GO:Marchantia - liverwort, is being looked at as another model, haploid, many attractive features #PAGXXIII
11:42am January 14th 2015 via Hootsuite
GO:We need this kind of throughput - as plants are so much more complex than bacteria used in synthetic biology. Biggest challenge #PAGXXIII
11:41am January 14th 2015 via Hootsuite
GO:Found barley most efficient transformation; 100's of constructs this year. Not a rapid cycle. Synth bio: 80k-100K #PAGXXIII
11:40am January 14th 2015 via Hootsuite
GO:Design, build, test, repeat: an engineering cycle. Uses GoldenGate cloning 2014 PLOS ref http://t.co/jSFfbSPUrU #PAGXXIII
11:39am January 14th 2015 via Hootsuite
GO:Looking at complementation of ccamk w/ constitutive promoters; finding which work best for which gene in legumes #PAGXXIII
11:37am January 14th 2015 via Hootsuite
GO: Engineering the NOD factor kinases upstream; downstream. 'Shown as straightforward' #PAGXXIII 2012 fig http://t.co/OzpU41nYJG
11:36am January 14th 2015 via Hootsuite
GO: Complex figure of activation shown: CCaMk+Cyclops+TF+RNAPol activates ERN1, NIN for nodulation. #PAGXXIII
11:33am January 14th 2015 via Hootsuite
GO:Downstream: have two gain-of-fn mutations (CCaMK and Cyclops) for nodulation formation. Complex map of symbiosis signalling #PAGXXIII
11:31am January 14th 2015 via Hootsuite
GO: Shows calcium spiking in rice from diff oligosaccharides. Legumes vs non-legumes: lyto-keto-oligosaccharide specificity #PAGXXIII
11:30am January 14th 2015 via Hootsuite
RT @YiliangDing: @giles oldroyd is giving a marvelous keynote talk at #PAGXXIII ----fix nitrogen in cereals!!! http://t.co/fEH4ipQoWE
11:27am January 14th 2015 via Hootsuite
GO:Simpler engineering problem: no need for calcium signalling. Cereals have it - just activate w/ Myc factor & downstream signals #PAGX
GO:Fungus at the root & can't get in. Legumes don't fn diff, just added on NOD function and later nodulation. Core conserved #PAGXXIII
11:26am January 14th 2015 via Hootsuite
GO:This myc signalling pathway at the base. Fig from 2002 TIPS ref http://t.co/MSvYTlGkvB Wheat have this pathway, unactivated #PAGXXIII
11:23am January 14th 2015 via Hootsuite
GO:Phylo tree of plant kingdom: nodulation 60MYA, but mycorrhizal about 450MYA, the earliest land plants. Thus myc everywhere #PAGXXIII
11:22am January 14th 2015 via Hootsuite
GO:Called symbiosis signalling pathway: both myc factor strigolactone (from microrrhyzo fungi) and NOD factors. #PAGXXIII
11:20am January 14th 2015 via Hootsuite
GO: 2006 Nature ref http://t.co/a0AFkeAuj8 engineered gain-of-function in CCaMK pathway, entire NOD pathway to coord. dev #PAGXXIII
11:19am January 14th 2015 via Hootsuite
GO:Ca2+ oscillations in root-hair shown - when NOD factor present. Transmitting NOD factor presence into nucleus #PAGXXIII
11:17am January 14th 2015 via Hootsuite
GO:Wt vs nsp2 mutant - can't make nodules. Assembled signal transduction pathway, for the NOD factor production. Movie root hair #PAGXXIII
GO:They are at step 1: rhizobial recognition into cereals. Steps 2-4 require more knowledge in legumes. Used forward genetics #PAGXXIII
11:16am January 14th 2015 via Hootsuite
GO:'The biggest challenge: engineering bacterial infection.' But there are simpler ways in other legumes. Env.: restrict O2 #PAGXXIII
11:15am January 14th 2015 via Hootsuite
GO:Lots of complex development, engineer in pieces. Steps: rhizobial recognition; nodule formation; bacterial inf.; environment #PAGXXIII
11:14am January 14th 2015 via Hootsuite
GO:NOD factors - showed structure from S. meliloti. Known for 25y, showed developmental process in root hair. Infection thread #PAGXXIII
11:12am January 14th 2015 via Hootsuite
GO:Legumes have to find the N2-fixing bacteria; flavenoids released from plant, NOD factors released from bacteria, nodules form #PAGXXIII
11:11am January 14th 2015 via Hootsuite
GO:Thus for small-holder famers: if 3-5kg goes to 25-50kg their yield will double or triple. Could also reduce fertilizer use #PAGXXIII
11:10am January 14th 2015 via Hootsuite
GO:Much simpler than pea: less efficient but still useful. Graph of kg ha^-1: ave 134kg max yield. Small-holders: only 3-5kg #PAGXXIII
11:09am January 14th 2015 via Hootsuite
GO:Legumes the prototype: self-fertilizing crops. A false assumption: need for nodule on a cereal root. Pea nodule the pinnacle #PAGXXIII
11:07am January 14th 2015 via Hootsuite
GO:Greatest potential is sub-Saharan Africa,. @gatesfoundation supported: Engineering the nitrogen symbiosis for Africa #PAGXXIII
11:06am January 14th 2015 via Hootsuite
GO:Thus: major limitation for maize is nutrients, not water. N2 and phosphorus. Too little in dev world; too much in 1st wrld #PAGXXIII
11:05am January 14th 2015 via Hootsuite
GO: Map of global consumption 2012 Mueller Nature ref http://t.co/zSmEgU7gZy Overlay with Maize-prod areas, nutrient-limited #PAGXXIII
11:03am January 14th 2015 via Hootsuite
GO:Solving the nitrogen problem - we are heavily dependent. N2 fertilizers causing nitrification, algal blooms #PAGXXIII
11:02am January 14th 2015 via Hootsuite
Last plenary: Giles Oldroyd (John Innes Centre UK) Engineering Nitrogen-Fixing Symbiotic Associations in Cereals #PAGXXIII
11:00am January 14th 2015 via Hootsuite
RT @SeqComplete: #PAGXXIII #genomics great tweetline contributors @klsywd @wheatgenome @DaleYuzuki @taylorlawley @mt_ur_mind @assemblathon
10:43am January 14th 2015 via Hootsuite
.@Sagebio Oops! Noted.
