Beechem: For clinical sequencing: FFPE melting, remove particulates via filter, then apply to sequencer. #AGBT17
12:20pm February 15th 2017 via Hootsuite
Beechem: No enzymology: no library amplification, cluster generation. Can use FFPE, lysates, blood, tissue sample types #AGBT17
12:19pm February 15th 2017 via Hootsuite
Beechem: Wanted to go from FFPE to targeted sequencing to <1h w/<15min hands-on time. Enables long- and short-reads simultaneously #AG
12:18pm February 15th 2017 via Hootsuite
Beechem: Will talk about the why, how and what next. His own history - before NanoString in '12 was at Life Tech '00-'12. #AGBT17
12:17pm February 15th 2017 via Hootsuite
@coregenomics and Michael Rhodes of @nanostringtech showing live video starting an FFPE prep at #agbt17 https://t.co/C7B4BbEpXj
12:16pm February 15th 2017 via Hootsuite
Beechem: He too James Hadfield @coregenomics and will show a live feed (!) of him sequencing. Shows a slide of the workflow #AGBT17
12:14pm February 15th 2017 via Hootsuite
Beechem: "We're going to do a crazy thing. I've done a lot of PCR etc., and we've erased all that stuff" #AGBT17
12:12pm February 15th 2017 via Hootsuite
NanoString Workshop:Joe Beechem (R&D) Hyb & Seq technology: a no amp, no library, single molecule sequencer designed for the clinic
Levin: PTCHD1 muts found in humans w/autism, attn deficit, similar to mice KO. '16 ref https://t.co/6mSzSYtOk2 #AGBT17
10:09am February 15th 2017 via Hootsuite
Levin: Shows role of TRN between cortex and thalamus - attention, sensory processing, sleep. All disrupted in psych disorders #AGBT17
10:08am February 15th 2017 via Hootsuite
Joshua Levin (Broad MA) Single cell profiling the thalamic and reticular nucleus (TRN) implicated in schizophrenia and autism #AGBT17
10:07am February 15th 2017 via Hootsuite
(Many Q's relate to how work can be expanded, no further data.) #AGBT17
10:06am February 15th 2017 via Hootsuite
RT @ScienceJill: Emma Teeling of @BatLabUCD - Pretty sure this is the best Twitter avatar ever! #agbt17 https://t.co/PUbdtqY98c
RT @timp0: "Bonferroni correction, the murderer of all decent data" - ET about gene evolution in bats for telomere maintenance #agbt17
10:01am February 15th 2017 via Hootsuite
Teeling: Next looking at 50+ samples for whole transcriptome from 0-5 years. Acknowledges groups in Germany, France, Portugal #AGBT17
10:00am February 15th 2017 via Hootsuite
Teeling: Looked at other telomere-assoc'd genes. didn't find any genes evolving under positive selection. Divergence: ATM, SETX #AGBT17
9:55am February 15th 2017 via Hootsuite
Teeling: Telomerase expression in bats? Why don't bats get cancer? Looked for TERT expression via RNA-Seq, undetected #AGBT17
9:53am February 15th 2017 via Hootsuite
Teeling: Humans, badgers, seals, chimps: telomeres all shorten w/age. Found populations - 60y of data on bats, studied smpls #AGBT17
9:52am February 15th 2017 via Hootsuite
Teeling: Uses qPCR for long and short telomeres, related to cT. Age and length ' in 45 individuals 'do not shorten w/age' #AGBT17
9:50am February 15th 2017 via Hootsuite
Teeling: Looking at telomeres, and blood transcriptome. Telomere analogy - a shoelace aglet as it wears down... #AGBT17
9:49am February 15th 2017 via Hootsuite
Teeling: Fed mealworms, wing membrane regenerates quickly. Have 3K wing punctures. Able to do population pedigree #AGBT17
9:48am February 15th 2017 via Hootsuite
Teeling: Know locations, catch same individuals, a big 'sock' to have the fly down (i the castle), moved to boxes. Wing membrane #AGBT17
9:47am February 15th 2017 via Hootsuite
Teeling: Locale in France (shows famous photo of Brittany), shows cool video of 3AM processing sleeping bats. 10y of work #AGBT17
9:46am February 15th 2017 via Hootsuite
Teeling: Needed unbiased, non-invasive sampling. Females return to same place every year to same place to breed. 200uL blood #AGBT17
Teeling: 2GB genome, 22k genes, 1/3 repetitive content. '13 Science https://t.co/1rX1Li8FJ3 Age a bat: catch as babies, tagged #AGBT17
