Beechem #AGBT19 Technical details on DSP is poster 514 Friday 440pm; mapping pathways poster 614 also Friday 440pm.… https://t.co/2RmLPGpgwn
4:44pm February 28th 2019 via Twitter Web Client
Beechem #AGBT19 Molecular profilers and imagers 'live in different worlds' Volcano plots, tSNEs, etc are where the… https://t.co/hq9EkJujDY
4:42pm February 28th 2019 via Twitter Web Client
Beechem #AGBT19 Poster 514 image: barcoded probes along length of mRNA, and shows 10 data points per xcript. Dropou… https://t.co/y9oc4o0nzR
4:40pm February 28th 2019 via Twitter Web Client
Beechem #AGBT19 Shows data from Poster 614, the chemokine and cytokine regions are different. Active invasive margin cp to inactive one.
4:38pm February 28th 2019 via Twitter Web Client
Beechem #AGBT19 Genentech actually wrote up a review '18 https://t.co/TMzHl7ocld before the instrument was launched.
4:36pm February 28th 2019 via Twitter Web Client
Beechem #AGBT19 How the UV light is focused: digital micromirrors. Shows CRC; visible wavelength PanCK antibody, gr… https://t.co/OAKkmI8NyF
4:34pm February 28th 2019 via Twitter Web Client
Beechem #AGBT19 Microcapillary drinks the probes off the tissue in X-Y coordinate space and deposits into a 96-well… https://t.co/VMEIvjMsbD
4:31pm February 28th 2019 via Twitter Web Client
Beechem #AGBT19 Affinity probes can be FISH for RNA with oligos instead of fluors. Or oligo-labeled antibodies. The… https://t.co/BJyLlESZZu
4:30pm February 28th 2019 via Twitter Web Client
Beechem #AGBT19 4y ago in Gordon Mill's office at MD Anderson - want 100K things off a tissue slice. Instead of ass… https://t.co/LRRqUKxwh7
4:28pm February 28th 2019 via Twitter Web Client
Beechem #AGBT19 Will update on Hyb & Seq launch 2021. GeoMx DSP focuses on very difficult FFPE samples. Discovery -… https://t.co/NEyMQt
4:27pm February 28th 2019 via Twitter Web Client
Beechem #AGBT19 3D biology - mRNA, proteins, miRNAs, talked about here at AGBT for a few years. Now: as a microscop… https://t.co/7D6y6Xa5TF
4:26pm February 28th 2019 via Twitter Web Client
Joe Beechem (Nanostring) #AGBT19 Emphasis is not on technology of the GeoMx Digital Spatial Profiler, but rather on… https://t.co/pYnj2kaLCH
4:25pm February 28th 2019 via Twitter Web Client
Neafsey #AGBT19 Issue driven by the price of gold (!) and 'everyone is complicit'. Shows a gold toilet, and use as… https://t.co/9LcMSFa4OS
4:03pm February 28th 2019 via Hootsuite Inc.
Neafsey #AGBT19 Talks about Arteminsin resistance, worldwide distribution, evidence it is on the margin, and that r… https://t.co/MyprOYmYXW
4:00pm February 28th 2019 via Hootsuite Inc.
Daniel Neafsey (Harvard) #AGBT19 Rewinding the tape: Historical pathogen population genomics using selective genome… https://t.co/BmeJuZCIDk
3:56pm February 28th 2019 via Hootsuite Inc.
10X Genomics #AGBT19 Introduces Chromium Connect, cells to sequencing library automation in their suite. "I need my… https://t.co/7ulAWsOaLh
12:59pm February 28th 2019 via Twitter Web Client
10X Genomics #AGBT19 Visium Spatial platform available 'H2 2019'.
12:58pm February 28th 2019 via Twitter Web Client
10X Genomics #AGBT19 Improved resolution: 100um spots, 200um center-to-center. Next: denser features and resolution… https://t.co/GTxQW10LTk
12:57pm February 28th 2019 via Twitter Web Client
10X Genomics #AGBT19 Have already shortened (in 3 mos) from 3days down to 9h. Have increased sensitivity >2x; 3x tr… https://t.co/tARBPMc
12:55pm February 28th 2019 via Twitter Web Client
10X Genomics #AGBT19 Spatial is 'coming soon' - end-to-end. Protocols for every step, slide and reagent kits, softw… https://t.co/HghRWvmgNw
12:54pm February 28th 2019 via Twitter Web Client
10X Genomics #ABRF19 Human heart as part of human cell atlas; week 6.5 and 15 cell types, shows t-SNE plot, and det… https://t.co/YjxMgHnw6w
12:51pm February 28th 2019 via Twitter Web Client
10X Genomics #ABRF19 Paper also noted discordances. Biorxiv preprint - looking across multiple slices, and across tumors.
