Best: Showed data for ribosome binding site enrichment. SWARM-enhanced - for and optimum classifier (nice video of birds swarming) #Tricon
5:55pm February 20th 2017 via Hootsuite
Best: Number of spliced RNAs in platelets numbered above 4500. Determined sensitivity of 10-50M reads/sample sufficient #Tricon
5:51pm February 20th 2017 via Hootsuite
Best: Stg II-IV matching for age, smoking status, blood storage time for lung ca. 263 matched #Tricon
5:50pm February 20th 2017 via Hootsuite
Best: Low sample numbers; EDTA-storage time; non-age-matched cohorts; effect of inflammation. Thus 700 prospective smpls #Tricon
5:49pm February 20th 2017 via Hootsuite
Best: Showed algorithmic training, with training and test set. From their '15 ref https://t.co/iOgW2l1Mnm several comments #Tricon
5:48pm February 20th 2017 via Hootsuite
Best: Tumor-educated platelets (TEP) splice mRNAs. Able to show pan-cancer dx via TEP-derived spliced RNA gene panels #Tricon
5:47pm February 20th 2017 via Hootsuite
Best: #Tricon This first paper on platelet RNA https://t.co/mor4q62qSb led to this paper, a method thromboSeq https://t.co/U4jzzrlHtR
5:45pm February 20th 2017 via Hootsuite
Best: Points to this '14 ref and platelet's role in metastasis https://t.co/YBwanfKaoJ Platelets sequester RNA #Tricon
5:43pm February 20th 2017 via Hootsuite
Myron Best (VU Medical Center Amsterdam) Tumor-educated platelets as a blood-based liquid biopsy platform for cancer dx #Tricon
5:41pm February 20th 2017 via Hootsuite
Pantel: Shows great fig from '16 Clin Chem rev https://t.co/7W4FM22VLa about purposes and complementarity bet ctDNA and CTCs #Tricon
5:39pm February 20th 2017 via Hootsuite
Pantel: Also comparison of methodologies, dev of db's, dev of blood-based CDx and new drugs #Tricon
5:37pm February 20th 2017 via Hootsuite
Pantel: Has organized a consortium called CANCER-ID with dozens of organizations; https://t.co/rGIacrIpcs want SOPs for biobanking #Tricon
5:36pm February 20th 2017 via Hootsuite
Mouritzen: Exosomes are not only biomarker-containing, but also have biological function '15 Nature https://t.co/cUdEfiy7Ge #Tricon
5:34pm February 20th 2017 via Hootsuite
Pantel: Even platelets contain cancer-specific information '15 cancer cell https://t.co/idPCbqXStE #Tricon
5:33pm February 20th 2017 via Hootsuite
Klaus Pantel (Hamburg-Eppendorf Germany) New kits on the block for liquid biopsy #tricon
5:32pm February 20th 2017 via Hootsuite
Q: Any work on clin trial response monitoring? Mouritzen: No Q: Stat. treatment for signal? A: No normalization, paper in prep #Tricon
4:42pm February 20th 2017 via Hootsuite
Mouritzen: With and w/o exosome isolation, less powerful ROC, nonethless grey-zone group AUC 0.907, sens 88%, spec 95%. #Tricon
4:36pm February 20th 2017 via Hootsuite
Mouritzen: Then 227 individuals etc. currently in validation. Showed ROC curve of disocvery - Sens 88%, spec 95%. AUC 0.955 #Tricon
4:35pm February 20th 2017 via Hootsuite
Mouritzen: A full 'miR-ome': 752 miRNAs in 50 individuals; screen 259 w/188 subset; then 222 w/22 BPH, 200 PCa w/ 92 miR subset #Tricon
4:33pm February 20th 2017 via Hootsuite
Mouritzen: Their test - <2h from 3mL of urine, 20 min isolation; in discovery phase, have ID'd a 3-miRNA signature #Tricon
4:32pm February 20th 2017 via Hootsuite
Mouritzen: Thus Pr ca is overtreated; 2.3M biopsies; 1.5M could be avoided. #Tricon
4:31pm February 20th 2017 via Hootsuite
Mouritzen: And 7/10 are False Positives; 1/10 do have cancer; PSA grey-zone of 3-10ng/mL very low pred value (25%) 40% this zone #Tricon
Mouritzen: miRNA case study of pr ca in urine: reviews weakness of PSA test. 45M tests/yr, >1M new cases/yr (600K in EU and US) #Tricon
4:30pm February 20th 2017 via Hootsuite
Mouritzen: Shows nice concordance between their LNA qPCR system quant of fold-change to NGS data across >10 species of miRNAs. #Tricon
4:28pm February 20th 2017 via Hootsuite
