Tweets from Hootsuite

Delamarche: Inkjet-based deposition of reagents, reagents dissolve, accumulate along flow direction. But: homogeneous dissolution? #MDxEU17

12:13pm April 12th 2017 via Hootsuite

Delamarche: Neat video shows subdivision into test areas 1/2/3/4. 1nL/sec or less. Can drive a test for 10' w/1uL of sample #MDxEU17

12:12pm April 12th 2017 via Hootsuite

Delamarche: Map flow resistance (analogy to electrical resistance calculations). Shows chip at 1.2nL/s and filling via capillary #MDxEU17

12:11pm April 12th 2017 via Hootsuite

Delamarche: '02 https://t.co/HtAxvgqRoX The flow rate is the capillary pressure divided the viscosity of the liquid. 50um - 100um #MDxEU17

12:10pm April 12th 2017 via Hootsuite

Delamarche: Fabrication and manufacturability is what they've done a lot of already. Smartphone and peripheral communication #MDxEU17

12:08pm April 12th 2017 via Hootsuite

Delamarche: Flow valves, sensors, bypass channels, bead trap arrays. Focus on autonomous flow generation, reagent integration #MDxEU17

12:08pm April 12th 2017 via Hootsuite

Delamarche: Developed a library of fluidic components: loading pads, capillary pumps, flow splitters, flow resistance, junctions #MDxEU17

12:07pm April 12th 2017 via Hootsuite

Delamarche: Also easy to counterfeit:. High-precision and portable immunodiagnostics: control conc, timing, mixing, multiplexing #MDxEU17

12:06pm April 12th 2017 via Hootsuite

Delamarche: But uses large sample vol; hard to optimize geometry of signal; multiplexing hard; qualitative; no flow control #MDxEU17

12:05pm April 12th 2017 via Hootsuite

Delamarche: Shows diagram of a lateral flow assay, detection Abs w/fluors or chemistry. Easy to use, low cost #MDxEU17

12:04pm April 12th 2017 via Hootsuite

Delamarche: POC Dx: security features (a worldwide fight), sample detection, assay protocol, data proc via smarphone #MDxEU17

12:03pm April 12th 2017 via Hootsuite

Delamarche: #MDxEU17 Shows fig. from '11 ref https://t.co/P7mvryAe35 Engineering is fine; but the microfluidics is the more difficult part

12:02pm April 12th 2017 via Hootsuite

Delamarche: Begins with two paintings from Jan Steen 'The Doctor's Visit' - one diagnostic (urine test) and therapeutic #MDxEU17

12:00pm April 12th 2017 via Hootsuite

Emmanuel Delamarche (IBM CH) Precision diagnostics based on modular capillary-driven elements and with smartphone connectivity #MDxEU17

11:59am April 12th 2017 via Hootsuite

Stahlberg: #MDxEU17 '16 NAR ref https://t.co/E5Fu18dlAt

9:58am April 12th 2017 via Hootsuite

Stahlberg: Target primer forms a long stem, a 'lollipop' primer design. (Recent Nature Prot ref https://t.co/e1zJ6igMwY ) #MDxEU17

9:57am April 12th 2017 via Hootsuite

Stahlberg: Illus w/SafeSeqS and non-spec products produced. In single-cell field, Hid barcode via stem-loop complementarity #MDxEU17

9:54am April 12th 2017 via Hootsuite

Stahlberg: Shows the effect of mutation via amplification. Illustrates barcoding via PCR before ligation: specificity an issue #MDxEU17

9:53am April 12th 2017 via Hootsuite

Stahlberg: Shows disgram of pt-spec mutations and their relationship with tumor burden; but a needle in a haystack problem. #MDxEU17

9:49am April 12th 2017 via Hootsuite

Anders Stahlberg (Gothenburg SW) Simple, multiplexed, PCR-based barcoding of DNA for ultra-sens mutation detection by NGS SiMSenSeq #MDxEU17

9:47am April 12th 2017 via Hootsuite

Thierry:A2: Here's one paper on preanalytic considerations '13 ref https://t.co/qJiDFm2UnC #MDxEU17

9:44am April 12th 2017 via Hootsuite

Q:What's the assumption? Larger fx assoc'd with cancer? Thierry: Preanalytic conditions 'really impt'. Many good papers ruined. #MDxEU17

9:42am April 12th 2017 via Hootsuite

Q: Size? Thierry: Feels an artifact of NGS, Agilent, gel, and qPCR. Feels his results are robust, confirmed. Primer design <100bp #MDxEU1

9:40am April 12th 2017 via Hootsuite

Thierry: "At 0.1%, are you missing 10% of the mutations?" Technology licensed to DiaDx to commercialize. CNAPS in Sept in France #MDxEU17

9:37am April 12th 2017 via Hootsuite

Thierry: Kplex R study published today - Clin Cancer Res https://t.co/uBe5hZBrGJ Submitted data shows frag index data, K-M curve #MDxEU17

