#AACR19 Akoya Rimm: At SITC: Steve Lu (Hopkins) to compare biomarker assay modalities (PD-L1) and make AUC curves.… https://t.co/kLamwfJ1Wc
2:03pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: His disclosure slide is busy because biotech is moving this from the lab to the clinic. Will ta… https://t.co/xQl864FR6j
2:02pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya David Rimm (Yale) Dir of Yale Pathology Tissue Services title: Immunofluorescence - from the lab to the clinic?
2:01pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Working on signal amplification (lowering damage to lower exposure times). Typically… https://t.co/xqkljH1Xta
1:59pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Shows cell proximity by cell-type from the 2D analysis of breast cancer data.
1:57pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: FF lymph node from breast cancer pt: 29 marker staining; DAPI stain with .fcs file (… https://t.co/a10Bg2Nh4T
1:55pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Shows overlay data of FFPE human renal cell carcinoma with CODEX tissue analysis, al… https://t.co/o4qcJ95VVK
1:53pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Shows signal saturation / noise with titer data for spec Abs. Look at +/- countersta… https://t.co/MEDIJ8XdRH
1:50pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Also comparing tissues to databases with known targets. Threshold image of signal/no… https://t.co/wNWNyOYu9S
1:49pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Microscopes are controlled directly by their software. Image processing SW and image… https://t.co/7lVmhA4eJ3
1:48pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Tissue slices onto coverslips, inserted onto stage + gaskets; fluids controlled by Akoya instrument.
1:47pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Dist of Abs 'across 4 channels'. Four fluors, can use Alexa 750. CODEX integrates wi… https://t.co/KWkdzdYkvu
1:46pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Shows no signal dropoff from cycle 1 to cycle 11. Reagents: kits for staining, conju… https://t.co/KEiI5x87c3
1:45pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Shows CD38 staining of signal ROI (threshold) cp to noise ROI. Shows high signal/noise, with low carryover.
1:43pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Repeating process 16+ times. Shows movie - human tonsil, shows same tissue stained 1… https://t.co/0o4siUXSWd
1:41pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Shows instrument - CO-Detection by inDEXing - reagents, fluidics control, software.… https://t.co/oRBitRaOpg
#AACR19 Akoya Kennedy-Darling: Busy slide with TME - DC, NK, B, Th, Tk, adipocytes. Overlaying checkpoints - 39 markers.
1:40pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: highly multiplexed platform CODEX - starts with description of TME: contributions fr… https://t.co/HSVrKAG4xc
1:39pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Lo: Unmixed, annotated ROI's up to 9-colors using tunable filter tech. CODEX: Julia Kennedy-Darling Dir R&D
1:38pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya CEO David Lo: Phenoptics 2.0 is a Perkin Elmer-dev system, 7 color Opal for Polaris, 6 min (10x-20x faster) whole slide scans.
1:37pm March 31st 2019 via Twitter Web Client
RT @CNET: Tonight at 9pm #TheInventor premieres on HBO, and it explores the whole bloody mess behind Theranos, Elizabeth Holmes. https://t…
2:13pm March 18th 2019 via Twitter Web Client
My answer to: Is whole genome sequencing, with companies like Nebula Genomics, the same type of genetic testing per… https://t.co/DHmcJT1tv9
9:38am March 16th 2019 via Twitter Web Client
RT @mason_lab: "The entirety of the internet’s data can fit on a DNA storage device that is the size of a shoebox, but it is $1,000/MB toda…
1:09pm March 13th 2019 via Twitter Web Client
RT @BioMickWatson: We have a fantastic programme https://t.co/y7wkyR9pSt #GenomeScience2019 #Edinburgh https://t.co/cGnaLLh0J2
8:25am March 11th 2019 via Twitter Web Client
RT @coletrapnell: Here's a little movie zooming in Monocle 3's trajectory for the myocytes, which emerge from the mouse embryo's "mesenchym…
8:05am March 9th 2019 via Twitter Web Client
RT @ribozyme: More cool graphs at #AGBT19 - contacts at homologous chromosomes by Wu https://t.co/n5LwWh9ehG
8:02am March 9th 2019 via Twitter Web Client
RT @KevinADavies: “Think about a 14 meter-long (50 ft) extension cord or rope going through your hand every second. That this works at this…
7:17am March 6th 2019 via Twitter Web Client
@Biud This cool 'subway map' of #AGBT19 sponsors is great. I did notice though that one sponsor not on here is… https://t.co/RLggquByUR
7:22pm March 4th 2019 via Twitter Web Client in reply to Biud
@pathogenomenick I think my problem at #AGBT19 was not being able to spot your unique visage in a crowd. It was a p… https://t.co/emKUwtiJLZ
3:59pm March 4th 2019 via Twitter Web Client in reply to pathogenomenick
RT @pathogenomenick: @DaleYuzuki Thanks Dale! Our first ‘proper’ video should be up tomorrow. Btw I did see you at Marco but you didn’t spo…
3:54pm March 4th 2019 via Twitter Web Client
RT @longreadclub: Thanks for the write up. At least we had a great location to film in ;-) https://t.co/LQ4TVKflQn
3:53pm March 4th 2019 via Twitter Web Client
RT @longreadclub: It’s here - the first complete chromosome assembly for the X chromosome. Enabled by long reads. #AGBT19 #longreadclub htt…
8:35am March 4th 2019 via Twitter Web Client
Farewell #AGBT19 and thank you to all the kind attendees that have introduced themselves! See you next year at… https://t.co/Q1Tj4k372Q
4:14pm March 2nd 2019 via Twitter Web Client
Q: RNA-seq? Tan #AGBT19 They are testing but insufficient time to generate data here.
