RT @willrich45: "What is good screen time?" http://t.co/mkp5Z5qCjq Interesting. #edchat #education
7:15am October 1st 2014 via Hootsuite
Hacking the Cancer Genome - IEEE Spectrum http://t.co/95lxF6igwe
5:05am October 1st 2014 via Hootsuite
A 15-Step Guide To Understanding Why Hong Kong Has Erupted In Protest | Buzzfeed http://t.co/DwaO0dDTTK
4:05am October 1st 2014 via Hootsuite
MT @IcahnMountSinai: “We don’t know the language yet of #biology." Eric Schadt @IcahnInstitute discusses http://t.co/njWHh586Tl #biotech
10:05pm September 30th 2014 via Hootsuite
BRAIN: Launching America’s Next Moonshot | NIH Director's Blog http://t.co/gaL6EXVEy3 $46M awarded
9:00pm September 30th 2014 via Hootsuite
Why I'm returning my iPhone 6 (well, maybe) | Macworld http://t.co/WQKdC0abLl Agree with this, seeing it in-use today (co-workers').
8:00pm September 30th 2014 via Hootsuite
How to double your free OneDrive storage to 30GB | PCWorld http://t.co/DAMwT2YSnK Before Oct 1st!
7:05pm September 30th 2014 via Hootsuite
Personal genomics: The test for everything (with video) | Vancouver Sun http://t.co/xsLznP6u4V
6:00pm September 30th 2014 via Hootsuite
CDC Confirms First Case of Ebola in U.S. - WSJ http://t.co/6w5M0qAOhQ
5:36pm September 30th 2014 via Hootsuite
RT @NewsfromScience: Just how big is Google Scholar? http://t.co/B0XnDu5SGU
5:01pm September 30th 2014 via Hootsuite
To repeat "Don't try this at home" RT @michaelpollan: Jeff Leach gets fecal transplant from a hunter gatherer. http://t.co/Q2q2fGbcmT
4:00pm September 30th 2014 via Hootsuite
RT @moorejh: Our United Genomes Project http://t.co/9DuJ7Xf5WQ #genomics #africa
3:03pm September 30th 2014 via Hootsuite
Broeckel: Dose-response, chronic stimulation, shows phenotypic variation in fold-changes of specific genes #gensoltour
1:48pm September 30th 2014 via Hootsuite
Broeckel: Developed a production phenotyping workflow: each (250 lines) stimulated, RNA and protein harvested, qPCR screen #gensoltour
Broeckel: Comparing iPSC-derived cardiomyocytes with biopsy samples, comfortable with the cell model #gensoltour
Broeckel: Stimulated with inducers of hypertrophy, did gene exp. analysis and PCA; nice separation #gensoltour
1:47pm September 30th 2014 via Hootsuite
Broeckel: Need is for a better model. NHLBI's HyperGen; description here http://t.co/1G7o35d1C0 250 iPSC lines they produced #gensoltour
Broeckel: Left VH: indep CV risk factor, inter-individ. var., "hypertensive & diabetic heart disease" #gensoltour
Broeckel: ...to functional 'what the genome is doing'. Left ventricular hypertrophy, increase in mass of left side of heart #gensoltour
Broeckel: Begins with a 'Congratulations!' slide - advances in genetics / genomics. It's time to move from statistical ass'n... #gensoltour
1:46pm September 30th 2014 via Hootsuite
Next: Ulrich Boeckel, Medical Coll WI "RNA Sequencing research applications in human induced pluripotent stem cell tissues" #gensoltour
O’Connor:Q:Other protocols for 10K cells? Comment? A:May not be realistic for their work on clinical research samples #gensoltour
O’Connor:A: There are some 50 epigenetic-based drugs in various phases of testing #gensoltour
O’Connor:Q:How far along for drug dev.? A:Emphasizes the epigenetic component for disease, and for populations #gensoltour
1:45pm September 30th 2014 via Hootsuite
O’Connor: Being able to look at env. effects on phenotype, then examine via ChIP. Found Th1 protein. Potential v. exciting #gensoltour
