Gray: Dealing with two genomes: the germline and somatic; can unearth cancer susceptibility in germline as well. #Tricon
1:21pm February 21st 2017 via Hootsuite
Gray: Providers struggle to keep up with 'rapidly evolving science'; the microenvironment. Understanding of tumor evolution #Tricon
1:20pm February 21st 2017 via Hootsuite
Gray: Points out that patients are not populations. Uses example of PD1 https://t.co/imUKGtiGQl Inadequate genomic knowledge in pop #Tricon
1:19pm February 21st 2017 via Hootsuite
Gray: Starts with the Wagle melanoma figure from this '11 ref https://t.co/86QkdvNpCf #Tricon
1:17pm February 21st 2017 via Hootsuite
Stacy Gray (City of Hope, CA) Precision cancer medicine at the bedside - the provider perspective #Tricon
1:15pm February 21st 2017 via Hootsuite
Karin: Mouse IgA knockouts - enhances CR carcinogenesis in mice. Shows fecal seq in IgA-deficient mice - a battle bet bad/good bact #Tricon
11:43am February 21st 2017 via Hootsuite
Karin: Now moves onto secretory IgA - mg's secreted into the feces daily. https://t.co/cuXSnsrtcL PIgR https://t.co/967c5rb4tg #Tricon
11:39am February 21st 2017 via Hootsuite
Karin: When APC is lost, has data showing loss of JAM-A and MUC2 proteins, losing the tight junction barriers between colon cells #Tricon
11:33am February 21st 2017 via Hootsuite
My new title is 'Super Tweeter' (I think I'll update my profile...) https://t.co/YTL5ycD9fX
11:31am February 21st 2017 via Hootsuite
Karin: Shows 16S RNA FISH of CR tissues: assoc'd with localized permeability to microbes and/or their products #Tricon
11:29am February 21st 2017 via Hootsuite
Karin: Shows data where IL-23 signaling promotes CR ca, T-assoc macrophages and NF-kB producing IL-23: look for IL-23 inducer #Tricon
11:27am February 21st 2017 via Hootsuite
Karin: Inactivating APC in mice, look at IL-23 and IL-17 elevation, upregulated in spontaneous colon ca https://t.co/1GigszIrLb #Tricon
11:25am February 21st 2017 via Hootsuite
Karin: Inflammatory genes IL-23 etc expression associated with poor prognosis https://t.co/lgdz4TZ9qg #Tricon
11:23am February 21st 2017 via Hootsuite
Karin: APC in colitis-assoc'd doesn't show up early (it's a gatekeeper) until much later. Colitis assoc'd: cytokines IL-23, IL-17 #Tricon
11:21am February 21st 2017 via Hootsuite
Karin: The key is to detect early, and treat early. First target - colitis-assoc'd cancer (i.e. Crohn's, IBD). #Tricon
11:19am February 21st 2017 via Hootsuite
Karin: CR ca: in 70% of cases follows a pre-defined pathway. '10 review of inflammation https://t.co/uOQjptfmTw Only 10% 5y OS StgIV #Tricon
Karin: Then chemokines, cytokines recruit lymphocytes, can create oxidation and other effects that can cause cancer #Tricon
11:17am February 21st 2017 via Hootsuite
Karin: C-R ca is 2nd or 3rd leading cause of death. Unified theory of inflammation: response to injury, pathogens or flora invade #Tricon
11:16am February 21st 2017 via Hootsuite
Michael Karin (UCSD SoM CA): Tumor elicited inflammation in colorectal cancer #Tricon
11:15am February 21st 2017 via Hootsuite
Powell: Can detect below 0.1% mutant via qPCR or NGS, exhibiting here at #Tricon
11:14am February 21st 2017 via Hootsuite
Powell: Doing a clinical study in China, w/Zhejiang Univ and John Radcliffe Hosp (Bodmer) #Tricon
11:13am February 21st 2017 via Hootsuite
Powell: For Coloscape, 4 genes and 20 mutations; for early c-r ca, 62% sens in their preliminary studies. Picking up early muts #Tricon
11:12am February 21st 2017 via Hootsuite
Powell: Coloscape - is a kit to provide to hospital labs, unlike Cologuard's central lab model. Cologuard sens for pre-ca is 41% #Tricon
11:11am February 21st 2017 via Hootsuite
Powell: XNA removes all wild-type background noise. Shows KRAS w/ and w/o Qclamp. Have done liquid biopsy, shown concordance #Tricon
11:10am February 21st 2017 via Hootsuite
Powell: Also Dimer removal for NGS, and a Colon test. XNA is a neutral backbone, salt-neutral; if there's a mutant, product will amp #Tricon