9:45am February 15th 2017 via Hootsuite
RT @agbt: Incomparable @EmmaTeeling1 gives riveting talk on the secrets of everlasting youth. #bats #agbt17 https://t.co/61x7XEekQd
9:43am February 15th 2017 via Hootsuite
RT @coregenomics: NJ: "1 in 5 mammals are bats" this is going to be THE fact I remember from #agbt17
Teeling: Suggest that bats are unusually long-lived for their size; also contain Ebola, Rabies, SARS, MERS. #AGBT17
9:42am February 15th 2017 via Hootsuite
Teeling: Shows a 42y old bat, only 5g large, tagged and re-caught decades later. '10 ref https://t.co/LYXXJ225wI bats and mass #AGBT17
Teeling: Bowhead whale '15 ref https://t.co/AYsvjMX2UW , live long; sharks >400y old. Bats: Found everywhere #AGBT17
9:40am February 15th 2017 via Hootsuite
Teeling: While mice live longer, are 30% smaller and have many other side-effects. Nature may have solved some problems already #AGBT17
9:38am February 15th 2017 via Hootsuite
Teeling: Need to study 'extended health-spans'. Rapamycin discovered on Easter Is in the '60's. Fed to mice, acts on mTOR. #AGBT17
9:37am February 15th 2017 via Hootsuite
Teeling: Shows this TIme cover - baby could live to 142 https://t.co/5uDNoPgVL8 The catch: probability of disease stays constant #AGBT17
9:36am February 15th 2017 via Hootsuite
Emma Teeling (Univ Coll Dublin IR) Comparative genomics of bats: the secret of extended longevity? #AGBT17
9:35am February 15th 2017 via Hootsuite
Q: Looking at transcriptome? Jabado: The data gets dirty, thus interest in single-cell. #AGBT17
9:32am February 15th 2017 via Hootsuite
Jabado: Oncohistones - may be reversible, drugs are under development. Makes the case for open-access data #AGBT17
9:30am February 15th 2017 via Hootsuite
Jabado: Recent work '17 Nature Gen https://t.co/6QNOnEIsAm and this work missed by TCGA as this kind of epigenetics wasn't done #AGBT17
9:28am February 15th 2017 via Hootsuite
Jabado: She makes the point that she was called a 'research parasite' by NEJM, which badge she wears proudly... #AGBT17
9:27am February 15th 2017 via Hootsuite
Jabado: Implicated in other cancers. H3.3K36M/I further work in undiff sarcoma '16 Science https://t.co/atEGmixfLq #AGBT17
9:23am February 15th 2017 via Hootsuite
Jabado: No HTERT promoter mutations in children. '12 Science ref https://t.co/2CJFFkgXDO substitution/knockout experiment #AGBT17
9:20am February 15th 2017 via Hootsuite
Jabado: Mutations in children in gliomas 'is a story of development gone wrong'. K27M Histone 3.1: 'the best partner in crime' #AGBT17
9:16am February 15th 2017 via Hootsuite
Jabado: IDH1 mutation (R132 mut) creates 2-hydroxybutryate, affects lysine demethylation, affects Lys36. #AGBT17
9:13am February 15th 2017 via Hootsuite
Jabado: K27M 'is a death sentence' for pediatric glioblastoma. Shows OS chart from prior paper. #AGBT17
9:12am February 15th 2017 via Hootsuite
Jabado: Complex figure has something like 20 different marks. Used 450K methyl array. Fig '12 ref https://t.co/9hs7g9XdFa #AGBT17
9:11am February 15th 2017 via Hootsuite
Jabado: Complex interplay of histone modeling.Crazy-complex active transcription marks w/Histone 3 - H3K27 repress H3K36 activating #AGBT17
9:09am February 15th 2017 via Hootsuite
Jabado: Epigenome - the new code - where histones play a role. Human has at least Histon 3 var's. Methylation, acetylation, others #AGBT17
9:08am February 15th 2017 via Hootsuite
Jabado: Goes back to nuclear org, how histones wrap DNA, comprising the nucleosome; there are chromatin-binding and remodelers #AGBT17
Jabado: Starting with pediatric glioblastomas, points to Histone 3.3 and TP53 mutations in '12 Nature ref https://t.co/vJLz2alz57 #AGBT17
9:07am February 15th 2017 via Hootsuite
Nada Jabado (McGill Univ CAN) Oncohistones: professional hijackers of the epigenome? #AGBT17
9:05am February 15th 2017 via Hootsuite
Elaine Madrid greeting everyone this AM at #AGBT17 https://t.co/BgWs8JpZtN
9:04am February 15th 2017 via Hootsuite
