Stubbington #AGBT19 Direct VDJ sequences between two cells. TCRa/TCRb, bind to peptide Ag by MHC I or MHC II alpha/… https://t.co/YTBaHdvEhH
12:34pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Fig from '18 Nature Rev Immunol https://t.co/aVb1j3klk8 Partner with Immudex for reagents to ma… https://t.co/7rr8zaTaiv
12:32pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Simultaneous RNA and protein detection in 15,733 cells, from new PDF (not on website yet but in… https://t.co/FxJcqciIBR
12:30pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Feature barcoding: Abs conjugated to barcodes. Partnered with BioLegend for Ab's.
12:29pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Coming soon - compatible products from Millipore Sigma. Second: quantifying cell surface proteins.
12:27pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Points to recent '19 Cell https://t.co/7v0yHgjQsp from Shendure's group.
12:26pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Barcoding 'things' - CRISPR perturb-seq. Introduce pool of gRNAs into a pool of cells, examine… https://t.co/l7l6NFlfAs
12:25pm February 28th 2019 via Twitter Web Client
Mike Stubbington (10X Genomics) #AGBT19 Workshop Increased sens with sc gene expression v3. A year ago: announced c… https://t.co/9dQsh7WEyJ
12:23pm February 28th 2019 via Twitter Web Client
10X Workshop #AGBT19 what's next? sc CNV / epigenomics / spatial gene expression / surface protein expression
12:22pm February 28th 2019 via Twitter Web Client
10X workshop at #AGBT19 Last example - mechanisms of I-O resistance from https://t.co/JUOH310xK4 Mechanism persists… https://t.co/DZtww23dYx
12:21pm February 28th 2019 via Twitter Web Client
Lau #AGBT19 Click chemistry conjugations of analytes onto solid phase; target enrichment and elution. Uses modified… https://t.co/hti9TqWTlT
11:53am February 28th 2019 via Twitter Web Client
Billy Lau (Stanford) #AGBT19 A DNA 'time machine' for repeated interrogation of cell-free DNA and FFPE. Approach: s… https://t.co/9qfRylDLha
11:52am February 28th 2019 via Twitter Web Client
Ettwiller #AGBT19 CAGE detects only TSS from Pol II transcripts. Other Pols (I, II, III and mitochondrial pol) prod… https://t.co/onDOjI0JYi
11:51am February 28th 2019 via Twitter Web Client
Lee Ettwilller (NEB) #AGBT19 Poster 503 ReCappable Seq for TSS interrogation. TSS better define transcripts, and ac… https://t.co/f7385FOFbs
11:49am February 28th 2019 via Twitter Web Client
Shapiro #AGBT19 Also points to this '17 Nature Methods using a method called histoCAT https://t.co/LlCew8cKkW
11:43am February 28th 2019 via Twitter Web Client
Shapiro #AGBT19 Induced in epith compartment. '18 Cell CODEX https://t.co/JaQYEljIql and points to BioRxiv of GeoMx https://t.co/GW80Eo2U51
11:41am February 28th 2019 via Twitter Web Client
Dennis Shapiro (Broad) #AGBT19 Poster 119 Reconstruction of the colorectal cancer landscape. Induced APC deletion,… https://t.co/15A0tui9kE
11:40am February 28th 2019 via Twitter Web Client
Chen #AGBT19 Poster 103, extracting longitudinal data from a single lung patient. Drill-down into timeline, automat… https://t.co/xeiOLkKIF0
11:38am February 28th 2019 via Twitter Web Client
Chen #AGBt19 phot of the Centrellis Sema4 platform. Clinical test, Pt mgmt, Rx. https://t.co/3hCQ0I0Lil
11:37am February 28th 2019 via Hootsuite Inc.
Flash talk 1 #AGBT19 Rong Chen (Sema4) Advancing precision medicine through data science. Centrellis Health Intelli… https://t.co/FwIjfGVbS8
11:36am February 28th 2019 via Twitter Web Client
Q: Library prep? PE? Clark #AGBT19 Standard approaches; SE to begin with, PE sometime in the future.
11:32am February 28th 2019 via Twitter Web Client
Clark #AGBT19 Small, inexpensive, scalable, real-data, range of sample types. Can 'sense nucleic acids, proteins and cells'. H2 '19 launch.
11:31am February 28th 2019 via Twitter Web Client
Clark #AGBT19 The cost and batches diagram. https://t.co/CEXL3yHS7C
11:30am February 28th 2019 via Hootsuite Inc.
Clark #AGBT19 Also data from Hudson Alpha, very similar results.
11:29am February 28th 2019 via Twitter Web Client
Tilgner #AGBT19 Plot of Q-scores by position in read form Stanford - well-above 30, until the end. JAX data similar… https://t.co/6EGbuU30ej
11:28am February 28th 2019 via Twitter Web Client
Clark #AGBT19 Same sample on AmpliSeq CHP v2, R2 = 0.992 for expected vs measured AF. Next - cell-free DNA from a transplant pt.
