Irena Barbulovic-Nad (Kapplex Canada) Digital microfluidics: sample preparation to detection #MDxEU17
4:48am April 13th 2017 via Hootsuite
Q: Comments on storage? Buffer, carrier, temp? Devonshire: Post-extract, 4C . Concern about freeze-thaws. #MDxEU17
4:41am April 13th 2017 via Hootsuite
Q: How to spike DNA in? Devonshire: Prepped working conc of ADH, at point of extraction (50uL, vortex, extract) #MDxEU17
4:39am April 13th 2017 via Hootsuite
Devonshire: Confidence in whole-process - measurement of CV from 5 to 20%. How can this help? Knowledge of extraction eff process #MDxEU17
4:38am April 13th 2017 via Hootsuite
Devonshire: Quant: 2% - 4.5% on the 5%; 0.5% to 1.1% for the 1%; dPCR higher consistently over TAm-Seq, about 1% or 0.2% more meas #MDxEU17
4:35am April 13th 2017 via Hootsuite
Devonshire: Outliers are 0.1% sample. Analytical sens: 5%/1% 6/6 samples pos; 0.1% 4/6 or less. #MDxEU17
4:33am April 13th 2017 via Hootsuite
Devonshire: Measured about 125 copies/dPCR at 5%; 30 copies of 1%; about 4-5 copies for 0.1%. Efficiency: from 40-60%. #MDxEU17
4:32am April 13th 2017 via Hootsuite
Devonshire: 80ng of HDx, 20K GE/mL; analyzed by dPCR 1/2, 2/2 via Inivata's TAm-Seq. #MDxEU17
4:31am April 13th 2017 via Hootsuite
Devonshire: Worked with HDx with their synthetic cfDNA, shows their sonicated material; spiked in pooled plasma the synthetic #MDxEU17
4:30am April 13th 2017 via Hootsuite
Devonshire: (Total yield on the order of 70-80% though.) Whole process QC: looking from extraction through analytical, quant #MDxEU17
4:29am April 13th 2017 via Hootsuite
Devonshire: Jena's binding allows smaller <100bp. Measured via qPCR to eval % - ~10% of 115bp in larg fx; 8% of 461bp in smaller #MDxEU17
4:28am April 13th 2017 via Hootsuite
Devonshire: Shows results from five kits. Analytic Jena has a Polymer Mediated Enrichment (PME) for DNA sizing #MDxEU17
4:26am April 13th 2017 via Hootsuite
Devonshire: Points to several CSF papers and their utility https://t.co/hKdrhVWzCf Shows ADH spike efficiencies #MDxEU17
4:25am April 13th 2017 via Hootsuite
Devonshire: Showed ADH spike-in by size (115, 461 and 1448 bp spike-ins, strange yield was consistent w/larger frags) #MDxEU17
4:22am April 13th 2017 via Hootsuite
Devonshire: #MDxEU17 Used an ADH plasmid from Arabidopsis '13 PLoS One https://t.co/duiOBCse5K Used to eval extraction method, frag size
Devonshire: What if your yield is 2K GE, but you need 5K GE? Repeat extraction or get another sample? External control useful #MDxEU17
4:20am April 13th 2017 via Hootsuite
Devonshire: dPCR no calibration; shows side-by-side comparison of TERT/RPPH1 - higher quant in qPCR levels. #MDxEU17
4:19am April 13th 2017 via Hootsuite
Devonshire: Suggests using more than one gene is useful for quant yield. qPCR vs dPCR: qPCR scales to vol of sample, no dead vol #MDxEU17
Devonshire:Eval of yield - qPCR or dPCR either single-copy, or LINE-1/Alu rep seq. their '14 Anal Chem ref used TERT/ERV3/RPPH1 #MDxEU17
4:18am April 13th 2017 via Hootsuite
Devonshire: Interesting fig showing comparison of methods and impact on detection for ctDNA '16 https://t.co/pdePUJfIvr #MDxEU17
4:17am April 13th 2017 via Hootsuite
Devonshire: 0.1% is 1 copy in 1K GE; need min yield. Frag profile: cf fetal DNA is smaller than maternal; ctDNA is shorter lived #MDxEU17
4:14am April 13th 2017 via Hootsuite
Devonshire: Tumor component of course is the minor component. Showed fig from '14 ref https://t.co/dFHgathWjw from 3 kits via Alu #MDxEU17
4:13am April 13th 2017 via Hootsuite
Devonshire: cfDNA has 5-10 ng/mL in healthy plasma (1.5k-3k haploid genome equiv - GE) lower in other samples (CSF) #MDxEU17
4:12am April 13th 2017 via Hootsuite
Devonshire: Looking at extraction perf perameters; sample QC (haploid GE and efficiency); process QC (size profile, whole process) #MDxEU17
4:11am April 13th 2017 via Hootsuite
