#AACR19 Adey sci-ATAC-seq; sci-ATAC-seq in TNBC, PDAC; beyond it and porting to Celsee. ATAC-seq: focus on chromatin-accessible areas
3:33pm April 2nd 2019 via Twitter Web Client
#AACR19 Andrew Adey (OSHU) Single-cell omics with microwell miniaturization. Most sc studies are scRNA-seq. But - r… https://t.co/GsT3xeu3ft
3:32pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson Concl: They want to offer test bundles/offerings to differentiate. Leverage high param CTC analys… https://t.co/zNLYtgf1W1
3:31pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson Showing recovery of InCellDx BioINK kit results - maximizing CTC recovery. Sample prep - they hav… https://t.co/uKjAxD9Z8w
3:30pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson High PD-L1 exp and anueploidy correlated with tumor load. CTC prediction from primary tumor. Show… https://t.co/WUFxx165wF
3:28pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson Found they can go to 1:1,000,000 cells 'highly linear'. Found high var in tumors.
3:25pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson Gemini study: paired primary tumor/blood comparison. 30+ marker capabilities - Beckman Cytoflex Rx.
#AACR19 Patterson In 25 lung ca - PD-L1 increases as tumor cell clones increase. In prostate: have a 'ton of circ e… https://t.co/LxIPaTP5X5
3:24pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson Simultaneous detection of protein, mRNA, and DNA. Shows PD-L1 expression as a function of DNA con… https://t.co/VWiQFbQmOh
3:21pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson Epithelial cells (CK+) in urine: 6% InCellMax vs 30% Preservemyt rejection rate. Shows their menu… https://t.co/IyuH56zAF7
3:20pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson What drives this: InCellMax preservative; non-crosslinking; single-step in-situ hyb/FISH w/o prot… https://t.co/d0M83QurBz
3:18pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson InCellDx can use blood, urine. As they are Dx, InCellPrep is a class I to make a single-cell susp… https://t.co/G18GXDlHwi
3:17pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson Tumors are highly heterogeneous; yet we put them in a blender. Originally: used Southerns to look… https://t.co/zN2NWNrTD3
3:16pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson Think about the translation into the clinical realm. Personalizing of medicine: goes much deeper,… https://t.co/MABaUPimuD
3:15pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson Measuring HIV in plasma: viral load has 1000:1 ratio of non-infectious to infectious, 'the ultima… https://t.co/v8QiYgpEU9
#AACR19 Patterson InCellDx offers comprehensive, quantitative, simultaneous, and multiplexed. 'The cell is everything'.
3:14pm April 2nd 2019 via Twitter Web Client
#AACR19 Patterson (InCellDx) Single cell-by-cell diagnostics. Have been able to translate all reagents used via flo… https://t.co/Xj5MZmExh9
3:13pm April 2nd 2019 via Twitter Web Client
Celsee #AACR19 Ranade sc Cytometry, sc proteo-genomics, sc transcriptomics, also open platform.
3:12pm April 2nd 2019 via Twitter Web Client
Celsee #AACR19 Ranade 250K microwells, 25K cells loaded. Capture eff: irrespective of scale (2.5K cells to 22.5K cells)
3:11pm April 2nd 2019 via Twitter Web Client
Celsee #AACR19 Ranade Bead loading is simple via gravity; cap put on, excess beads washed. Cells lysed, mRNA hyb to… https://t.co/XWW0GfcAiv
3:10pm April 2nd 2019 via Twitter Web Client
Celsee #AACR19 Ranade Dissociate tissue, optional Ab tagging of surface proteins. pipette onto slide, optional QC via microscopy.
3:09pm April 2nd 2019 via Twitter Web Client
Celsee #AACR19 Ranade Celsingle is open microwell slide; hexagonal features; recover >70% of input cells.
