Roche: Showed MiSeq comparative method, good concordance; and full-process experiments. Can use 'Semi Quantative In… https://t.co/ugU9jW7EBf
12:14pm November 15th 2017 via Hootsuite
Roche: For BRAF/NRAS in plasma: spiking expt. 7 BRAF / 9 NRAS down to 0.03%; others at 0.13%,. 5 at higher MAF #AMP2017
12:12pm November 15th 2017 via Hootsuite
Roche: 1 at 5% AF, 4 at 2.5% MAF, 31 at 1% MAF from plasma. Using FFPE showed 100% at 5%, all but two at 2.5% MAF done in duplicate #AMP2017
12:11pm November 15th 2017 via Hootsuite
Roche: KRAS v2: 28 mutations in exons 2/3/4; also includes codon 61, 145, 49, 117. Total of BRAF/NRAS and KRAS is 6… https://t.co/finX4T1DpO
12:08pm November 15th 2017 via Hootsuite
Roche: 11 BRAF, 25 NRAS; several exon 11 mutations, also K601E for exploratory purposes. For NRAS, Exon 4 and 3 as well. #AMP2017
12:06pm November 15th 2017 via Hootsuite
Roche: Expanding the Scientific Body of Knowledge with the New BRAF/NRAS Mutation Test and KRAS MutationTest v2 for… https://t.co/3g8iUvwqPS
12:05pm November 15th 2017 via Hootsuite
Q: Specific recommendations for pre-analytical handling? Promega: Have used both EDTA and Streck, recommend double spins. #AMP2017
11:37am November 15th 2017 via Hootsuite
Promega: Developed a concentration cartridge for 1mL binding events to process up to 4mL plasma. 1mL spread across… https://t.co/bn1B4mo8FW
11:27am November 15th 2017 via Hootsuite
Promega: Have Maxwell RSC DNA FFPE and RNA FFPE kits; xylene-free protocol; manual or automated; <1h extraction time #AMP2017
11:18am November 15th 2017 via Hootsuite
Promega: Offers portal software to track samples across modules, network accessible, unified reporting. #AMP2017
11:15am November 15th 2017 via Hootsuite
Promega: Now has Maxprep liquid handler for preprocessing: ProK, lysis; incubate, plunger placement and elution. Tr… https://t.co/dcHqaSbRJh
11:11am November 15th 2017 via Hootsuite
Promega: "Modular Automation Workflows for Maxwell® Nucleic Acid Extraction of Molecular Oncology Sample Types Such… https://t.co/ybgwLufL1m
11:10am November 15th 2017 via Hootsuite
Promega: Maxwell RSC 48 now equipped with a camera. Checking what's on the table vs. what you've said is there. 2x… https://t.co/jCkGIMVUOJ
11:08am November 15th 2017 via Hootsuite
RT @Tecan_Talk: Visiting #SLC for #AMP2017? Don't forget to take a selfie with the ‘You Are Here’ art installation… https://t.co/e42SUmp7iP
10:55am November 15th 2017 via Hootsuite
Q: Is the Oncomine Dx now available? Duncan: Yes, since Sept. Q: Concern about false positives? Ma: Strict cut-off:… https://t.co/TIbc9EUwR0
10:49am November 15th 2017 via Hootsuite
Ma: "This is not the future, this is now." Further increase in sens. would be through error-correction algorithms;… https://t.co/q2irU49tc1
10:40am November 15th 2017 via Hootsuite
Ma: Found a T790M EGFR resistance mutation, from two separate aliquots. Same mutation showed up from separate sampl… https://t.co/RR2nBx4XDT
10:38am November 15th 2017 via Hootsuite
Ma: Have found all mutations found in tumor tissue biopsy (EGFR, KRAS, PIK3CA etc) Goes through one case: Stg IV, P… https://t.co/WFlVjc5qom
10:37am November 15th 2017 via Hootsuite
Ma: 'In our case, a false-positive is definitely a no-no.' Shows a chart of genes, alleles, and allele frequencies… https://t.co/15R5V9QK82
10:36am November 15th 2017 via Hootsuite
Ma: Sens 85%, Spec 99%, down to 0.1%. Sens achieved via barcoding. Allows distinguishing error from true mutation #AMP2017
10:33am November 15th 2017 via Hootsuite
Ma: Challenges: Pt frailty - 17% of biopsies resulted in complications; insufficient material (5% of EGFR single-ge… https://t.co/GBFVDXqw86
10:32am November 15th 2017 via Hootsuite
Ma:Stats around lung ca: 80-85% NSCLC, 15-20% SCLC. EGFR, BRAF, ALK/ROS1 fusion, MET amp all offer specific treatment options #AMP2017
10:31am November 15th 2017 via Hootsuite
Ma: A needle in a haystack, 'but the needle looks different'. Shows sensitivity chart - Sanger at 10-20%, Pyro 5-10… https://t.co/7TWGoBAnTv
10:30am November 15th 2017 via Hootsuite
Ma: cfDNA released from both healthy and tumor cells; apoptosis, necrosis and secretion. Unstable: 20-30ng per 5mL,… https://t.co/iCpAvbWexT
