Mouritzen: Able to get >80% mappability. 25% miRNAs; reviews pro's and con's of qPCR cp to NGS; hypothesis vs. hypothesis-free #Tricon
4:25pm February 20th 2017 via Hootsuite
Mouritzen: Also dev RNA spike-ins for monitoring cDNA synthesis, isolation efficiencies, and lib prep efficiency (52 spp of spikes) #Tricon
4:20pm February 20th 2017 via Hootsuite
Mouritzen: Thus specialized pur kits, LNA-based qPCR, and optimized lib preps #Tricon
4:19pm February 20th 2017 via Hootsuite
Mouritzen: Limited amts of RNA; PRC inhibitors; cellular contam. and hemolysis are challenges for microRNAs #Tricon
Mouritzen: Stability due to assoc'n w/exosomes fig from '07 ref https://t.co/IxN0UbJOvv #Tricon
4:18pm February 20th 2017 via Hootsuite
Mouritzen: microRNAs are 'excellent diag biomarkers' due to exosomes, stability, biological potential, limited number #Tricon
4:16pm February 20th 2017 via Hootsuite
Molecular #Tricon 2017 starting wtih Exiqon Peter Mouritzen RNA in Liquid Biopsies - promising new biomarkers of diseases
4:15pm February 20th 2017 via Hootsuite
New Zealand is the tip of a ‘hidden continent.’ - The Washington Post https://t.co/iQl63tRS8u
2:10pm February 20th 2017 via Hootsuite
How mammoth cloning became fake news – Medium https://t.co/fb4pXrkUcj
1:05pm February 20th 2017 via Hootsuite
MT @Xtalks: Enabling Clinical #Genomics with Highly Multiplexed and Patient-like Reference Materials w/ @SeraCare https://t.co/X6hSpBQ4WX
11:30am February 20th 2017 via Hootsuite
Forget genius. Science is the product of less-than-brilliant minds - LA Times https://t.co/PwV8lmqoRq
11:10am February 20th 2017 via Hootsuite
Cautious Optimism (Dec 2015): Lessons from the Cognitive Front Lines: Early Adopters of IBM’s Watson WSJ https://t.co/EL1PMtPYDm
10:46am February 20th 2017 via Hootsuite
RT @DrKhouryCDC: Can solid tumor genome sequencing ID mutations that improve clinical outcomes? Via @PLOSMedicine https://t.co/qOxEf2XX0K
10:39am February 20th 2017 via Hootsuite
Background on Watson's AI: Why go to the Moon when what your company really needs is in the Rockies? Kyield https://t.co/W5kkevdCuw
10:02am February 20th 2017 via Hootsuite
MD Anderson and IBM Watson's Oncology Expert Advisor: a $62M investment in healthcare AI goes sour | Forbes https://t.co/bX8chPC65j
9:29am February 20th 2017 via Hootsuite
A survey of 53 attendees at #AGBT17: what caught your attention? @DeciBio https://t.co/txlMzFMVWo
7:19pm February 18th 2017 via Hootsuite
For all you at #AGBT17 RT @DaleYuzuki: New post: Bionano Genomics goes human-genome scale https://t.co/8fUHkUl4li
7:11pm February 18th 2017 via Hootsuite
Prepare to Obsess Over the Future of Home Wifi | Medium https://t.co/qflXKPUYW8
3:22pm February 18th 2017 via Hootsuite
Beware, the content goes deeeeep... RT @OmicsOmicsBlog: #AGBT17 Tweet Archive is Up! https://t.co/frBl0z0oLG
8:46am February 18th 2017 via Hootsuite
RT @NatureNews: Four reasons the CRISPR patent verdict isn’t the end of the story. https://t.co/gU0tdPZD7Q https://t.co/cefWo1ZLpe
5:10pm February 17th 2017 via Hootsuite
MT @moorejh: Mining the Human Gut Microbiota for Immunomodulatory Organisms - Cell https://t.co/bPCNFFMVh1 https://t.co/HxDGBreNnN
4:10pm February 17th 2017 via Hootsuite
RT @NewsfromScience: The H7N9 avian flu is spreading in China: https://t.co/RqFX1GPUCH
2:57pm February 17th 2017 via Hootsuite
Woolly mammoth on verge of resurrection, scientists reveal | The Guardian https://t.co/L4jQ8PYiuc
11:15am February 17th 2017 via Hootsuite
DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification | Science https://t.co/7fQlDUwRnU
10:02am February 17th 2017 via Hootsuite
RT @coregenomics: #agbt17 Tweeters... @DaleYuzuki @jgreid @tysonaclark @mike_schatz @mason_lab @Supergecko and me! https://t.co/S5MMUlOZqB
6:41pm February 16th 2017 via Hootsuite
The Next Pseudoscience Health Craze Is All About Genetics | Gizmodo https://t.co/J0INorTkRu
5:33pm February 16th 2017 via Hootsuite
Thanks for all the great discussion #AGBT17 - hope to see you next year!
