Woolly mammoth on verge of resurrection, scientists reveal | The Guardian https://t.co/L4jQ8PYiuc
11:15am February 17th 2017 via Hootsuite
DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification | Science https://t.co/7fQlDUwRnU
10:02am February 17th 2017 via Hootsuite
RT @coregenomics: #agbt17 Tweeters... @DaleYuzuki @jgreid @tysonaclark @mike_schatz @mason_lab @Supergecko and me! https://t.co/S5MMUlOZqB
6:41pm February 16th 2017 via Hootsuite
The Next Pseudoscience Health Craze Is All About Genetics | Gizmodo https://t.co/J0INorTkRu
5:33pm February 16th 2017 via Hootsuite
Thanks for all the great discussion #AGBT17 - hope to see you next year!
4:15pm February 16th 2017 via Hootsuite
Green: #AGBT17 The first Precision Health meeting was an experiment; all invited to the 2nd year Sept 14-16 in Scottsdale AZ
4:14pm February 16th 2017 via Hootsuite
@mason_lab @M_L_Carpenter @TorontoGenomics @coregenomics @dgmacarthur @obahcall Alas headed back to DC right away. :(
4:13pm February 16th 2017 via Hootsuite in reply to mason_lab
Q: Live cells? Daugherthy: Something they are thinking about; toxicity and getting probes into cells will be difficult #AGBT17
4:09pm February 16th 2017 via Hootsuite
Q: Viral sequences? Daugherthy: Doing that work now supported by Gates #AGBT17
Here's an interesting Vimeo of Readcoor's technology (with George Church) https://t.co/vUikIn98CZ #AGBT17
4:05pm February 16th 2017 via Hootsuite
RT @coregenomics: ED: maybe run a cool workshop like @nanostringtech can I be the guineapig again? @readcoor #agbt17 https://t.co/4B9CCHz6lI
4:04pm February 16th 2017 via Hootsuite
Daugherthy: Goal is to look at DNA, RNA, protein. Announces: Human Cell Atlas https://t.co/INtbrkT7Cl and Precipitatebio #AGBT17
4:02pm February 16th 2017 via Hootsuite
Daugherthy: Est they can now detect about 10K unique molecules/cell. Are developing automation. Show prelim data of DNA, protein #AGBT17
3:59pm February 16th 2017 via Hootsuite
Daugherthy: Can find biologically meaningful base change data; gives example of intentional wounding and a healing signature #AGBT17
3:54pm February 16th 2017 via Hootsuite
Daugherthy: Then RCA, called a 'rolony' - representing sequence plus position #AGBT17. RNA-FISSEQ is RNA-Seq data.
3:51pm February 16th 2017 via Hootsuite
Daugherthy: Shows impressive 3D video (with colors!) nice visuals. https://t.co/ME0388QEca Fix RNA, RT primer, extend, circularize #AGBT17
3:49pm February 16th 2017 via Hootsuite
Daugherthy: FISSEQ - spatial information rather than just a list. Original Church Science paper '14 https://t.co/YGFIAyrpvi #AGBT17
3:46pm February 16th 2017 via Hootsuite
Evan Daugherthy (Harvard Univ MA) A fully automated massively-multiplex panomic in-situ sequencing #AGBT17
3:45pm February 16th 2017 via Hootsuite
RT @shgoodwin1: Last point - in bold - in Rosenfeld talk. Be carefull where you buy sperm. Only at #agbt17
3:43pm February 16th 2017 via Hootsuite
RT @morgantaschuk: The amazing NASA biomolecule sequencer patch that @mason_lab showed at #agbt17 https://t.co/IZdHfpr8vp
Rosenfeld: Used 10X method; of 8 cases, 8 controls, only got 5 cases and 3 normals. Evidence of 65K sperm from 1 sample #AGBT17
3:35pm February 16th 2017 via Hootsuite
Rosenfeld: Shares feedback of sperm banks regarding cases of autism. Shows donor sibling registry, anonymizing. 50 to 100 kid/donor #AGBT17
3:33pm February 16th 2017 via Hootsuite
Rosenfeld: Why: haploid; easy to obtain; only biological contribution from the father; can determine raw mutation rate. #AGBT17
3:30pm February 16th 2017 via Hootsuite
Jeff Rosenfeld (Rutgers Univ NJ) Single-cell RNA sequencing in sperm from fathers of autistic children #AGBT17
3:29pm February 16th 2017 via Hootsuite
Nolan: Setup a company called Apprise Bio to move the technology forward. https://t.co/7s1vCECmWW #AGBT17
3:26pm February 16th 2017 via Hootsuite
