RT @AppliedBio: Good Morning Boston and #ASHG2013 Let’s do lunch today: Room 259AB Level 2 We’ll talk qPCR & Digital PCR
6:38am October 23rd 2013 via Hootsuite
RT @iontorrent: Mark your #ASHG2013 maps, @LIFECorporation at booth #401 this year, @iontorrent bus at booth #100 http://t.co/x64qnl5rha
5:40am October 23rd 2013 via Hootsuite
RT @RayBiotech: More evidence that coffee is a 'wonder drug': Drinking Coffee Cuts Liver Cancer Risk by 40%: http://t.co/tkHM0nFcQH
4:41am October 23rd 2013 via Hootsuite
RT @novogenmexico: We congratulate everyone at #IonWorld2013 #Boston for organizing a great event! See you next year! @iontorrent
9:40pm October 22nd 2013 via Hootsuite
@AllSeq Is it good enough to say 'stay tuned for my blog post'? :o) Will try to work on it tonight... J/K - EA Mar 2014; planned May '14.
9:04pm October 22nd 2013 via Hootsuite in reply to
Oxford Nanopore's booth at the #ASHG2013 meeting in Boston. http://t.co/UpHdvXsOzN
8:25pm October 22nd 2013 via Hootsuite
TIL that a family in Sweden gets 350 paternity / maternity days off from work that is valid until the kid is 8 y.o., on top of 7w vacation
7:25pm October 22nd 2013 via Hootsuite
Reich: 'In contrast, no evidence of hybrid sterility in present-day humans'. (European ancestry in 30K African-Americans) #ASHG2013
7:18pm October 22nd 2013 via Hootsuite
Reich: X-chr. - esp. strong selection against Nean'l. From flies - 'the large X effect'; possible explanation. #ASHG2013
7:16pm October 22nd 2013 via Hootsuite
Reich: These deserts are gene rich, functional, selection to remove Nean'l ancestry. 10Mb large - 'removed quickly' #ASHG2013
7:15pm October 22nd 2013 via Hootsuite
Reich: "Most striking: the Neanderthal ancestry 'deserts'" 10Mb large, not by chance. #ASHG2013
Reich: Mapping back to East Asian individual (CHB HapMap), Cp to Europeans. #ASHG2013
7:13pm October 22nd 2013 via Hootsuite
Reich: Now trace back common risk factors from Neanderthals. (T1D in Latinos) #ASHG2013
Reich: About five different groups flow of genes into modern humans. 2% Neandertal introduce many new variants unseen before. #ASHG2013
7:12pm October 22nd 2013 via Hootsuite
Reich: Neandertal gene flow into Denisovan. 'Den's have ancestry from an unk. archaic population unrelated to Nean's' #ASHG2013
7:03pm October 22nd 2013 via Hootsuite
Reich: Altai highly inbred. 18Mb of homozygosity (!) "Huge chunks" of shared ancestry, 1/8th the genome shared. #ASHG2013
7:02pm October 22nd 2013 via Hootsuite
Reich: Population differences: 1/3 or 1/6 the heterozygosity. Diversity of population - among smallest of any organism. #ASHG2013
7:01pm October 22nd 2013 via Hootsuite
Reich: Same cave, toe bone, 'Altai Neandertal'. Denisova - in-between modern human and Neandertal. #ASHG2013
7:00pm October 22nd 2013 via Hootsuite
Reich: Neandertals 30-230kya. Modern humans <200kya. 'Magical Denisova cave' - a distal finger bone from a girl. #ASHG2013
6:59pm October 22nd 2013 via Hootsuite
David Reich, HMS: Population history from a high-coverage Neandertal genome. #ASHG2013
6:58pm October 22nd 2013 via Hootsuite
Lawrence: XIST cDNA removed complicating regularity elements. 'XIST alone is sufficient for chrom. silencing' #ASHG2013
6:56pm October 22nd 2013 via Hootsuite
Lawrence: Comment from audience: "Just beautiful" #ASHG2013
6:54pm October 22nd 2013 via Hootsuite
Lawrence: As a result of trisomy silencing, a volcano plot looking at genes / pathways changing most. (FDR vs. FC) #ASHG2013
6:51pm October 22nd 2013 via Hootsuite
Lawrence: Inducible system, both iso-genic and iso-epigenetic. XIST 5d later: proliferation; neural progenitor cells form rosettes #ASHG2013
6:49pm October 22nd 2013 via Hootsuite
Lawrence: Created an inducible 'disease in a dish' model, iPS cells due to pluripotency. Can use a drug screen to mimic reversal #ASHG2013
6:46pm October 22nd 2013 via Hootsuite