11:09am February 21st 2017 via Hootsuite
Powell: XNA is a blocking oligo; they have tech for qPCR, for NGS called OptiSeq, apply it for CRISPR editing event-screening #Tricon
11:08am February 21st 2017 via Hootsuite
Michael Powell (Diacarta CA) Next-generation precision molecular diagnostics powered by XNA #Tricon
11:07am February 21st 2017 via Hootsuite
Volker: He's also a cautionary tale of how sequencing needs to be done as quickly as possible. Ultimate goal - baby sequencing #tricon
8:48pm February 20th 2017 via Hootsuite
Volker: The goal of the foundation is to make it more available around the world. Worthey: Many organizations raising funds #Tricon
8:47pm February 20th 2017 via Hootsuite
Worthey: Things have moved fast, but still a shocker that it still isn't more common. The evidence is all there. #Tricon
8:46pm February 20th 2017 via Hootsuite
Volker: Cured of two diseases, but PTSD, Tuorette's, potential autism today. If he presented now, he could be sequenced right away #Tricon
Volkers: It was 'Munchausen syndrome by proxy' - (I had to look it up https://t.co/31He8xgOsA ) Nic is a $7M kid now. #Tricon
8:45pm February 20th 2017 via Hootsuite
Volker: Before the sequencing, they already reached their $2M maximum. Was under investigation for abuse (!) #Tricon
8:43pm February 20th 2017 via Hootsuite
Gallagher: Shares stories about struggles of the family; older daughters getting groceries; getting to know this family as people #Tricon
To get a sense of what this little Nic Volker went through, this photo at a #tricon panel https://t.co/w0o3nifvyZ
8:42pm February 20th 2017 via Hootsuite
Gallagher: "You have a story but without the family it's not much of a story". Their foundation: https://t.co/sgsJ4PxZtn #Tricon
8:38pm February 20th 2017 via Hootsuite
Worthey: Didn't get to the bottom of a list in a meeting; disbelief that they had found it. #Tricon
8:35pm February 20th 2017 via Hootsuite
Worthey: Asked at a CSHL meeting, and asked y/n questions via emails; none had found the same variant, neither any organism #Tricon
8:33pm February 20th 2017 via Hootsuite
Worthey: Had 12K variants, and 'ignore any variant above a frequency' but then there were no variant db's #Tricon
Gallagher: When we finally met Amylynn, we met a boy running around. Wanted to get the news out. #Tricon
8:30pm February 20th 2017 via Hootsuite
Gallagher: While speaking with a person, mentioned 'they sequenced all the genes of a patient', and thought there was a story here #Tricon
8:27pm February 20th 2017 via Hootsuite
Gallagher: Thought he was the youngest person to get Crohn's, failed all treatments. #Tricon
8:26pm February 20th 2017 via Hootsuite
Worthey: We couldn't use his name, so used 'sick kid' instead. Worked with 454, managed to get exome sequencing (this was '08) #Tricon
8:25pm February 20th 2017 via Hootsuite
Gallagher: There were detractors, who said that you would set the field back. #Tricon
8:23pm February 20th 2017 via Hootsuite
Worthey: Howard Jacob, found out about a boy who was about to die. So we said 'of course, we'll do whatever it takes to go faster' #Tricon
8:22pm February 20th 2017 via Hootsuite
Liz Worthey (HudsonAlpha AL) Could try to help kids in trouble - moved to WI to build up a team for genomic medicine #Tricon
8:21pm February 20th 2017 via Hootsuite
And now? Volker: He's normal, and hit a milestone - 'he hit puberty' #Tricon
8:20pm February 20th 2017 via Hootsuite
Co-author: Kathleen Gallagher 'he instead of sleeping with a teddy bear, would sleep with bagel bites that he couldn't eat' #tricon
8:19pm February 20th 2017 via Hootsuite
Volker: Many non-std treatments, wounds 'ripped open', couldn't eat, had IV-nutrition. #Tricon
8:18pm February 20th 2017 via Hootsuite
Volker: Slides show lots of pre- and post-photos. Colectomy to try and save his life; white cell transfusions, immune ablation #Tricon
8:17pm February 20th 2017 via Hootsuite