Nolan: Barcoding 10's of million cells, can be barcoded in a few hours. Ab reads are better than FACS, equiv to CyTOF; DNA and RNA #AGBT17
3:25pm February 16th 2017 via Hootsuite
Nolan: Ultra-rare cell seq: (1 in a million). You need is one known 'hook'. PCR hook with known, cell barcode can go back an pull #AGBT17
3:24pm February 16th 2017 via Hootsuite
Nolan: New RNA method: proximity ligation approach, multiple RNA targets. (Called 'Snail'.) #AGBT17
3:22pm February 16th 2017 via Hootsuite
Nolan: Shows replicate data, CD44 x TCRbeta. 50K cells 'by sequencing' the Ab tags, 30 markers at a time. #AGBT17
3:21pm February 16th 2017 via Hootsuite
Nolan: Additive splints, combinatorial, then you can do a flow standard and look for co-expression patterns #AGBT17
Nolan: Able to get cells tagged with individual DNA sequences. Split pool synthesis - each cell goes through from plate to plate... #AGBT17
3:19pm February 16th 2017 via Hootsuite
Nolan: Ab panels have problems, fluorescence is 'contaminated', instruments are variable. Have DNA tags on Abs #AGBT17
3:18pm February 16th 2017 via Hootsuite
Nolan: '17 Cell https://t.co/XhWwW45SMM shows feature of cells ordered by connectivity of 'only 50 parameters' via CyTOF #AGBT17
3:17pm February 16th 2017 via Hootsuite
Gary Nolan (Stanford Univ CA) Sequencers as flow cytometers: ultra-high throughput single cell analysis by split-pool synthesis #AGBT17
3:15pm February 16th 2017 via Hootsuite
Q: Pipette tis? Mason: Individually wrapped! #AGBT17
2:53pm February 16th 2017 via Hootsuite
Mason: Journey to Mars? Space-Seq open as a NASA resource in late '17. >200K isoforms observed from space travel #AGBT17
2:52pm February 16th 2017 via Hootsuite
Mason: Finishes with the need for standards, SEQC2. Deep sequencing of the GIAB samples, putting base mods out, and ABRF studies #AGBT17
2:51pm February 16th 2017 via Hootsuite
Mason: Looking at m6A calling, mCaller Github repo: https://t.co/T8KssEnBsY #AGBT17
2:50pm February 16th 2017 via Hootsuite
RT @jaredtsimpson: Slides from my #AGBT17 talk: https://t.co/UgmjkWXPwZ
2:48pm February 16th 2017 via Hootsuite
Mason: 2D reads 89-92%; 1D reads 76-7%. About 1-2% better than ground-data. De-novo on Amazon. Perhaps do assy in space? #AGBT17
2:47pm February 16th 2017 via Hootsuite
Mason: v7 flow-cells, loaded library, shows video of data coming off, great photo https://t.co/dPiSzvety7 #AGBT17
2:45pm February 16th 2017 via Hootsuite
Mason: Photos of @Astro_Kate7 and rocket blows up, gets a nice letter 'saying your supply ship blew up'. Kate gets up there in Aug #AGBT17
Mason: Nanopore in Antarctica, Africa. What about space? Cool patch shown. $10K/kg upmass cost. Preprint https://t.co/VL70HHtldj #AGBT17
2:44pm February 16th 2017 via Hootsuite
Mason: continuing with urban metagenomics, https://t.co/HL2K8Of6bD looking at ubiquitous sequencing https://t.co/p94W4CT1PV #AGBT17
2:42pm February 16th 2017 via Hootsuite
Mason: ...some allele-spec switching of gravity response genes in RNA-seq data #AGBT17
2:41pm February 16th 2017 via Hootsuite
Mason: Cells were alive but 'not happy' due to handling / landing of spacecraft. Looking at allele-spec expression (ASE) shows... #AGBT17
2:40pm February 16th 2017 via Hootsuite
Mason: Terrestrial vs ISS RNA-Seq; mtDNA reads map when there are many dying cells. Blood drawn in space and sent back via Soyuz #AGBT17
Mason: #AGBT17 Suggested that this approach https://t.co/lgCsYcK6rO could be used in combination with ONT, some data so far.
2:39pm February 16th 2017 via Hootsuite
Mason: Nature News piece https://t.co/2jH0BgtTKU Looked at telomere length; RFI ovia FISH of T-cells; no easy way to validate #AGBT17
2:37pm February 16th 2017 via Hootsuite
Mason: #AGBT17 (Is trying to talk as fast as he can about the two-year NASA twin study.) NGS can 'confirm they are identical'.
2:36pm February 16th 2017 via Hootsuite