10:42am January 14th 2015 via Hootsuite in reply to
Over next weeks my updates from #PAGXXIII (both video and posts) will be live on @lifetech's Behind the Bench http://t.co/FUiRKO0eJh
10:41am January 14th 2015 via Hootsuite
RT @moorejh: Great Nature Reviews Genetics paper on data integration by @MarylynRitchie & @sap_PSU http://t.co/atzTMeD8BA #bioinformatic
10:31am January 14th 2015 via Hootsuite
RT @biomickwatson: Some details on the new HiSeq 3000 and 4000 http://t.co/M9a9ojeP6d
9:25am January 14th 2015 via Hootsuite
More at #AGBT15 MT @genetics_blog: 10X Genomics Raises $55.5M. Seems to be a hack on ILMN machine to get long reads. http://t.co/dmPVEdnS5F
8:20am January 14th 2015 via Hootsuite
RT @mclafave: http://t.co/rUj5CDsSvv now has notes from the #PAGXXIII @PacBio workshop. ~50x PacBio seq gives you a pretty solid assembly.
7:45am January 14th 2015 via Hootsuite
MT @moorejh: ID'ing & amending mislabeled #DNA sequences in #genomics DB's - The Scientist http://t.co/sEY1Fg2TgP http://t.co/XK3ZxVDZMW
7:20am January 14th 2015 via Hootsuite
RT @washingtonpost: Map: Literally every goat in the United States http://t.co/jgYdOtuxFV http://t.co/1dx8qsYiGh
6:15am January 14th 2015 via Hootsuite
RT @KAMChapman: Wonderful - sometimes a speech goes even further than even a paper. http://t.co/eQATQl0TPp @npch #Force2015
5:45am January 14th 2015 via Hootsuite
RT @globeandmail: ICYMI: Scientists unleash the power of immunotherapy on stubborn cancers http://t.co/aiWOwgFGJT
4:15am January 14th 2015 via Hootsuite
RT @SMToolsGo: How to Power-Up OS X Spotlight with Flashlight via @lifehacker http://t.co/hSUN1c1kfU
3:05am January 14th 2015 via Hootsuite
RT @lexnederbragt: The PromethION as 'the Borg version of the MinION' http://t.co/W1g0kC0Sk0 Nicely done by @OmicsOmicsBlog #nanopore
2:01am January 14th 2015 via Hootsuite
RT @humanlongevity: Personal Genome Diagnostics, Inc. and Human Longevity, Inc. Sign Cancer Genomics Agreement http://t.co/bNId7DbHDg
1:05am January 14th 2015 via Hootsuite
Woman recreates portraits of her ancestors going back 200 years | i100 http://t.co/SrXU00BEt3
12:20am January 14th 2015 via Hootsuite
RT @cbtadvisors: Look out, @Organovo MT @3dersorg OxSyBio raises £1m 3D printer 4synth tissue http://t.co/FfFLVCEL4z http://t.co/TFgcftOMoH
11:50pm January 13th 2015 via Hootsuite
RT @AstroTerry: #Astrobutch took this early this morning- Mid Atlantic USA with #aurora and #sunrise- wow! http://t.co/e0baMWi2eq
10:40pm January 13th 2015 via Hootsuite
NT:New trials established for TME14. Acknowledges @gatesfoundation #PAGXXIII
8:45pm January 13th 2015 via Hootsuite
NT:But most plants also affected by Cassava Mosaic Disease - now acquired susceptibility. CMD2 appears turned off (!) #PAGXXIII
8:43pm January 13th 2015 via Hootsuite