O’Connor: Looking at T-cell activity, looking at STAT3, 4, FOXP3 genes and H3K27me3 peaks (repressive mark) #gensoltour
O’Connor: Also looking at a lung disease Sarcoidosis BAL, look at epigenetics of CD4+ T-cells. BAL: lavage of bronchus #gensoltour
O’Connor: In future: look at known asthma pathways with epigenetic inhibitors, look at changes in phenotype #gensoltour
O’Connor: Called out IRF4 transcription factor consistency; listed top hits, referred to Lugt et al 2014 Nature Immunol #gensoltour
O’Connor: Complex Venn: vast majority common across 5M, 3M (>92%) 1M (80%), their Q>=20 #gensoltour
O’Connor: Conf. (log-scale) values dropped from 32 (initially) now to 14 at 3M, 5M cells; 1M now at 17 'x-value' #gensoltour
O’Connor: Looking at peak overlap and p-values (confidence) w/different samples: 1M may need slightly higher confidence #gensoltour
1:44pm September 30th 2014 via Hootsuite
O’Connor: Will be helping @iontorrent produce a ChIP-Seq application note soon. Run report down to 1M cells shown - reliable #gensoltour
O’Connor: Needed to expand cells, dendritic cell subtypes; fixate first, then flow sort. Cp 1M, 3M, 5M, 20M cells #gensoltour
O’Connor: Some peaks may be called but not replicate. Low input: 10^5 cells, first expt's 0 sites. Hi input: 106K sites #gensoltour
O’Connor: Also low cost/run; been able to do ChIP-Seq with hard-to-get samples. Reviewed concept of peak-calling #gensoltour
O’Connor: Hard to get samples: ChIP-Seq typically uses 10-20 10^6 cells. PGM / Proton 'we love them b/c they are really quick' #gensoltour
O’Connor: Pro-allergy CD11b+ DC; viral protecting CD103+ DC; epigenetic fig. from 2006 pub http://t.co/NuwfamiWBL #gensoltour
O’Connor: Asthma increasing to 25.7M in US (CDC), 7M under 18; unk. etiology. Focus: epigenetic potential of marrow stem cells #gensoltour
First up: Brian O'Connor, Nat'l Jewish Health (Denver) "Epigenomics & disease research: optimizing Ion Proton ChIP-Seq" #gensoltour
1:43pm September 30th 2014 via Hootsuite
Hello world- next set of 117 tweets are from yesterday's Genetic Solutions Tour (#gensoltour) where I was tweeting under @iontorrent. Enjoy!
RT @Immunogenomics: Find info, abstracts, schedule, speaker bios and more at http://t.co/d37TvFK1tM #IGC2014
1:31pm September 30th 2014 via Hootsuite
Bibby Scientific, PCRmax Breathe New Life into Former Illumina qPCR System| GenomeWeb http://t.co/DE3mVvzSQv
12:27pm September 30th 2014 via Hootsuite
RT @genome_gov: Thurs/Fri tune into NHGRI’s live webcast of GM7 meeting: Genomic Clinical Decision Support #GCDS2014 http://t.co/6A4BCV1bYT
11:03am September 30th 2014 via Hootsuite in reply to
RT @iontorrent: Congrats to Stephen Scherer of @SickKidsNews on being named as potential @NobelPrize recipient! http://t.co/0nZtQgQacL
9:45am September 30th 2014 via Hootsuite
RT @UnlockLifesCode: Genome Closing Symposium Today at 1pm @NMNH http://t.co/Rl5uBG2XXg Eric Green w @annewoj23 + Richard Lifton.
9:32am September 30th 2014 via Hootsuite
Finding Risks, Not Answers, in Gene Tests - NYTimes http://t.co/mPf3y9A7RR
7:00pm September 29th 2014 via Hootsuite
New post from Behind the Bench: Ion PGM™ Dx System listed with the U.S. FDA as a Class II Medical Device http://t.co/OPgafvS6Sl
2:29pm September 29th 2014 via Hootsuite
RT @verge: Can 1Password save your digital life? http://t.co/TL9o3a7gBR by @hamburger
12:05pm September 29th 2014 via Hootsuite
RT @scicurious: Tearing down that pesky 10,000 hour rule by Gladwell (of course). It's more than just practice. http://t.co/HmCOetnXBC
11:05am September 29th 2014 via Hootsuite
