Celsee #AACR19 Ranade 250K microwells, 25K cells loaded. Capture eff: irrespective of scale (2.5K cells to 22.5K cells)
3:11pm April 2nd 2019 via Twitter Web Client
Celsee #AACR19 Ranade Bead loading is simple via gravity; cap put on, excess beads washed. Cells lysed, mRNA hyb to… https://t.co/XWW0GfcAiv
3:10pm April 2nd 2019 via Twitter Web Client
Celsee #AACR19 Ranade Dissociate tissue, optional Ab tagging of surface proteins. pipette onto slide, optional QC via microscopy.
3:09pm April 2nd 2019 via Twitter Web Client
Celsee #AACR19 Ranade Celsingle is open microwell slide; hexagonal features; recover >70% of input cells.
3:08pm April 2nd 2019 via Twitter Web Client
Celsee #AACR19 Ranade Also open platform - two different types of slides: Celsingle slide, Preparation slide. (Firs… https://t.co/1Ogl81GRwS
3:07pm April 2nd 2019 via Twitter Web Client
Celsee #AACR19 Ranade Building tools on sc technologies: addresses deficits - gravity is gentle, visual cell-loadin… https://t.co/rCcHobAJi9
Celsee #AACR19 Ranade: Is developing a system called Genesis; starting with the 'why': sc approaches gives understanding of heterogeneity
3:06pm April 2nd 2019 via Twitter Web Client
#AACR19 Celsee Workshop: Swati Ranade (Dir R&D), Bruce Patterson (CEO Incell Diagnostics) Andrew Adey (OSHU)
3:04pm April 2nd 2019 via Twitter Web Client
Thompson #AACR19 Concl - a powerful new tools to map the immune landscape of breast tumors; stromal compartments ma… https://t.co/g7jmWWtCtb
10:40am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 Shows heatmap of FN1, CD86, IDO1 - myeloid and ECM markers in CD68-enriched assoc'd with recurr-free survival
10:37am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 CD68enriched Myeloid/macrophage enriched stroma associated with positive outcome. One gene robustl… https://t.co/A8bjn933sk
10:36am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 Clear differences between tumor and stroma; shows PCA plot, looks at GEX of PD-L2 in CD68 enriched… https://t.co/E6fjblP4JG
10:34am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 300 um ROI chosen by hand; mask for PanCK+; draw for SYTO12+/PanCK-. Shows S:N numbers (protein) a… https://t.co/oXj5r4DwpP
10:30am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 12 ROI's, 42 target proteins, 5 segments/sample. PanCK, CD45, CD68, SYTO13. (tumor, leukocytes, ma… https://t.co/Z7N3xk3lIo
10:28am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 BC360/IO360 has generated a lot of observations; giving the imptc of looking at TME. Shows chart o… https://t.co/4SOPys6aXP
10:26am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 FinXX trial - 120 TNBC, two arms T + CEF (5-fluoruacil) / Tx + TEX (capecitabine). Profile immune… https://t.co/bfB6BsLCRc
10:25am April 2nd 2019 via Twitter Web Client
Aubrey Thompson (Mayo Clinic FL) #AACR19 Had the instrument for about 6w; not an immunol but working on gene expression.
10:23am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 AbCam a partner, will be making antibodies compatible with GeoMx; ACD another with FFPE-compat sing… https://t.co/INdG9HD7QQ
10:20am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 Digital, multi-analytie, single-molecule counting, 10^6 dynamic range, single-cell limit of detecti… https://t.co/KdzAgBUaZz
10:18am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 illustrating active va inactive invasive margins. https://t.co/HVQQXojtvM
10:17am April 2nd 2019 via Twitter for iPhone
Beechem #AACR19 Two different invasive boundaries compared: at the GEX level, completely different.
10:16am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 Coming soon: unlimited multiplexing; showing 1500 genes Q4, in lab above 10,000 genes. Shows 1412 g… https://t.co/Se40PIt95A
10:15am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 Shows different types of profiling - along a boundary, with a gradient. Two of four references:… https://t.co/4dAxVmGROu
10:14am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 PanCK lights up tumor-spec cells; mirrors will collect only those cells. Image can be reversed - only TME; 30s apiece.
