A picture is worth a thousand bugs – microBEnet: the microbiology of the Built Environment network. https://t.co/POlWR33W8v
4:45pm February 14th 2016 via Hootsuite
The chips are down for Moore’s law : Nature News https://t.co/NVJfD6jn8l
3:05pm February 14th 2016 via Hootsuite
RT @AnthonyBEarles: #SciLaughs Mr. Schrodinger? It's about your cat. I have good news or bad news.
1:45pm February 14th 2016 via Hootsuite
How Rare is a Rare Disease? | ASU Center for Evolution & Medicine https://t.co/QIn9J88Y5H
12:10pm February 14th 2016 via Hootsuite
CoreGenomics: #AGBT16 day 4: a new sequencing chemistry from Nanostring https://t.co/P2nIPPqwNl Neat stuff!
11:05am February 14th 2016 via Hootsuite
#AGBT16 great to see @becky_kusko @CIgenomics @claritas4kids @genomebiology @bioinformagician @pathogenomenick & friends at @RocheSequen
10:05am February 14th 2016 via Hootsuite
Disparity in Life Spans of the Rich and the Poor Is Growing - The New York Times https://t.co/OOsu23B3bL
9:45am February 14th 2016 via Hootsuite
Human Complex Trait Genetics in the 21st Century | Genetics https://t.co/savk8Z2G3K
7:31am February 14th 2016 via Hootsuite
RT @BBCWorld: The pigeon will see you now https://t.co/NJcvu5FEgS
4:30am February 14th 2016 via Hootsuite
RT @despair: The “Windows” section of every open-source project’s “how to compile and install” file: https://t.co/JRycpFvx4J
10:50pm February 13th 2016 via Hootsuite
RT @FastCoExist: Watch your back, technology is on verge of putting many office jobs at risk https://t.co/wCjZ4vB2pJ https://t.co/gYNRhzLqpY
9:35pm February 13th 2016 via Hootsuite
RT @SynBioBeta: Homebrew Biology Kit Brings #SyntheticBiology to the Masses https://t.co/o59vse0164 #synbio https://t.co/xvjGzvxWko
8:30pm February 13th 2016 via Hootsuite
Shenzhen, one of the most exciting startup hubs on the planet - WSJ https://t.co/zaQqwWFNMf
7:20pm February 13th 2016 via Hootsuite
.@OmicsOmicsBlog Alas the date of #agbt17 wasn’t announced at the end of #AGBT16, will provide that feedback.
6:32pm February 13th 2016 via Hootsuite in reply to OmicsOmicsBlog
.@GenomeNathan Perhaps @pacbio can answer - Jonas spent time w/Bobby Sebra’s data to show Sequel equal, and then exon splice var’s diff?
5:49pm February 13th 2016 via Hootsuite in reply to GenomeNathan
RT @bioinfomagician: Despite not being at Marco Island this year, had a great time and #AGBT16 remains one of my favorite meetings. Look fo…
5:11pm February 13th 2016 via Hootsuite
.@iGenomics I did make the correction. (Hey I'll just delete the incorrect tweet...)
4:23pm February 13th 2016 via Hootsuite in reply to iGenomics
Comment on poster session: many weren't up, could have been handled better. #AGBT16
4:22pm February 13th 2016 via Hootsuite
Precision Health: wanted to not squeeze out the genomic tech focus; still want to seed the Sept mtg, but a focus on medical apps #AGBT16
4:20pm February 13th 2016 via Hootsuite
#AGBT16 AGBT Precision Health will continue the same theme, closer to the clinic. Sept 22-24 Committed for several years.
4:19pm February 13th 2016 via Hootsuite
My three #AGBT16 blog posts: https://t.co/3KTWbncPfs https://t.co/2yK3qkDGmy and https://t.co/VLGk0caIWq Thank you everyone!
