering drawing, dual axis, alignment #AACR17
7:23am April 3rd 2017 via Hootsuite
Contag: 3mos, several $100K to build. Micromachining to lithography - 2D 'old technology' making watch parts. 'LIGA' #AACR17
7:22am April 3rd 2017 via Hootsuite
