Albers: 2013 function of FlaI, assembly of the two functions first an assy ATP-ase as well as a rotary one http://t.co/pv3Hlv5cBC #ASM2014
9:41am May 18th 2014 via Hootsuite
Albers: 2014 paper showing rotation of the archaellum: http://t.co/edBEUlSuqL #ASM2014
9:39am May 18th 2014 via Hootsuite
Albers: Movies comparing Brownian motion vs. rotating archaellum 2012 Lassak publ.: http://t.co/ciSc4VVj3U #ASM2014
9:38am May 18th 2014 via Hootsuite
Albers: Imp. for surface adhesion, biofilm formation, strain-spec DNA exchange system, and surface motility #ASM2014
9:36am May 18th 2014 via Hootsuite
Albers: S. acidocaldarius (S.a.) has three surface structures archaellum, 'threads', 'Aap pili'; showed cartoon of assembly #ASM2014
9:35am May 18th 2014 via Hootsuite
Albers: Archaellum - not related to flagellum at all. Well-conserved, Model: Sulfolobus acidocaldarius from Yellowstone crenarchaea #ASM2014
9:34am May 18th 2014 via Hootsuite
Albers: Figure 2012 review compares of the flagellum, archaellum and type IV pili http://t.co/2k6oqjqgsd pili serve to bind surface #ASM2014
9:32am May 18th 2014 via Hootsuite
Albers: 1991 publication Marwan et al J Bacteriol http://t.co/9UfRAA4x33 showed movie of movement. #ASM2014
9:31am May 18th 2014 via Hootsuite
Sonja Albers (Max Planck Inst. for Terrestrial Microbiolology, moving to Freiburg): “The Third Way to Move: How Archaea Swim” #ASM2014
9:28am May 18th 2014 via Hootsuite
Santangelo: Shown to block translation; likely able to 'move to all genes'; several done so far #ASM2014
9:23am May 18th 2014 via Hootsuite
Santangelo: Able to tune expression via a functional 'riboswitch'. 85nt 5' UTR binding flouride blocking xcr or xl #ASM2014
RT @ASMicrobiology: Hey #asm2014 we are so popular we are being hashtag spammed; we are working on it! Help us block the spammers. ^lp
9:20am May 18th 2014 via Hootsuite
Santangelo: Some portions are nucleosome free: on the ribosomal operon, may be due to pace of movement; 40min repl. rate #ASM2014
9:19am May 18th 2014 via Hootsuite
Santangelo: Was able to recapitulate pattern in-vitro, has histones and get same patterns. Showed promoters histone-free #ASM2014
9:18am May 18th 2014 via Hootsuite
Santangelo: T. Kodakarensis digestion of chromatin, ~60bp fragments 2013 publication: http://t.co/Y68iGpcxs0 #ASM2014
9:16am May 18th 2014 via Hootsuite
Santangelo: Archaeal transcription machinery: nucleosomes affect it; working on 3D structure #ASM2014
9:15am May 18th 2014 via Hootsuite
.@ilyachorny I reported the ~6 spammers, my feed now cleaned up. #ASM2014
9:13am May 18th 2014 via Hootsuite in reply to
Santangelo: Movie of clamp and jaw - move in synchrony. Rotation out of the way, universal movement (conserved hinge region) #ASM2014
9:11am May 18th 2014 via Hootsuite
Santangelo: Generated 75 mutants; now looking at motions of RNAP: jaw/lobe, swinging clamp, swinging stalk #ASM2014
9:10am May 18th 2014 via Hootsuite
Santangelo: Comparing Eury, Cren, Euk. Pol.I and Pol.II: uniqueness of T. kodakarensis, the simplest of the 4 #ASM2014
9:09am May 18th 2014 via Hootsuite
Santangelo: Thermococcus kodakarensis RNAP: able to get crystal structure, distinct open and closed states #ASM2014
9:08am May 18th 2014 via Hootsuite
Santangelo: Showing figure from 2009 Review Hirata Murakami http://t.co/tEGxP9AkUD about RNA polymerase and their relatedness #ASM2014
9:06am May 18th 2014 via Hootsuite
Next: Thomas Santangelo (Colorado State) "Regulated Archaeal Transcription" #ASM2014
9:00am May 18th 2014 via Hootsuite
Schleper: Finished w/ a figure from her review PubMed http://t.co/qXtCfSIYRu #ASM2014
8:59am May 18th 2014 via Hootsuite
Schleper: Bovine methanogen paper Poulsen 2013 Nature Comm PubMed: http://t.co/GNqGOwBbdD #ASM2014
8:58am May 18th 2014 via Hootsuite