4:07pm March 2nd 2019 via Twitter Web Client
Q: Antibody's epitope? Tan #AGBT19 Two parts - both the label and base, helping specificity.
4:06pm March 2nd 2019 via Twitter Web Client
Tan #AGBT19 Sequencing everyone on earth. 1000 sequencing labs, 1000 genomes/day. Does some math, sequence 7B population in 50y.
4:05pm March 2nd 2019 via Twitter Web Client
Tan #AGBT19 Have built about 7 instruments and are undergoing testing now.
4:04pm March 2nd 2019 via Twitter Web Client
Tan #AGBT19 Listed at $1M/instrument. $5/Gb. 60-samples / day at 30X WGS, up to 20K samples/year.
Tan #AGBT19 5B reads/flow cell. x4 = 20B reads/run.
4:01pm March 2nd 2019 via Twitter Web Client
Tan #AGBT19 PCR-free, combining no PCR accurate and low-cost, along with stLFR on same instrument providing phasing… https://t.co/oBHmHC96Rs
Tan #AGBT19 Do not see signal 'suppression' (might mean 'compression') Potential to go to 800bp SE and 600bp PE. Ca… https://t.co/nuV5kxJ1sy
4:00pm March 2nd 2019 via Twitter Web Client
Tan #AGBT19 WGS PCR-free - duplicate rate <2%, index hopping 0.0001%. Today: intorduce coolNGS in cPAS. Unlabeled nucleotides, native bas
3:57pm March 2nd 2019 via Twitter Web Client
Tan #AGBT19 DNBSeq is rolling circle ampl; 'zero PCR accumulative errors'. Patterned array allows only one DNB boun… https://t.co/zZA0m0BfQe
3:56pm March 2nd 2019 via Twitter Web Client
Tan #AGBT19 T7: 6 Tb/PE150 run; <24h/run.
3:54pm March 2nd 2019 via Twitter Web Client
Tan #AGBT19 Accuracy, speed, throughput, stability, cost: going to 5G communications, what we have 3G and 4G is slo… https://t.co/y3YvgckGSe
3:53pm March 2nd 2019 via Twitter Web Client
Roy Tan (MGI Americas) #AGBT19 MGISEQ-T7 sequencer powered by DNBseqTM technology opens a new era of “broadband” sequencing
3:52pm March 2nd 2019 via Twitter Web Client
Macosko #AGBT19 Concl: 10um resolution, genome-wide gene exp, developmental trajectories, further tech dev includes… https://t.co/vxTTll9JUV
3:49pm March 2nd 2019 via Twitter Web Client
Macosko #AGBT19 Glial scarring post cortical injury - can find specific cell types after 2w timepoint, describes sp… https://t.co/tEDainHQP4
3:48pm March 2nd 2019 via Twitter Web Client
Macosko #AGBT19 Used NMF regression to use scRNA-seq to come up with a cell-type 'dictionary', and super-impose on projections.
3:44pm March 2nd 2019 via Twitter Web Client
Macosko #AGBT19 Have done expts in liver, kidney. Mapping gene expression patterns, known sc dataset and determine where they map.
3:42pm March 2nd 2019 via Twitter Web Client