4:15pm February 16th 2017 via Hootsuite
Green: #AGBT17 The first Precision Health meeting was an experiment; all invited to the 2nd year Sept 14-16 in Scottsdale AZ
4:14pm February 16th 2017 via Hootsuite
@mason_lab @M_L_Carpenter @TorontoGenomics @coregenomics @dgmacarthur @obahcall Alas headed back to DC right away. :(
4:13pm February 16th 2017 via Hootsuite in reply to mason_lab
Q: Live cells? Daugherthy: Something they are thinking about; toxicity and getting probes into cells will be difficult #AGBT17
4:09pm February 16th 2017 via Hootsuite
Q: Viral sequences? Daugherthy: Doing that work now supported by Gates #AGBT17
Here's an interesting Vimeo of Readcoor's technology (with George Church) https://t.co/vUikIn98CZ #AGBT17
4:05pm February 16th 2017 via Hootsuite
RT @coregenomics: ED: maybe run a cool workshop like @nanostringtech can I be the guineapig again? @readcoor #agbt17 https://t.co/4B9CCHz6lI
4:04pm February 16th 2017 via Hootsuite
Daugherthy: Goal is to look at DNA, RNA, protein. Announces: Human Cell Atlas https://t.co/INtbrkT7Cl and Precipitatebio #AGBT17
4:02pm February 16th 2017 via Hootsuite
Daugherthy: Est they can now detect about 10K unique molecules/cell. Are developing automation. Show prelim data of DNA, protein #AGBT17
3:59pm February 16th 2017 via Hootsuite
Daugherthy: Can find biologically meaningful base change data; gives example of intentional wounding and a healing signature #AGBT17
3:54pm February 16th 2017 via Hootsuite
Daugherthy: Then RCA, called a 'rolony' - representing sequence plus position #AGBT17. RNA-FISSEQ is RNA-Seq data.
3:51pm February 16th 2017 via Hootsuite
Daugherthy: Shows impressive 3D video (with colors!) nice visuals. https://t.co/ME0388QEca Fix RNA, RT primer, extend, circularize #AGBT17
3:49pm February 16th 2017 via Hootsuite
Daugherthy: FISSEQ - spatial information rather than just a list. Original Church Science paper '14 https://t.co/YGFIAyrpvi #AGBT17
3:46pm February 16th 2017 via Hootsuite
Evan Daugherthy (Harvard Univ MA) A fully automated massively-multiplex panomic in-situ sequencing #AGBT17
3:45pm February 16th 2017 via Hootsuite
RT @shgoodwin1: Last point - in bold - in Rosenfeld talk. Be carefull where you buy sperm. Only at #agbt17
3:43pm February 16th 2017 via Hootsuite
RT @morgantaschuk: The amazing NASA biomolecule sequencer patch that @mason_lab showed at #agbt17 https://t.co/IZdHfpr8vp
Rosenfeld: Used 10X method; of 8 cases, 8 controls, only got 5 cases and 3 normals. Evidence of 65K sperm from 1 sample #AGBT17
3:35pm February 16th 2017 via Hootsuite
Rosenfeld: Shares feedback of sperm banks regarding cases of autism. Shows donor sibling registry, anonymizing. 50 to 100 kid/donor #AGBT17
3:33pm February 16th 2017 via Hootsuite
Rosenfeld: Why: haploid; easy to obtain; only biological contribution from the father; can determine raw mutation rate. #AGBT17
3:30pm February 16th 2017 via Hootsuite
Jeff Rosenfeld (Rutgers Univ NJ) Single-cell RNA sequencing in sperm from fathers of autistic children #AGBT17
3:29pm February 16th 2017 via Hootsuite
Nolan: Setup a company called Apprise Bio to move the technology forward. https://t.co/7s1vCECmWW #AGBT17
3:26pm February 16th 2017 via Hootsuite
Nolan: Barcoding 10's of million cells, can be barcoded in a few hours. Ab reads are better than FACS, equiv to CyTOF; DNA and RNA #AGBT17
3:25pm February 16th 2017 via Hootsuite
Nolan: Ultra-rare cell seq: (1 in a million). You need is one known 'hook'. PCR hook with known, cell barcode can go back an pull #AGBT17
3:24pm February 16th 2017 via Hootsuite
Nolan: New RNA method: proximity ligation approach, multiple RNA targets. (Called 'Snail'.) #AGBT17
3:22pm February 16th 2017 via Hootsuite