Schleper: Perhaps Archaebiotics could reduce trimethylamine in vivo? #ASM2014
8:56am May 18th 2014 via Hootsuite
Schleper: Cows with rapeseed oil, transcriptomics made it clear the RCC cluster reduced methane#ASM2014
8:53am May 18th 2014 via Hootsuite
Schleper: Looking at mitigation in rumenants: methanobaceriales, also Thermoplamata looked at more closely #ASM2014
8:52am May 18th 2014 via Hootsuite
Schleper: 7th order of methanogens - started in bovine rumen. 17% of global methane output #ASM2014
Schleper: On the skin: 2013 PLOS paper http://t.co/wsTIJgup2X how could these have been overlooked in the HMP? #ASM2014
8:51am May 18th 2014 via Hootsuite
Schleper: Acidic peat evidence of same mechanism at work in recent Nature Geosci paper http://t.co/7O53NLAdKe #ASM2014
8:50am May 18th 2014 via Hootsuite
Schleper: De-convoluting the mechanism: NO2- and NH4+ contribute equally to N2O production under hi/lo O2; hybrid formaton likely #ASM2014
8:48am May 18th 2014 via Hootsuite
Schleper: Archaea also produce N2O, shown from soil microorg's, different org's produce under different O2 conditions (hi/low) #ASM2014
8:47am May 18th 2014 via Hootsuite
Schleper: Human impact on the N-cycle: chemical fixation by the Haber-Bosch process to prod fertilizer. #ASM2014
8:45am May 18th 2014 via Hootsuite
Schleper: Figure from http://t.co/Qms5Vtn6uf review on phylogeny of ammonia oxidizing archaea; enrichments & pure cultures #ASM2014
8:41am May 18th 2014 via Hootsuite
Schleper: Even found in clean rooms at NASA, wastewater plants, on leaves #ASM2014
8:39am May 18th 2014 via Hootsuite
Schleper: Ammonia oxidation important in the nitrogen cycle, now 'massively studied' Thaumarchaeota found all over #ASM2014
8:38am May 18th 2014 via Hootsuite
Schleper: Not so many model org's for coastal marine plantktonic archaea. Thaumarchaeota, in 2005 Cenarchaeum ammonium oxidation #ASM2014
8:37am May 18th 2014 via Hootsuite
Schleper: Photo of bachelor students collecting hot spring samples and going to HT sequencing for analysis #ASM2014
8:35am May 18th 2014 via Hootsuite
Schleper: Many trained in southern Germany; photo in 2005 Bavaria - going outside E. coli #ASM2014
Schleper: Discovering unusual life forms, rule breakers, and dispelling dobmas. 100C, extremely low pH, virus changing shape #ASM2014
8:34am May 18th 2014 via Hootsuite
Schleper: RNA polymerases from Archaea isolated from Iceland - where it all began. "I found it rather boring" when first began work #ASM2014
8:32am May 18th 2014 via Hootsuite
Schleper: Two Germans inspired by Woese: Zillig (Munich) and Stetter (Regensburg) the 'first generation' #ASM2014
8:31am May 18th 2014 via Hootsuite
Schleper: "No one could imagine that there could be anything microbial that was not phylogenetically a 'eubacteria'" Woese #ASM2014
8:29am May 18th 2014 via Hootsuite
Schleper: Has been working with Archaea for 25y. From communities, sulfobales, CRISPR-virus defens, methanogens... #ASM2014
Up next: Christa Schleper, Univ Vienna, "The Archaea Among Us: Thaumarchaeota and Others that Affect Our Daily Life" #ASM2014
8:27am May 18th 2014 via Hootsuite
Pace: 'Heterotachy' is a variable rate of change among sequences "a statistical killer" causing problems with phylogeny #ASM2014
8:24am May 18th 2014 via Hootsuite
Pace: Crenarchaeota, Euryarchaeota 'split' goes deep; different opposing models Lake 1988 'Eocyte' model; Embley 2008 model #ASM2014
8:17am May 18th 2014 via Hootsuite
Pace: Found 3 domains but 'no root' until 1989 and PNAS 1990 PubMed http://t.co/zY1DC4prK0 divided Archaea into 2 #ASM2014
8:15am May 18th 2014 via Hootsuite
Pace: 'six-mer or longer' has low random probabilty: used as homologues, common ancestry, infer relationships. 16S rRNA #ASM2014
8:13am May 18th 2014 via Hootsuite
