Tweets from Hootsuite

MissionBio workshop con't: Catherine Smith (UCSF CA) Singel cell seq of polyclonal resistance to FLT3 inhibitors #AACR18

4:15pm April 17th 2018 via Hootsuite

Eastburn: #AACR18 Then PCR: amplicons produced. Access to gDNA restricted by chromatin. 19-gene AML panel 50 amplicons. 47 gene Myeloid

4:12pm April 17th 2018 via Hootsuite

Eastburn: #AACR18 Proteases digests histones, needs to be inactivated. Cell ID barcodes, PCR reagents, gene-spec primers added.

4:08pm April 17th 2018 via Hootsuite

Eastburn: #AACR18 Microfluidic cartridges: two-step workflow for cell encapsulation, barcoding. Cell lyste inside droplets, merge w/reagents

4:07pm April 17th 2018 via Hootsuite

Eastburn: #AACR18 Tapestri targets 300 loci, across 10k cells/sample. Enables sensitivity for rare clonal populations. Custom content

4:05pm April 17th 2018 via Hootsuite

Dennis Eastburn (MissionBio CA) #AACR18 Intro to technology: single-cell analysis can enable pt stratification, Rx selection, monitoring

4:04pm April 17th 2018 via Hootsuite

Dennis Li, Christina Alix-Panabieres, and Dolores Di Vizio at a Liquid Biopsy roundtable at #AACR18 https://t.co/nNnN8Dvm54

3:30pm April 17th 2018 via Hootsuite

Di Vizio: Mentions need for standardization, controls for EV studies. #AACR18

3:22pm April 17th 2018 via Hootsuite

Di Vizio: Mentions Apogoee/Nanostics for microflow cytometry to look at EVs from PSA levels in Pr Ca pts AUC of 0.84 #AACR18

3:19pm April 17th 2018 via Hootsuite

Di Vizio:Shows unpublished work of WGS of PrCa-derived EVs, and somatic CNVs, and then ddPCR for specific driver gene copy #'s #AACR18

3:14pm April 17th 2018 via Hootsuite

Di Vizio: Large EVs float to 1.10-1.15g/mL fx iodixanol centrifugation gradients. 'Std and ancient methods' https://t.co/t3T5nlKqt2 #AACR18

3:06pm April 17th 2018 via Hootsuite

Di Vizio: #AACR18 '12 Large oncosomes ref https://t.co/D9vQraltFi in mProstate Ca. >1um size, correlated with advanced disease

3:04pm April 17th 2018 via Hootsuite

Di Vizio: A huge need for standardization. '18 https://t.co/9vQs81RUl5 smallest 'exomere' 35nm! impt in cancer Large oncosomes #AACR18

3:03pm April 17th 2018 via Hootsuite

Di Vizio: '16 rev https://t.co/M6X1gHz6Oj EV's impt mediators of intercellular communication. Many types of EVs, affect target cells #AACR18

3:00pm April 17th 2018 via Hootsuite

Dolores Di Vizio (Cedars Sinai/UCLA CA) Cancer-derived extracellular vesicles and liquid biopsy: What have we learned? #AACR18

2:58pm April 17th 2018 via Hootsuite

A2: Lo: #AACR18 More biology - what's the clearance mechanism? A DNAse KO mouse - didn't change the profile of cfDNA size.

2:57pm April 17th 2018 via Hootsuite

Q: Moving to cancer screening, what else needs to be solved? Lo: #AACR18 Concentration so low, will need to look at many angles. (Holistic)

2:56pm April 17th 2018 via Hootsuite

Q: qPCR vs NGS? Lo: #AACR18 Their limit of det is 20 copies/mL for qPCR. If above that, will go onto NGS.

2:54pm April 17th 2018 via Hootsuite

Lo: #AACR18 PPV 2x the NEJM reported value (19.5%) with only 1x time point testing. Concl: EBV shows potential for early cancer screening

2:53pm April 17th 2018 via Hootsuite

Lo: #AACR18 Shows ability to do a training and test set, and can discriminate on both size of cfDNA and amount. Modeled back to 20K subjects

2:51pm April 17th 2018 via Hootsuite

Lo: #AACR18 Deletions of entire p arm, ampl of entire q arm, will affect size distribution as well.