9:35am April 12th 2017 via Hootsuite

Thierry: Recent ref https://t.co/fulcqy0CiB ctDNA is 'enough to follow tumor burden'. 'A strong prog tool' https://t.co/OctXnUJJvb #MDxEU17

9:31am April 12th 2017 via Hootsuite

Thierry: By having a sensitivity cut-off: may avoid pts for Rx. Discordance is up to 25%; with MDAnderson NTC00501410 for mCRC MDxEU17

9:29am April 12th 2017 via Hootsuite

Thierry: Here's a reference looking at AFM of ctDNA size by direct measurement https://t.co/zzhXKMYVEP #MDxEU17

9:24am April 12th 2017 via Hootsuite

Thierry: Just-in-time analysis: Kplex2 clinical trial, 140pts, 7 KRAS + BRAF V600E as blinded study. #MDxEU17

9:23am April 12th 2017 via Hootsuite

Thierry: This work '11 PLoS One https://t.co/bkB1djRIeE Shows enrichment of 60-100bp sizes. Dev "Intplex" - https://t.co/oVASPh9rCa #MDxEU17

9:19am April 12th 2017 via Hootsuite

Thierry: The smaller the size of the ccfDNA, the higher the amount by qPCR. Showed AFM imaging, with large proportion <100bp #MDxEU17

9:14am April 12th 2017 via Hootsuite

Alain Thierry (Montpelier INSERM) Circulating DNA analysis as the next-generation diagnostic in oncology #MDxEU17

9:13am April 12th 2017 via Hootsuite

Laurent-Puig:A2: May be due to capillary structure in liver. Pankel: A need to look at source of metastasis and ctDNA #MDxEU17

6:53am April 12th 2017 via Hootsuite

Q: Why for NSCLC so low ctDNA? Laurent-Puig: Have clinical correlation, high ctDNA is liver met., where it may be coming from #MDxEU17

6:52am April 12th 2017 via Hootsuite

Q: Is this in routine Dx? Laurent-Puig: They use NGS routinely, EGFR for lung ca. Next step: change treatment w/better prognosis #MDxEU17

6:51am April 12th 2017 via Hootsuite

Laurent-Puig: Chemo treatment eval ctDNA is indicator of efficacy. #MDxEU17

6:49am April 12th 2017 via Hootsuite

Laurent-Puig: Concl: presence of ctDNA in early stg ca post-surgery is indicator of early recurrance #MDxEU17

6:49am April 12th 2017 via Hootsuite

Laurent-Puig: ctDNA variation in lung ca between T0 and first eval. Shows PFS curves for amts of ctDNA https://t.co/KJvdUXTIkS #MDxEU17

6:40am April 12th 2017 via Hootsuite

Laurent-Puig: Shows hypermethylation data in ctDNA from this '16 ref https://t.co/laOoqHTLbQ WIF1, NFY markers #MDxEU17

6:36am April 12th 2017 via Hootsuite

Laurent-Puig: Follow-up is linked to ability to detect - shows limitation of dPCR by number of mutations that need to be tested #MDxEU17

6:34am April 12th 2017 via Hootsuite

Laurent-Puig: 400pts, proportion of adv cancer pts with less than 2% of ctDNA in plasma: lung 45%, panc 26%, colon 16% #MDxEU17

6:32am April 12th 2017 via Hootsuite

Pierre Laurent-Puig (Paris Decartes Univ FR) Circulating turmo DNA clinical applications #MDxEU17

6:31am April 12th 2017 via Hootsuite

Alix-Panabieres: 11th minimal residual disease in cancer - Montpelier in May #MDxEU17

6:30am April 12th 2017 via Hootsuite

Alix-Panabieres: Heard during acknowledgements: "Not a big group, but a girl group" (all female) Proud to participate CANCER-ID #MDxEU17

6:29am April 12th 2017 via Hootsuite

Alix-Panabieres: Exp of PD-L1 on CTCs - able to use add'l CellSearch channel to interrogate. https://t.co/VEtw2HN99r #MDxEU17

6:27am April 12th 2017 via Hootsuite

Alix-Panabieres: Finding cells that give rise to metastasis, detecting viable ones. Fn test using EPISPOT https://t.co/q7vk6nYWdW #MDxEU17

6:25am April 12th 2017 via Hootsuite

Alix-Panabieres: Why so hard - at least 286 CTCs detected by CellSearch before you can get one to form a cell line #MDxEU17

6:24am April 12th 2017 via Hootsuite

Alix-Panabieres: 165 pts, only got 1 to crow as cell line. Able to get add'l lines (8 more) from same pt after Rx and relapse #MDxEU17

6:23am April 12th 2017 via Hootsuite

Alix-Panabieres: Hierarchical clustering of expression, CTCs are distinct. Biomarker potential - DNA repair, metabolism genes #MDxEU17

6:22am April 12th 2017 via Hootsuite