Chen #AGBT19 Onto case studies of how this type of data could be used: construct tissue architecture. ID regional s… https://t.co/Lc0jjjGHr9
1:12pm February 27th 2019 via Hootsuite Inc.
Chen #AGBT19 (NB - said bioRxiv paper would go up today with this technique, not there yet.) Shows validation, diff… https://t.co/J6Z4VodZsP
1:10pm February 27th 2019 via Hootsuite Inc.
Chen #AGBT19 All up-front: put slice of tissue, map gene expression in space.
1:09pm February 27th 2019 via Hootsuite Inc.
Chen #AGBT19 Describes DropSeq: take beads (10um), bead w/PCR handle, spatial barcode, UMI. Put beads on surface; u… https://t.co/YYYr8EJgxb
1:07pm February 27th 2019 via Hootsuite Inc.
Chen #AGBT19 Describing the problem over coffee w/inventor of Drop-seq. Methods are in-situ (Starmap, Merfish, ExFi… https://t.co/QJHwozpyoQ
1:05pm February 27th 2019 via Hootsuite Inc.
Chen #AGBT19 Shows a diagram from this '15 Cell (Drop-Seq) https://t.co/QHv1Bv7RC7 Slide-seq - genome-wide expression at 10um
1:03pm February 27th 2019 via Hootsuite Inc.
Fei Chen (Broad Institute) #AGBT19 Tools for in-situ molecular interrogation spanning the resolution and throughput spectrum
1:01pm February 27th 2019 via Hootsuite Inc.
Q: H&E stained? Beechem #AGBT19 Can use it as an overlay to help visualize area to focus in on.
12:57pm February 27th 2019 via Hootsuite Inc.
Q: How large an area? Beechem #AGBT19 1-2 cm on a side. Typically 10-20 ROI's, 10 mm2 each. Slides can be reprobed on different days.
Beechem #AGBT19 Summarizing, 20K barcodes, 'add another zero for next year'; up to 20 sections / day throughput.
12:55pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Thursday @nanostringtech workshop Thursday afternoon (John McPherson), poster 514 Friday, plenary b… https://t.co/VlzELYIUsL
12:54pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 a plot of 5K mRNA vs 418 probes - scaling. https://t.co/58HfGFcgGi
12:50pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Shows exon-level PTEN data with exon-4 dropping out.
12:48pm February 27th 2019 via Hootsuite Inc.
RT @nanostringtech: Our #SpatialGenomics Summit panel: Joe Beechem, PhD, NanoString, Fei Chen, PhD, The Broad, Jeffrey Moffitt, PhD, Boston…
Beechem #AGBT19 They had multiple different barcodes for the same mRNA probe - and thus every mRNA species have 10… https://t.co/lnCHwrhIe1
12:47pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Illustrates chemokine data at the RNA - a whole cascade by location and interaction.
12:46pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Points out poster #614 Friday at 440pm for their pathway analysis in space. Tumor center, invasive… https://t.co/kKpV7Kikp0
12:44pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Figure of probing the reactome. https://t.co/lVy1SLbrYn
12:43pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 High-profile Nature Med Nov 2018 https://t.co/hdS1boNxBN Three centers of excellence - Holland, OHS… https://t.co/kJOlr51rLr
12:42pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 (Correction - with PD-L1 on back of CD68.) Unusual microscope - integrates with clinical informatio… https://t.co/inyKHzQz4A
12:40pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Work with David Rimm (Yale) looking at tumor microenv in melanoma, 44 proteins across 59 patients.… https://t.co/Qo8IxVIRpH
12:38pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Now you get 100% cellularity of the tumor. The mask is inverted - just like Photoshop 'at single-cell resolution'.
12:36pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Shows a CRC sample, with PanCK in yellow (tumor) and CD45+ (immune cells in microenvironment). Show… https://t.co/UwyCJynnVa
Beechem #AGBT19 Needed dual digital micromirror chips, each with 1M mirror features. Can configure to any tissue geometry.
12:34pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 35-50bp of RNA, UMI's, spatial indexes, RNA ID index. Readout on any ILMN instrument.
Beechem #AGBT19 explaining how GeoMx DSP works. https://t.co/W0KiZacxem
12:33pm February 27th 2019 via Hootsuite Inc.