Alison Devonshire (LGC UK) Impact of extraction on cfDNA analysis: considerations of sample and process QC #MDxEU17
4:09am April 13th 2017 via Hootsuite
The Dark Secret at the Heart of AI - MIT Technology Review https://t.co/CKHVClofq7
5:56pm April 12th 2017 via Hootsuite
Can A Simple Blood Test Really Spot Cancer Early? Don’t Bet On It Yet, Scientists Say.| Buzzfeed https://t.co/hecLWaVM3L
2:00pm April 12th 2017 via Hootsuite
Bachmann: Looking at manufacturability, '16 ref https://t.co/8D175z4t15 #MDxEU17
12:54pm April 12th 2017 via Hootsuite
Bachmann: #MDxEU17 Detection of MRSA on this platform '13 ref https://t.co/eOJ8gn7GI9
12:50pm April 12th 2017 via Hootsuite
Bachmann: Towards AMR - New Delhi Metallolactamase synth target '15 ref https://t.co/m1YiPp3L2A Also plasmid#MDxEU17
12:48pm April 12th 2017 via Hootsuite
Bachmann: Surprising - binding to surface probe. 50pM LoD. Able to look at kinetics. https://t.co/WqzeMViOnY #MDxEU17
12:46pm April 12th 2017 via Hootsuite
Bachmann: PCR-free bacterial spp detection: try to remove as much complexity as poss. No PCR, target 16S. 6.8K-72K 16S rRNA/cell #MDxEU17
12:44pm April 12th 2017 via Hootsuite
Bachmann: A functionalized surface, EIS platform biosensing platform '06-'09. Chronic woundcare '09-'10. #MDxEU17
12:43pm April 12th 2017 via Hootsuite
Bachmann: Mentions Longitude Prize for the same goal. Their technology: electrochemical impedance spectroscopy, label free, simple #MDxEU17
12:42pm April 12th 2017 via Hootsuite
Bachmann: #MDxEU17 US FDA has named 10 semi-finalists, $50K apiece https://t.co/WAQc06Q17k
12:40pm April 12th 2017 via Hootsuite
Bachmann: #MDxEU17 Horizon Prize results page: https://t.co/PiFHI0Nqvh
12:39pm April 12th 2017 via Hootsuite
Bachmann: Many devices - Cepheid, Biocartis, Nanosphere, LIAT, optigene, Curetis. Challenge prizes - Philips Minicare HNL #MDxEU17
12:38pm April 12th 2017 via Hootsuite
Bachmann: What antibiotics can I use? Must not use? Want sample in, result out. Disposable cartridge, reusable reader #MDxEU17
12:37pm April 12th 2017 via Hootsuite
Bachmann: #MDxEU17 Mentions O'Niell report https://t.co/lKMVdueUZC Now has a joint programming initiative. https://t.co/sog0a9CcAJ
12:36pm April 12th 2017 via Hootsuite
Bachmann: WHO priority pathogen list for R&D of novel antibiotics; Davos in '16 and '17 for new diagnostics for AMR #MDxEU17
12:33pm April 12th 2017 via Hootsuite
Bachmann: Countries affected by AMR are the least prepared; Africa, Asia. Global policy response - UN General Assy #MDxEU17
12:32pm April 12th 2017 via Hootsuite
Bachmann: AMR - by 2050 10M deaths. Challenged: PLoS Med '16 https://t.co/pC0IQqQXiZ #MDxEU17
12:31pm April 12th 2017 via Hootsuite
Bachmann: His background is biosensors; mission is intersection of medical microbiology, bacterial diagnostics, microchips #MDxEU17
12:30pm April 12th 2017 via Hootsuite
Till Bachmann (Univ Edinburgh UK): Rapid diagnostic tests to tackle antimicrobial resistance #MDxEU17
12:29pm April 12th 2017 via Hootsuite
Q: Cp to MaxPix from Luminex? Delamarche: Simplicity of device; multiplexing in separate area. Portable; more precise #MDxEU17
12:28pm April 12th 2017 via Hootsuite
Delamarche: Finishes with very busy slide of the multitude of POC devices on the market today #MDxEU17
12:26pm April 12th 2017 via Hootsuite
Delamarche: Flow monitoring using capacitive sensing - human serum, PBS + 1% BSA, even DI water. Shows video #MDxEU17
12:25pm April 12th 2017 via Hootsuite
Delamarche: Shows nice electron micrograph of Ab-conjugated beads, and how they become trapped in the readout. PSA at 3pg/mL #MDxEU17
12:21pm April 12th 2017 via Hootsuite
Delamarche: Picogram qtys of reagents with nanoliter precision. Able to work with 10um beads as carriers for receptors #MDxEU17
12:19pm April 12th 2017 via Hootsuite
Delamarche: (The reagents accumulate at the front meniscus. Used orthogonal flow module for mixing.) Laid down trenches - barriers #MDxEU17
12:14pm April 12th 2017 via Hootsuite