10:29am November 15th 2017 via Hootsuite
Ma: Tumors shed intact cells (CTCs), cellular components (cfDNA and cfRNA) and also exosomes, cell-membrane derived vesicles #AMP2017
10:27am November 15th 2017 via Hootsuite
Ma: Also biospy tissue limited: greater demand due to molecular profiling, surgery is costly (about $37K cost) #AMP2017
Ma:Points out that biopsy is invasive - patient may be in poor condition, or tumor inaccessible; impractical for pr… https://t.co/YefYHB7Ks9
10:25am November 15th 2017 via Hootsuite
Ma: Defines liquid biopsy, with figure from Diaz et al ASCO 32:579 (2014). Advantages: real-time eval and monitorin… https://t.co/9iuiwwiWrQ
Charles Ma (Cancer Genetics Inc) Thermo workshop: "Cell-free DNA and lung cancer clinical research" #AMP2017
10:23am November 15th 2017 via Hootsuite
Thermo: conlcude 'NGS can simplify elaborate testing algorithms and consolidate workflow.' Speaker: Daniel Duncan,… https://t.co/P2SaoitCl3
Thermo: Graphed number of slides consumed per single-gene test (as many as 18 slides for 7 genes) vs 1 for Oncomine… https://t.co/lDEJFJNvSP
10:21am November 15th 2017 via Hootsuite
Thermo: Retrospective analysis at IASLC world conf in lung ca: shows distribution that Core Needle Biopsy (n=1,029)… https://t.co/ZJfCJVyTuG
10:18am November 15th 2017 via Hootsuite
Thermo: Shows NCCN guidelines - BRAF V600E, ROS1, EGFR exon 19 del and Exon 21 L858R. Add'l targets listed, points… https://t.co/59uBLZKYfx
10:16am November 15th 2017 via Hootsuite
Thermo Oncomine Dx Target test: specific sample prep, cDNA synthesis, target test, OneTouch 2 Dx, PGM Dx and Torren… https://t.co/orPvu8Q8Tb
10:15am November 15th 2017 via Hootsuite
Thermo Fisher workshop Speaker: From Cancer Genetics @cancer_genetics: Reviews typical stepwise approach: 7-8d for… https://t.co/h2PFKDcDkU
10:13am November 15th 2017 via Hootsuite
RT @matthewherper: Don't miss this @amy_harmon piece! https://t.co/pzwpzLWY7h
7:40am November 15th 2017 via Hootsuite
RT @NatureComms: Two major pathways for cancer and aging, telomeres and PI-3 kinase, shown to be linked. https://t.co/e70czVRFdE
6:31am November 15th 2017 via Hootsuite
New post: Preview of Association for Molecular Pathology 2017 #AMP2017 https://t.co/lMtWfmbcZ6
5:44pm November 14th 2017 via WP to TWTR on Yuzuki.org
RT @JMDiagn: #AMP2017 meeting Abstracts, FREE in JMD: https://t.co/nkWO9gLbnq. Light reading for traveling to this years @AMPath meeting!…
1:49pm November 14th 2017 via Hootsuite
Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy | Nature Communic… https://t.co/GOravZIe5B
1:47pm November 14th 2017 via Hootsuite
RT @AMPath: Your bags are (mostly) packed, your posters/slides are prepped, and you're almost ready for #AMP2017. There's one important thi…
8:06am November 14th 2017 via Hootsuite
RT @Pillar_Bio: Did you hear about @Pillar_Bio and @illumina collaborating on NGS-based in-vitro diagnostics? https://t.co/Yzkh743Bau Look…
4:44pm November 13th 2017 via Hootsuite
RT @Pillar_Bio: Interested in a new clinical oncology assay technology? Visit the @Pillar_Bio booth 1724 at #AMP2017
6:37am November 13th 2017 via Hootsuite
RT @ontariogenomics: Why precision research may lead to blockbuster, not customized, medicines #PrecisionMedicine https://t.co/NVml3dmDrn
1:45pm November 7th 2017 via Hootsuite
RT @psufka: I agree with @EricTopol: Twitter is the *best* way to keep up with science & medicine. #ACR17 https://t.co/poyf16FyvK
12:34pm November 7th 2017 via Hootsuite
NIH awards to test ways to store, access, share, and compute on biomedical data in the cloud | NIH https://t.co/1iS4B2hQbu
9:20am November 7th 2017 via Hootsuite
Omics! Omics!: A Nucleotide Mixture-Based Error Correcting Short Read Chemistry https://t.co/7sYT76j50N
8:35am November 7th 2017 via Hootsuite
Carbon nanofibres linked to mesothelioma | Cosmos https://t.co/fffQzIwwnA
7:35am November 7th 2017 via Hootsuite
Deadly lung cancers are driven by multiple genetic changes https://t.co/2lJptzpr6u
6:30am November 7th 2017 via Hootsuite
What Happens When You Put 500,000 People's DNA Online - The Atlantic https://t.co/Xs4NInrpO0
5:30am November 7th 2017 via Hootsuite