11:24am February 28th 2019 via Twitter Web Client
Clark #AGBT19 Using HDx's HD701 cancer genes at low AF, very good agreement at 1.0%, 2.0%, 3.0%.
11:23am February 28th 2019 via Twitter Web Client
Clark #AGBT19 SNP concordance for NA12878 exome. Very good overlap at 66x GenapSys cp to ILMN 280X coverage. Subst… https://t.co/gFxJYSUJSd
11:22am February 28th 2019 via Twitter Web Client
Clark #AGBT19 Comparison by GC content on GenapSys https://t.co/FQv0T10Gve
11:21am February 28th 2019 via Hootsuite Inc.
Clark #AGBT19 Density - 144M sensor chip. Now onto data: E Coli: 12-15M active sensors, ave readlength 100-150bp. Raw read accuracy >99%.
11:21am February 28th 2019 via Twitter Web Client
Costco #AGBT19 Photo of Readlength distribution and cumulative accuracy. https://t.co/lwvM5XTcLO
11:20am February 28th 2019 via Hootsuite Inc.
Clark #AGBT19 (Even refers to flows, same nomenclature as Ion.) Steady-state signal, can bee summed to give differe… https://t.co/B0xBhrj3tR
11:18am February 28th 2019 via Twitter Web Client
Clark #AGBT19 Start with clonally amplified; incorporation is 'signal or not'. 'Electronic sequencing by synthesis'… https://t.co/3qrCwNylS9
11:16am February 28th 2019 via Twitter Web Client
Clark #AGBT19 Smallest is 1M, middle is 16M, largest is 144M. Box about $10K, per-run is "hundreds of dollars, not thousands".
11:15am February 28th 2019 via Twitter Web Client
Clark #AGBT19 About the size of an iPad. "Proven measurement accuracy of >99%". 3 chips, 3 different sensors (to 14… https://t.co/VFnkEwW
11:14am February 28th 2019 via Twitter Web Client
Clark #AGBT19 (With this slide I feel like I have heard the message of democratization before...) https://t.co/tTCdDk21iA
11:14am February 28th 2019 via Hootsuite Inc.
Tyson Clark (GenapSys) #AGBT19 Electrical detection of single base incorporations enables high accuracy sequencing… https://t.co/SJRqrUNAwq
11:11am February 28th 2019 via Twitter Web Client
Tilgner #AGBT19 Hybrid methods give up single-molecule methods. After short-read correction 1.25% for ONT; PacBio 0… https://t.co/PF0uQsrMUX
11:06am February 28th 2019 via Twitter Web Client
Tilgner #AGBT19 Shows PromethION reads - 31Gb, 31M, 10-15% error rate. Fraction of reads where find barcode: ~40% f… https://t.co/lU3cyT34N4
11:05am February 28th 2019 via Twitter Web Client
Tilgner #AGBT19 PromethION, PacBio's 8M ZMW: shows data 107Gb to 395Gb per SMRT-cell. No of CCS reads - 1.4M to 4.6… https://t.co/7WHqY8Akug
11:04am February 28th 2019 via Twitter Web Client
Tilgner #AGBT19 Introduced errors on-purpose on barcodes, determined 99.99% specificity. https://t.co/dCH2hiAgwb an… https://t.co/LeVhWhz0ee
11:03am February 28th 2019 via Twitter Web Client
Tilgner #AGBT19 ScISOr-Seq - single-cell isoform RNA-Seq '18 NBT https://t.co/8PdGsz7aUG Use 10x up-front, Illumina… https://t.co/r8bsOw7Yjv
10:58am February 28th 2019 via Twitter Web Client
Tilgner #AGBT19 Presents data from this '15 NBT https://t.co/gcKjAhEvw4 and then '18 Genome Res https://t.co/MqzOc3hAj4
10:56am February 28th 2019 via Twitter Web Client
Hagen Tilgner (Weill Cornell) #AGBT19 Isoform sequencing in tissues, cell types and thousands of cells
10:54am February 28th 2019 via Twitter Web Client
New post for #AGBT19 - You can outsource just about anything. Profiling Directed Genomics, @argonaut_ms,… https://t.co/mzDHCs4jar
New post: You can outsource just about anything https://t.co/df93DFMqTx
10:36am February 28th 2019 via WP to TWTR on Yuzuki.org
Regev: #AGBT19 Preprint on DNA Microscopy on bioRxiv https://t.co/7bR6OsULm1
9:33am February 28th 2019 via Twitter Web Client
Regent #AGBT19 Summary of toolbox - and DNA microscopy on BioRxiv https://t.co/m9PbF0aGjF
9:32am February 28th 2019 via Hootsuite Inc.
Regev #AGBT19 Collab with Fei Chen at MIT, shows data that it works, out of time...
9:31am February 28th 2019 via Twitter Web Client
Regev #AGBT19 Method of compression published '17 Cell https://t.co/ovt64eKu4F "It sounds like black magic but it actually works."
9:30am February 28th 2019 via Twitter Web Client
