May 2018

A2 Figdor: #ampeurope2018 We still don't know at what point T790M level in ctDNA would be the right time to switch.

5:24am May 2nd 2018 via Twitter Web Client

Q: Best time to change Rx? Lemoine: #ampeurope2018 Critical questions - if ctDNA rises, is it relevant? Doing CT imaging, T790M to change Rx

5:24am May 2nd 2018 via Twitter Web Client

Lemoine: #ampeurope2018 50ng input, multplex in 40 PCR/well. Metastatic lung, 100 consecutive plasma at progression.

5:10am May 2nd 2018 via Twitter Web Client

Lemoine: #ampeurope2018 Using tiled probes along ALK and EML4; only products ID'd by size and MSpec and shows result

5:07am May 2nd 2018 via Twitter Web Client

Lemoine: #ampeurope2018 Wanted daily practice: at Dx, at tumor progression, to monitor. Unpredictable breakpts, 150bp amplicons

5:04am May 2nd 2018 via Twitter Web Client

Lemoine: #ampeurope2018 RT-PCR not practical - 30% FN. RNA-Seq: large panels that needs high cost, expertise, large amt of input

5:02am May 2nd 2018 via Twitter Web Client

Lemoine: #ampeurope2018 No info on variants, unable to analyse all vars. IHC is the protein, deps on Ab, and results variable

5:00am May 2nd 2018 via Twitter Web Client

Lemoine: #ampeurope2018 Gold std is FISH: but laborious, not suitable to volume; no automation; consumes expertise

4:59am May 2nd 2018 via Twitter Web Client

Lemoine: #ampeurope2018 Prop of ALK vars could dep on ethnicity: few studies on non Asiatic cohorts. https://t.co/5mm9z4Qx9h

4:58am May 2nd 2018 via Twitter Web Client

Lemoine: #ampeurope2018 Gene fusion biomarkers have analytical difficuties; many new gene fusions like TMPRESS2. Few targeted.

4:55am May 2nd 2018 via Twitter Web Client

Lemoine: #ampeurope2018 Shows workflow - Stg IV NSCLC, if ALK-EML4, if EGFR, or PDL1. Either FFPE tissue or cfDNA

4:52am May 2nd 2018 via Twitter Web Client

Lemoine: #ampeurope2018 Shows effect of 3rd-gen TKI's; shows OS fig from '16 https://t.co/vprtqXnGVy

4:50am May 2nd 2018 via Twitter Web Client

#ampeurope2018 Antoinette Lemoine (APHP Univ Paris INSERM) ALK-EML4 fusions in ctDNA in lung cancer patients in the clinical practice

4:49am May 2nd 2018 via Twitter Web Client

van Schaik: #ampeurope2018 '17 a nat'l 'cell-free DNA day'. Will do another one in Utrecht, 14 June '18. https://t.co/mTjcQeoDUB website

4:45am May 2nd 2018 via Twitter Web Client

van Schaik: AR-V7 and PCa resp to hormone trtmt: PFS exosomes may be more sens than cfDNA #ampeurope2018

4:43am May 2nd 2018 via Twitter Web Client

van Schaik: Acquired MassArray, 67 muts, 5 genes, sensitivie and inbetween ddPCR and cobas WRT price #ampeurope2018

4:38am May 2nd 2018 via Twitter Web Client

Q: NeoAg prediction? Figdor: #ampeurope2018 DNA / RNA seq, ID potential ones, not as high as expected, differ bet. pts.

3:32am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Provocative but TMB is not ready for clinical use; MMRD tumors 40% resp rate

3:30am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Emerging indications: melanoma, lung, renal cell, Hodgkin, H&N, bladder, Merkel cell, MMR-D

3:29am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Q: is it ready for pt care? How reprod? Only one company? Large amt of sample. PD-L1 access - 34%/58% TMB avail

3:26am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 CheckMate 227: Nivo plus Ipi at >10 mut/MB in NSCLC, PFS 43% vs 13% at 12mos. 'A provocative marker'

3:23am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Shows ORR by coding mutations (shown yesterday too) https://t.co/dhqMA0ts8n

3:22am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Mut load correlates with poor clinical outcome to anti-PD-1 in NSCLC. '16 https://t.co/DI0FIQF31L

3:20am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Largest database of its kind, worldwide. https://t.co/soi0zSX7kh

3:17am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Biopsy: WGS 110x, RNA-seq too. Have 2.8k samples of metastatic tumors, with Rx data, avail for research

3:16am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Edwin Cuppen: national scale WGS, a nationwide effort (Hartwig Fndn). Blood (serum, WBC); 38x WGS.

3:15am May 2nd 2018 via Twitter Web Client

Horlings: #ampeurope2018 Ultra-high muts correlate with MMR and POL-E. Looking at hypermutated trinucleotide context - and clustering

3:14am May 2nd 2018 via Twitter Web Client

Horlings: #ampeurope2018 WGS of 560 br ca samples, looking for MMR-D, found 12. looking at MLH1, MSH2, MSH6, PMS2 status, cp to Circos plots

3:09am May 2nd 2018 via Twitter Web Client

Horlings: #ampeurope2018 MMR-D and PD-1 blockade: Le in NEJM '15, Science '17. 'A pan-tumor predictive marker for I-O'

3:05am May 2nd 2018 via Twitter Web Client

Horlings: #ampeurope2018 Slightly higher 9.9 mut/MB in pediatric ca. 17% are above 10, 1% above 100. On the highest: Kidney, Prostate

3:03am May 2nd 2018 via Twitter Web Client

Horlings: #ampeurope2018 81K tumors of 315 cancer-rel genes in '17 https://t.co/4eIkuGgoho Shows threshold of hypermut. 9 mut/MB

3:02am May 2nd 2018 via Twitter Web Client

Horlings: #ampeurope2018 Fig from '14 rev https://t.co/icS9N7xDSm A cancer 'immunogram' https://t.co/HCq73sl2ul

2:57am May 2nd 2018 via Twitter Web Client

Horlings: #ampeurope2018 TIL Rx - exfused, expanded, infused with IL-2; shows photos of a case where dramatic lesions disappearing

2:55am May 2nd 2018 via Twitter Web Client

Horlings: #ampeurope2018 Form '11 https://t.co/DGfZwBzIyL to now: 3y OS of StgIV melanoma chart shown

2:53am May 2nd 2018 via Twitter Web Client

Q: Polymer have a half-life? Figdor: #ampeurope2018 Unknown as of yet, biodistribution studies in mice ongoing.

2:45am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Limited in the Ag's for solid tumors that we have; only two. We can hope that can be expanded.

2:43am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Anticipates all patients will be sequenced; then HLA neoantigens can be predicted. CD4 response impt, will get better

2:40am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Used Vectra multimodal staining, scanning the entire tumor, and det CD3, CD8, CD45, FoxP3, CD45ro, GrzB, tumor

2:30am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 It is the numberof CD3+ T-cells for primary melanoma predicts surv after DC-based I-O https://t.co/TNSEJUETyN

2:30am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 These polymers act like flexible springs, with antibodies and derivatized w/biotin. Shows docked Abs image

2:23am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Uses Toll-Like Receptor. good resp w/both OVA Ag and TLR ligand. Is working with a flexible polymer

2:22am May 2nd 2018 via Twitter Web Client

Figdor: #ampeurope2018 Targets dendritic cells (DCs) with PLGA biogradable polymer, and antibodies etc. '11 https://t.co/prTlDcgNK5

2:18am May 2nd 2018 via Twitter Web Client