4:13pm February 13th 2016 via Hootsuite
Hill: 86% of indels (>50bp) variants were not previously observed. Shows large 130kb inversion prev unknown #AGBT16
4:10pm February 13th 2016 via Hootsuite
Hill: Added megabases of missing exon and regulatory data. #AGBT16
4:07pm February 13th 2016 via Hootsuite
Hill:Susie3 closes 94% of gorGor3 gaps. Added 164MB of euchromatic sequence. #AGBT16
Hill: Detecting mis-assemblies: looking for spikes in depth of coverage. BAC and fosmids coverage is the back-up supporting info #AGBT16
4:06pm February 13th 2016 via Hootsuite
Hill: New assembly: an 800-fold improvement in contig size. 23Mb N50 scaffold, 9.6Mb contig N50. Contigs reduced from 464K to 16K #AGBT16
4:04pm February 13th 2016 via Hootsuite
Hill: (Oops, meant he credited @infoecho ) #AGBT16
4:03pm February 13th 2016 via Hootsuite
Hill: Credits @igenomics Using BACs to scaffold contigs. The 12 prior assembly method reviewed https://t.co/9UCu95kPle #AGBT16
Hill: SMRT seq: error rate, long high-quality DNA needed. Goal to use PacBio. #AGBT16
4:01pm February 13th 2016 via Hootsuite
Hill: Problems: repetitive seq, SINEs and Alus, LINES, L1 and segmental duplications. Nice summary of Sanger and NGS #AGBT16
4:00pm February 13th 2016 via Hootsuite
Hill: (Thought he'd make a joke showing photo of Venter on the same slide as apes, but he didn't go there.) #AGBT16
3:59pm February 13th 2016 via Hootsuite
Hill: Showed great ape lineage tree and assemblies. Orangutan, gorilla, chimp, bonobo. #AGBT16
Hill: He has no disclosures. But is happy if you'd like to add to the list. (Showed a blank screen, then a tumbleweed.) #AGBT16
3:58pm February 13th 2016 via Hootsuite
Christopher Hill (Univ Washington) Long-read sequence assembly of the gorilla genome #AGBT16
3:57pm February 13th 2016 via Hootsuite
Biezuner: Case study in AML, work via prior platform. Ref '12 https://t.co/SFpFkEYp4L Showed prelim data to recapitulate #AGBT16
3:53pm February 13th 2016 via Hootsuite
Biezuner: Validated by DU145 cell-line (prostate ca), 15 divisions per clone; single cells and regrown; every time sampled cells #AGBT16
3:48pm February 13th 2016 via Hootsuite
Biezuner: Shows video of Tecan liquid handling automation; also uses Echo550 acoustic liquidhandler (inverted 1536-well plate) 2nL #AGBT16
3:46pm February 13th 2016 via Hootsuite
Biezuner: Single cell WGA, use 1st PCR on Access-array. 2nd PCR do barcoding of 48 cells; pool and sequence. #AGBT16
3:45pm February 13th 2016 via Hootsuite
Biezuner:Current methods - not cost-effective nor scalable (WES at low AF) They use microsatellites and get cost to $40/cell #AGBT16
3:44pm February 13th 2016 via Hootsuite
Biezuner: Can reconstruct phylogenetic tree as mutations occur during development. #AGBT16
3:42pm February 13th 2016 via Hootsuite
Biezuner: '03 Sulston cell lineage and beyond in c elegans https://t.co/NuJ1m37Pv4 but in human? #AGBT16
3:41pm February 13th 2016 via Hootsuite
Biezuner: Humans are 10^13 cells. But in development - what happened from one zygote to adult. '13 Nat Rev https://t.co/kohbBBcOru #AGBT16
3:39pm February 13th 2016 via Hootsuite
Tamir Biezuner (Weizmann Institute of Science) A generic, cost-effective and scalable cell lineage analysis platform #AGBT16
3:37pm February 13th 2016 via Hootsuite
Korlach:Q:Roadmap for chem improvement? A:Sample prep same; chemistry will both be supported, improving both, analysis same #AGBT16
3:36pm February 13th 2016 via Hootsuite
Korlach:Q:6x loading but library amt the same? A:Vol is little more, amt dep on insert size. At work on improving loading still #AGBT16
3:35pm February 13th 2016 via Hootsuite
Korlach: Showing many isoforms ID's from Sequel not in RSII (NB - unclear to me why Sequel better in this application) #AGBT16
3:34pm February 13th 2016 via Hootsuite
Korlach: CRC line SW480 before/after siRNA for splicing machinery - cp RSII and Sequel - about 4x higher detect. Ex of exon skipping #AGBT16
3:33pm February 13th 2016 via Hootsuite
Korlach:Iso-seq - using Univ Human Ref RNA (UHRR) showing transcript isoform detection on Sequel. 3 genes, only 2 were det on RSII #AGBT16
3:32pm February 13th 2016 via Hootsuite
Korlach: Structural var in Glioblastoma (GBM) - also from workshop yesterday. #AGBT16 video here: https://t.co/6cG0ABtCdF
3:31pm February 13th 2016 via Hootsuite
Korlach: Challenging C9orf72 ALS G4C2 repeat mentioned yesterday - very difficult >900 bases of G's and C's (!) #AGBT16
3:29pm February 13th 2016 via Hootsuite