2:48pm April 17th 2018 via Hootsuite

Lo: #AACR18 Shows fig from '10 STM https://t.co/ppV6VTqPvp fetal cfDNA has 20bp difference, two turns of the helix

2:46pm April 17th 2018 via Hootsuite

Lo: #AACR18 Logistics: 4w pause between tests, individuals may get worried. Lays out plan to do a 2-in-1 method (asked not to post)

2:43pm April 17th 2018 via Hootsuite

Lo: #AACR18 Older - higher risk. Temp of the day blood collected (!) Lower temp - higher FP rate. 'Summer rate 1/2 of winter'

2:40pm April 17th 2018 via Hootsuite

Lo: #AACR18 Their NEJM paper '17 https://t.co/mH9TxfYT5w 50 EBV copies/cell. FP's is likely due to viral re-activation. Predisposition?

2:39pm April 17th 2018 via Hootsuite

Lo: #AACR18 Able to get PPV of 11%. 75% of individ III/IV. NPC ID'd by screening. PFS hazard ratio improved 10x

2:38pm April 17th 2018 via Hootsuite

Lo: #AACR18 Could reduce FP rate, nasal endoscopy and MRI. Weekly commun. visits, 200 indiv/week. 20,174 subjects, 5.5% pos, 1.5% double pos

2:38pm April 17th 2018 via Hootsuite

Lo: #AACR18 cfDNA is an archetype - to answer early detection question. Plasma EBV: at beginning everyone tested for EBV. If + retest in 4w

2:36pm April 17th 2018 via Hootsuite

Lo: #AACR18 cfDNA has a half-life of 4d. After surgery - 1.5h Clin monitoring - reflect what happens to tumor.

2:35pm April 17th 2018 via Hootsuite

Lo: 1:39 risk of NPC (Nasopharyngeal Cancer) among southern Chinese. EBV in the blood - '99 https://t.co/Sn1NMeh1DA #AACR18

2:33pm April 17th 2018 via Hootsuite

Y. M. Dennis Lo (Univ HK) "Towards cancer screening using circulating DNA" #AACR18

2:31pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 Program in 2w: https://t.co/LrbDyQfp1H and two post-docs open.

2:28pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 (Shows unpublished data.) Other circulating, complementary info from ctDNA, cfmiRNA, exosomes. CANCER-ID

2:27pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 No spec morphology to initiate metastasis Shows hierarchical clustering of 9 lines; they differ

2:24pm April 17th 2018 via Hootsuite

Alix-Panabieres: Need at least 300 CellSearch cells, to get cells to grow. Many events to get a rare event for metastatic seeding #AACR18

2:23pm April 17th 2018 via Hootsuite

Alix-Panabieres: Their CTC-derived line was stable now for 5y. '17 https://t.co/gvimww5DOq a 'molecular portrait' Spec transcription #AACR18

2:22pm April 17th 2018 via Hootsuite

Alix-Panabieres: Capture of CTCs from colon cancer pts from their liver '15 https://t.co/A5wWJNMQPd Phenotypes, scRNA-seq #AACR18

2:20pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 First to culture CTCs https://t.co/mP2y6a8X2W won an #AACR17 award for this work.

2:19pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 Points to this '14 Lancet Oncol paper https://t.co/fxnm2ErCR4 regarding clinical validity of CTC

2:18pm April 17th 2018 via Hootsuite

Alix-Panabieres: >60% of Br Ca pts had CTC's expr PL-L1. Q: were enough cells analyzed? Still too early for screening, early det #AACR18

2:16pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 They were the first in '15 to show PD-L1 expression on Br Ca CTCs. Ref https://t.co/xbodEFvB4C

2:13pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 Fig from '17 ref https://t.co/WPYAMyohJ1 Points out DEPArray system for single CTCs w/Silicon Biosystems platform

2:12pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 Biology - CTC resistant to 'Anoikis apoptosis'. EMT, invasion or intravasation. CTCs can be molecularly char.

2:11pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 EpCAM+/-, shows staining within the drops. Now validating in Pr Ca pts, called EpiDrop technique, Cp w/CellSearch

2:10pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 Can look at PSA molecules/sec excretion (for example from LNCaP cell line) Shows heterogeneity of excretion

2:09pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 Based on what cells can shed for viability. '17 ref https://t.co/2GHZA1t1po Can use microdroplet encapsulation

2:08pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 Reviews fig from '14 https://t.co/DqheLOsImP Impt to target functional CTCs - viable based on short-term culture

2:06pm April 17th 2018 via Hootsuite

Alix-Panabieres: #AACR18 Real-time liquid biopsy - '13 Clin Chem https://t.co/n3ZGDgwlC8 CTC enrichment based on biology or physical props

2:05pm April 17th 2018 via Hootsuite