Mouritzen: (Ratio of NGS:overlap:qPCR - NGS is isomiRs and not present on panels) #Tricon
4:27pm February 20th 2017 via Hootsuite
Mouritzen: When unbound by TPM (tags per million reads), about 900 spp of miRNAs detected. Shows Venn of NGS vs qPCR: 347:348:109 #Tricon
4:26pm February 20th 2017 via Hootsuite
Mouritzen: Able to get >80% mappability. 25% miRNAs; reviews pro's and con's of qPCR cp to NGS; hypothesis vs. hypothesis-free #Tricon
4:25pm February 20th 2017 via Hootsuite
Mouritzen: Also dev RNA spike-ins for monitoring cDNA synthesis, isolation efficiencies, and lib prep efficiency (52 spp of spikes) #Tricon
4:20pm February 20th 2017 via Hootsuite
Mouritzen: Thus specialized pur kits, LNA-based qPCR, and optimized lib preps #Tricon
4:19pm February 20th 2017 via Hootsuite
Mouritzen: Limited amts of RNA; PRC inhibitors; cellular contam. and hemolysis are challenges for microRNAs #Tricon
Mouritzen: Stability due to assoc'n w/exosomes fig from '07 ref https://t.co/IxN0UbJOvv #Tricon
4:18pm February 20th 2017 via Hootsuite
Mouritzen: microRNAs are 'excellent diag biomarkers' due to exosomes, stability, biological potential, limited number #Tricon
4:16pm February 20th 2017 via Hootsuite
Molecular #Tricon 2017 starting wtih Exiqon Peter Mouritzen RNA in Liquid Biopsies - promising new biomarkers of diseases
4:15pm February 20th 2017 via Hootsuite
New Zealand is the tip of a ‘hidden continent.’ - The Washington Post https://t.co/iQl63tRS8u
2:10pm February 20th 2017 via Hootsuite
How mammoth cloning became fake news – Medium https://t.co/fb4pXrkUcj
1:05pm February 20th 2017 via Hootsuite
MT @Xtalks: Enabling Clinical #Genomics with Highly Multiplexed and Patient-like Reference Materials w/ @SeraCare https://t.co/X6hSpBQ4WX
11:30am February 20th 2017 via Hootsuite
Forget genius. Science is the product of less-than-brilliant minds - LA Times https://t.co/PwV8lmqoRq
11:10am February 20th 2017 via Hootsuite
Cautious Optimism (Dec 2015): Lessons from the Cognitive Front Lines: Early Adopters of IBM’s Watson WSJ https://t.co/EL1PMtPYDm
10:46am February 20th 2017 via Hootsuite
RT @DrKhouryCDC: Can solid tumor genome sequencing ID mutations that improve clinical outcomes? Via @PLOSMedicine https://t.co/qOxEf2XX0K
10:39am February 20th 2017 via Hootsuite
Background on Watson's AI: Why go to the Moon when what your company really needs is in the Rockies? Kyield https://t.co/W5kkevdCuw
10:02am February 20th 2017 via Hootsuite
MD Anderson and IBM Watson's Oncology Expert Advisor: a $62M investment in healthcare AI goes sour | Forbes https://t.co/bX8chPC65j
9:29am February 20th 2017 via Hootsuite
Post Edited: Bionano Genomics goes human-genome scale https://t.co/8fUHkUl4li
8:26pm February 18th 2017 via WP to TWTR on Yuzuki.org
A survey of 53 attendees at #AGBT17: what caught your attention? @DeciBio https://t.co/txlMzFMVWo
7:19pm February 18th 2017 via Hootsuite
For all you at #AGBT17 RT @DaleYuzuki: New post: Bionano Genomics goes human-genome scale https://t.co/8fUHkUl4li
7:11pm February 18th 2017 via Hootsuite
New post: Bionano Genomics goes human-genome scale https://t.co/8fUHkUl4li
7:10pm February 18th 2017 via WP to TWTR on Yuzuki.org
Prepare to Obsess Over the Future of Home Wifi | Medium https://t.co/qflXKPUYW8
3:22pm February 18th 2017 via Hootsuite
Beware, the content goes deeeeep... RT @OmicsOmicsBlog: #AGBT17 Tweet Archive is Up! https://t.co/frBl0z0oLG
8:46am February 18th 2017 via Hootsuite
RT @NatureNews: Four reasons the CRISPR patent verdict isn’t the end of the story. https://t.co/gU0tdPZD7Q https://t.co/cefWo1ZLpe
5:10pm February 17th 2017 via Hootsuite
MT @moorejh: Mining the Human Gut Microbiota for Immunomodulatory Organisms - Cell https://t.co/bPCNFFMVh1 https://t.co/HxDGBreNnN
4:10pm February 17th 2017 via Hootsuite
RT @NewsfromScience: The H7N9 avian flu is spreading in China: https://t.co/RqFX1GPUCH
2:57pm February 17th 2017 via Hootsuite