Lawrence: Looked at methylation analysis across Chr21. 'First convergence of 2 broad fields'. Chrom. regulation / pathology #ASHG2013
Lawrence: SNP analysis also showed this; microarray showed gene exp. change per chromosome #ASHG2013
6:43pm October 22nd 2013 via Hootsuite
Lawrence: FISH used to assess transcription foci. Nature reference: http://t.co/ZySROtGYPZ #ASHG2013
6:42pm October 22nd 2013 via Hootsuite
Lawrence: XIST into Chr21, got it to work. Jiang et al Nature Aug 2013. Silencing demonstrated via 8 methods. #ASHG2013
6:41pm October 22nd 2013 via Hootsuite
Lawrence: Chr21 SFN cost was $50K-$100K, done free by Sangamo. Done in iPS, could provide therapeutic cells. #ASHG2013
6:39pm October 22nd 2013 via Hootsuite
Lawrence: 'This won't work b/c...' but rec'd a high-risk NIH grant. First used ZFN to edit and paste 21kb transgene. #ASHG2013
6:38pm October 22nd 2013 via Hootsuite
Lawrence: Idea - take dosage imbalance from X-chrom inactivation. XIST is the 19kb non-coding RNA only from inactive X #ASHG2013
6:35pm October 22nd 2013 via Hootsuite
Lawrence: 'Chromosomal disorders remain largely outside hopeful advances of genetics and biomedicine' #ASHG2013
6:34pm October 22nd 2013 via Hootsuite
Lawrence: 1:140 births have a chrom. disorder. 1/700 T21; 400K in the US; 6M worldwide. 50% congen. heart disease #ASHG2013
6:33pm October 22nd 2013 via Hootsuite
Jeanne Lawrence 'Transl. dosage compensation to Trisomy 21' Translational epigenetics #ASHG2013
6:32pm October 22nd 2013 via Hootsuite
Useful! MT @girlscientist: If you don't want tweets from #ASHG2013 this week, here's one way to block a hashtag: http://t.co/IaoPO8jRQy
6:25pm October 22nd 2013 via Hootsuite
Loviglio: Correlated 49 differentially expressed genes in the deleted / dupl. regions. Of these 3 were correlated with ASD #ASHG2013
6:09pm October 22nd 2013 via Hootsuite
Loviglio: 5 genes in 600kb region selected for 4C analysis. 2 controls, 2 600kb del, 2 600kb dupl. #ASHG2013
6:02pm October 22nd 2013 via Hootsuite
Loviglio: Looked at region using 3C (chromosome conformation capture); higher-order chromosome organization affecting del. / dupl. #ASHG2013
6:00pm October 22nd 2013 via Hootsuite
Loviglio: 16p11.2 region proximal 600kb, microdeletion locus ass'd w/ autism, duplication w/ schiz. in addition to obesity. #ASHG2013
5:55pm October 22nd 2013 via Hootsuite
RT @Genomethics: DDD project aiming for 36,000 samples. Doing sequencing on trios #ASHG2013
5:46pm October 22nd 2013 via Hootsuite
RT @girlscientist: Don't forget #ASHG2013 tweetup tonight, 7:30pm, MJ O'Connor's Pub right next to the Westin.
4:25pm October 22nd 2013 via Hootsuite
RT @GeneticsSociety: Pack a coat for #ASHG2013! Highs only in the 50s this week; lows in the 30s. Forecast still calls for great science!
3:26pm October 22nd 2013 via Hootsuite
Mike McKenna, VP of Molecular Biology, wrapping up at #ionworld2013 http://t.co/HDpGUN6vqZ
2:31pm October 22nd 2013 via Hootsuite
McKenna: Showed 30Gb runs from a PII chip in October #ionworld2013
2:27pm October 22nd 2013 via Hootsuite
McKenna: System: not much to talk about. Built to handle PII capacity. 5 Gbps links robust, able to get to 14 Gbps #ionworld2013
2:26pm October 22nd 2013 via Hootsuite
McKenna: All core data rates have been tested, timeline listed for CMOS design, mfg. 30 layers of masks. Today: in mfg #ionworld2013
2:24pm October 22nd 2013 via Hootsuite
McKenna: Data transfer: Cisco routers 100 Gbps; PII will go 120 Gbps. 120 1Gb ethernet cables. 36K Netflix movies at once! #ionworld2013
2:23pm October 22nd 2013 via Hootsuite
McKenna: Sensor illustrated, then sensor plate, to the transistor. P1 electronics with P2 wells on top: 1/4 wells usable #ionworld2013
2:19pm October 22nd 2013 via Hootsuite
McKenna: Used a to-scale HiSeq cluster and compared it to the size of a PII well. Well geometry, sensor structure, materials #ionworld2013
2:18pm October 22nd 2013 via Hootsuite