Here at #AGBT19 @nanostringtech putting on a great Spatial Genomics workshop just getting started (Palms Ballroom) https://t.co/TBgxttySnS
12:32pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Affinity reagent mix; read-out by moving scope (guided by colors) then cleave spec area. Sip it up… https://t.co/C5mca6t2zi
12:31pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 At Probes, dev Alexa dyes, putting assay onto tissue, 10 answers. The insight: take probes or Abs -… https://t.co/NVGRZTCfoa
12:30pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Now back together - GeoMx DSP with NGS readout here. The technology: first with protein.
12:28pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Back in the lab - things worked better than hoped. Own history - Joe started at Hopkins, onto Vande… https://t.co/Oql65jgcb5
12:27pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Shows a slide of Gordon Mills (then with MD Anderson, now OHSU). 'How do you invent a digitial spat… https://t.co/WjXQ2c7sq5
12:26pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 How invented: 1412 genes, 5000 indep RNA probes, spatially-resovled in a single-step rcn with FFPE… https://t.co/nP5iXruF5s
12:25pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 Discovery and translation - 500M (est) FFPE slides on earth (BMC Med Genomics '09) "'Room' for everybody' - early days.
12:24pm February 27th 2019 via Hootsuite Inc.
Beechem #AGBT19 A 'non-identifiability' problem - you can't put the spatial-axis back together after it has been de… https://t.co/t6BUCDPCnO
12:22pm February 27th 2019 via Hootsuite Inc.
Joe Beechem (Nanostring) at #AGBT19 Digital Spatial profiling- Delivery on the promise of Spatial Genomics
12:21pm February 27th 2019 via Hootsuite Inc.
MT @EricTopol: A reflection back on the #NHS review (https://t.co/gxwYR4Pccx) and some differences between #US and… https://t.co/iUFNXQjUIA
12:05pm February 27th 2019 via Hootsuite Inc.
Great to be back at Marco Island for #AGBT19 - what a view! https://t.co/6SSXlqiLWJ
12:02pm February 27th 2019 via Hootsuite Inc.
RT @coregenomics: And here's how @illumina @RocheSequencing @iontorrent @PacBio @BGI_Genomics @CompleteGenomic and… https://t.co/sKla2iri4G
11:05am February 27th 2019 via Hootsuite Inc.
How to Delete Your Data From 23andMe, Ancestry, and Other Sites | Consumer Reports https://t.co/Ud71T29QXX
10:31am February 27th 2019 via Buffer
RT @EricTopol: A ★ superb review of wearable biosensors to detect chemistry analytes in body fluids, including inte… https://t.co/cs9bWj61tm
10:10am February 27th 2019 via Hootsuite Inc.
RT @Dereklowe: Targeting microRNA species with small molecules - it can indeed be done, but it's still a wild front… https://t.co/fAiaSgH4Ca
10:05am February 27th 2019 via Hootsuite Inc.
RT @EricTopol: Months after #CRISPR human embryo genome editing so many serious questions linger… https://t.co/gchhTPrb7x
9:55am February 27th 2019 via Hootsuite Inc.
RT @NatureNews: Where, and under what circumstances, another gene-edited baby could be conceived is anyone’s guess.… https://t.co/U5EEp7sj6Q
9:10am February 27th 2019 via Hootsuite Inc.
Neat 45' audio interview with George Church. WGS should be zero dollars, encrypted, and like a seatbelt: needed for… https://t.co/UB1iWIjMCH
7:15am February 27th 2019 via Hootsuite Inc.
RT @ara_anderson: .@NCIDirector discusses the 4 major focus areas he will work on during his time as director. https://t.co/hpHfD18rhh
6:31am February 27th 2019 via Hootsuite Inc.
RT @AlbertVilella: @DaleYuzuki Drop-seq is one of the methods in the SingleCell omics table here https://t.co/5PNIfjDkZZ
6:07am February 27th 2019 via Hootsuite Inc.
New post for #AGBT19 Dolomite Bio – Drop-Seq for single-cell genomics https://t.co/E3ACXB7siG
5:05am February 27th 2019 via WP to TWTR on Yuzuki.org
a16z Podcast: What’s in the Water at the George Church Lab? | Andreessen Horowitz https://t.co/xMChwrpkKe
10:03pm February 26th 2019 via Buffer
Thermo Fisher Scientific Oncomine Dx Target Test Gets Expanded Regulatory Approval in Japan | GenomeWeb https://t.co/5XSznFW8X8
5:17pm February 26th 2019 via Buffer