3:08pm April 2nd 2019 via Twitter Web Client
Celsee #AACR19 Ranade Also open platform - two different types of slides: Celsingle slide, Preparation slide. (Firs… https://t.co/1Ogl81GRwS
3:07pm April 2nd 2019 via Twitter Web Client
Celsee #AACR19 Ranade Building tools on sc technologies: addresses deficits - gravity is gentle, visual cell-loadin… https://t.co/rCcHobAJi9
Celsee #AACR19 Ranade: Is developing a system called Genesis; starting with the 'why': sc approaches gives understanding of heterogeneity
3:06pm April 2nd 2019 via Twitter Web Client
#AACR19 Celsee Workshop: Swati Ranade (Dir R&D), Bruce Patterson (CEO Incell Diagnostics) Andrew Adey (OSHU)
3:04pm April 2nd 2019 via Twitter Web Client
Thompson #AACR19 Concl - a powerful new tools to map the immune landscape of breast tumors; stromal compartments ma… https://t.co/g7jmWWtCtb
10:40am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 Shows heatmap of FN1, CD86, IDO1 - myeloid and ECM markers in CD68-enriched assoc'd with recurr-free survival
10:37am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 CD68enriched Myeloid/macrophage enriched stroma associated with positive outcome. One gene robustl… https://t.co/A8bjn933sk
10:36am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 Clear differences between tumor and stroma; shows PCA plot, looks at GEX of PD-L2 in CD68 enriched… https://t.co/E6fjblP4JG
10:34am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 300 um ROI chosen by hand; mask for PanCK+; draw for SYTO12+/PanCK-. Shows S:N numbers (protein) a… https://t.co/oXj5r4DwpP
10:30am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 12 ROI's, 42 target proteins, 5 segments/sample. PanCK, CD45, CD68, SYTO13. (tumor, leukocytes, ma… https://t.co/Z7N3xk3lIo
10:28am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 BC360/IO360 has generated a lot of observations; giving the imptc of looking at TME. Shows chart o… https://t.co/4SOPys6aXP
10:26am April 2nd 2019 via Twitter Web Client
Thompson #AACR19 FinXX trial - 120 TNBC, two arms T + CEF (5-fluoruacil) / Tx + TEX (capecitabine). Profile immune… https://t.co/bfB6BsLCRc
10:25am April 2nd 2019 via Twitter Web Client
Aubrey Thompson (Mayo Clinic FL) #AACR19 Had the instrument for about 6w; not an immunol but working on gene expression.
10:23am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 AbCam a partner, will be making antibodies compatible with GeoMx; ACD another with FFPE-compat sing… https://t.co/INdG9HD7QQ
10:20am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 Digital, multi-analytie, single-molecule counting, 10^6 dynamic range, single-cell limit of detecti… https://t.co/KdzAgBUaZz
10:18am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 Two different invasive boundaries compared: at the GEX level, completely different.
10:16am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 Coming soon: unlimited multiplexing; showing 1500 genes Q4, in lab above 10,000 genes. Shows 1412 g… https://t.co/Se40PIt95A
10:15am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 Shows different types of profiling - along a boundary, with a gradient. Two of four references:… https://t.co/4dAxVmGROu
10:14am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 PanCK lights up tumor-spec cells; mirrors will collect only those cells. Image can be reversed - only TME; 30s apiece.
10:09am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 All antibodies applied; then four colors illuminate ROI. Tags collected w/ x-y coordinates. Deliver… https://t.co/MFvEvgJYty
10:07am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 The microscope not only the reader, but also capable to cleave the tags and collect with x-y coordinate info.
10:05am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 Instead of colors, use short oligo tags to encode the ID of Ab or mRNA sequence. And the barcode ta… https://t.co/gZigXgmYBl
10:04am April 2nd 2019 via Twitter Web Client
Beechem #AACR19 Continuous discovery cycle: bulk gene exp to spatial profiling. 5y ago the concept: measure 10K things in a microscope.
Beechem #AACR19 The idea: same basic idea, using digital optical profiling, and using the technology and moved into spatial domain.
10:02am April 2nd 2019 via Twitter Web Client
Joe Beechem (Nanostring) @nanostringtech Multi-analyte profiling using the GeoMx digital spatial profiling. #AACR19
Heath #AACR19 B-cell antigens have discontinuous epitopes. The challenge - most important yet most complicated to get to.
7:46am April 2nd 2019 via Twitter Web Client
Heath #AACR19 CD Tcell antigens are becomign increasingly well understood - like a hotdog in a bun. CD4 T cell anti… https://t.co/FuVbrkwW9w
7:45am April 2nd 2019 via Twitter Web Client
Heath #AACR19 Shows pMHC library construction and protein production - cost-effective, very specific.
7:43am April 2nd 2019 via Twitter Web Client