12:49pm February 28th 2019 via Twitter Web Client
10X Genomics #AGBT19 Can start with an image, or start with clustering of genes. In '18 Nature Comm… https://t.co/66424C3Jtd
12:46pm February 28th 2019 via Twitter Web Client
10X Genomics #AGBT19 Shows Pearson 0.97 biological replicates on different arrays. Power - two complementary dataty… https://t.co/PgYGhm5KJL
12:44pm February 28th 2019 via Twitter Web Client
10X Genomics #AGBT19 Announces spatial transcriptomics. reviews '16 Science https://t.co/wVxn4MeNR4 Demonstrated on… https://t.co/RRmlLkK8Gw
12:43pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 VDJ database online https://t.co/TQYAd6x8Ds 2,336 TCR-Ag spec 10 Ags from first Nature paper; 2… https://t.co/PxkJU11wjI
12:39pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Dataset: 80M input CD8+ T-cells (from four donors). 44 pMHC multimers, 6 neg controls. Data from 200K single cells.
12:36pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Points to a pair of papers in 17 Nature https://t.co/uwTng9b5Z3 https://t.co/G8yySzlx7g pointi… https://t.co/0kasWvTjXF
12:35pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Direct VDJ sequences between two cells. TCRa/TCRb, bind to peptide Ag by MHC I or MHC II alpha/… https://t.co/YTBaHdvEhH
12:34pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Fig from '18 Nature Rev Immunol https://t.co/aVb1j3klk8 Partner with Immudex for reagents to ma… https://t.co/7rr8zaTaiv
12:32pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Simultaneous RNA and protein detection in 15,733 cells, from new PDF (not on website yet but in… https://t.co/FxJcqciIBR
12:30pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Feature barcoding: Abs conjugated to barcodes. Partnered with BioLegend for Ab's.
12:29pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Coming soon - compatible products from Millipore Sigma. Second: quantifying cell surface proteins.
12:27pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Points to recent '19 Cell https://t.co/7v0yHgjQsp from Shendure's group.
12:26pm February 28th 2019 via Twitter Web Client
Stubbington #AGBT19 Barcoding 'things' - CRISPR perturb-seq. Introduce pool of gRNAs into a pool of cells, examine… https://t.co/l7l6NFlfAs
12:25pm February 28th 2019 via Twitter Web Client
Mike Stubbington (10X Genomics) #AGBT19 Workshop Increased sens with sc gene expression v3. A year ago: announced c… https://t.co/9dQsh7WEyJ
12:23pm February 28th 2019 via Twitter Web Client
10X Workshop #AGBT19 what's next? sc CNV / epigenomics / spatial gene expression / surface protein expression
12:22pm February 28th 2019 via Twitter Web Client
10X workshop at #AGBT19 Last example - mechanisms of I-O resistance from https://t.co/JUOH310xK4 Mechanism persists… https://t.co/DZtww23dYx
12:21pm February 28th 2019 via Twitter Web Client
Lau #AGBT19 Click chemistry conjugations of analytes onto solid phase; target enrichment and elution. Uses modified… https://t.co/hti9TqWTlT
11:53am February 28th 2019 via Twitter Web Client
Billy Lau (Stanford) #AGBT19 A DNA 'time machine' for repeated interrogation of cell-free DNA and FFPE. Approach: s… https://t.co/9qfRylDLha
11:52am February 28th 2019 via Twitter Web Client
Ettwiller #AGBT19 CAGE detects only TSS from Pol II transcripts. Other Pols (I, II, III and mitochondrial pol) prod… https://t.co/onDOjI0JYi
11:51am February 28th 2019 via Twitter Web Client
Lee Ettwilller (NEB) #AGBT19 Poster 503 ReCappable Seq for TSS interrogation. TSS better define transcripts, and ac… https://t.co/f7385FOFbs
11:49am February 28th 2019 via Twitter Web Client
Shapiro #AGBT19 Also points to this '17 Nature Methods using a method called histoCAT https://t.co/LlCew8cKkW
11:43am February 28th 2019 via Twitter Web Client
Shapiro #AGBT19 Induced in epith compartment. '18 Cell CODEX https://t.co/JaQYEljIql and points to BioRxiv of GeoMx https://t.co/GW80Eo2U51
11:41am February 28th 2019 via Twitter Web Client
Dennis Shapiro (Broad) #AGBT19 Poster 119 Reconstruction of the colorectal cancer landscape. Induced APC deletion,… https://t.co/15A0tui9kE
11:40am February 28th 2019 via Twitter Web Client
Chen #AGBT19 Poster 103, extracting longitudinal data from a single lung patient. Drill-down into timeline, automat… https://t.co/xeiOLkKIF0
11:38am February 28th 2019 via Twitter Web Client
Chen #AGBt19 phot of the Centrellis Sema4 platform. Clinical test, Pt mgmt, Rx. https://t.co/3hCQ0I0Lil
11:37am February 28th 2019 via Hootsuite Inc.
Flash talk 1 #AGBT19 Rong Chen (Sema4) Advancing precision medicine through data science. Centrellis Health Intelli… https://t.co/FwIjfGVbS8
11:36am February 28th 2019 via Twitter Web Client
Q: Library prep? PE? Clark #AGBT19 Standard approaches; SE to begin with, PE sometime in the future.
11:32am February 28th 2019 via Twitter Web Client
Clark #AGBT19 Small, inexpensive, scalable, real-data, range of sample types. Can 'sense nucleic acids, proteins and cells'. H2 '19 launch.
11:31am February 28th 2019 via Twitter Web Client