10:09am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 All antibodies applied; then four colors illuminate ROI. Tags collected w/ x-y coordinates. Deliver… https://t.co/MFvEvgJYty
10:07am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 The microscope not only the reader, but also capable to cleave the tags and collect with x-y coordinate info.
10:05am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 Instead of colors, use short oligo tags to encode the ID of Ab or mRNA sequence. And the barcode ta… https://t.co/gZigXgmYBl
10:04am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 Continuous discovery cycle: bulk gene exp to spatial profiling. 5y ago the concept: measure 10K things in a microscope.
Beechem #AACR19 The idea: same basic idea, using digital optical profiling, and using the technology and moved into spatial domain.
10:02am April 2nd 2019 via Twitter Web Client
Joe Beechem (Nanostring) @nanostringtech Multi-analyte profiling using the GeoMx digital spatial profiling. #AACR19
Resolution Liquid Biopsy Assay Outperforms Guardant360 in Retrospective Non-Small Cell Lung Carcinoma Comparison Pi… https://t.co/KKxrHx2DSF
8:32am April 2nd 2019 via Buffer
Heath #AACR19 B-cell antigens have discontinuous epitopes. The challenge - most important yet most complicated to get to.
7:46am April 2nd 2019 via Twitter Web Client
Heath #AACR19 CD Tcell antigens are becomign increasingly well understood - like a hotdog in a bun. CD4 T cell anti… https://t.co/FuVbrkwW9w
7:45am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Shows pMHC library construction and protein production - cost-effective, very specific.
7:43am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Engineered a single-chain tetramer, called signalling and antigen presenting bifunctional receptors (… https://t.co/RQ8ZfZeYgn
7:42am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Shows flow data that suggests low concentration of TCR. Back to tetramer approach (Schumaker group) -… https://t.co/BEwo22UWQ3
7:38am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Could be seeing low-binding affinity or low T-cell populations. KOKIKO - knock-out, (TCR) knock-in an… https://t.co/OjyjKZsygn
7:36am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Shows data from a poster at this conference; seeing T-cell populations via this sc method not seen by std tetramer-based flow.
7:34am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Kinetic study of neoantigen-spec PBMCs. Melanoma pt with anti-PD1 Rx; shows TILS at d187, shows CD8+… https://t.co/OmRz27um3n
7:30am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Fluorescent microscopy 'not a lot more painful than flow but a lot more sensitive'.
7:28am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Nanoparticle gives scaffold for 20k pMHC tetramers per particle (!) Can isolate via hemocytometry chi… https://t.co/hVwiaPR8KB
Heath #AACR19 Can use minigene approach, what antigens activated; or flow-based sorting; fastest is conditional ant… https://t.co/g5YbQqnC3O
7:27am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Part 2: Sensitive detection of antigen-spec T-cell populations and pairing of antigen-MHC with TCRa/b… https://t.co/u2TbJ0jCw1
7:26am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Knowledge of trajectories w/sc fluctuations, provides druggable targets for each trajectory.
7:24am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Concl of part 1: isogenic cells can take multiple indep trajectories to drug tolerance. Trying to det… https://t.co/TYOzeFnqKs
Heath #AACR19 If the two trajectories are indep: drugging of one type is indep of the other, and shows data PKM2i vs NFKBi.
7:23am April 2nd 2019 via Twitter Web Client
Heath #AACR19 (NB: I see from the slides that Dr. Heath is at the ISB in Seattle since 2018.)
7:21am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Shows time-lapse video of MITF-GFP high and MITF-GFP low, and a separation between states: 'no stocha… https://t.co/VpBcx37JsX
7:20am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Shows constraints in their cell-state mapping of the single-cell protein and metabolite data https://t.co/iUN82BapFH
7:18am April 2nd 2019 via Twitter for iPhone
Heath #AACR19 Shows new statistical method similar to PCA, find two unbalanced processes.
7:17am April 2nd 2019 via Twitter Web Client
