Volker: His daily routine was to go to the hospital every day to clean his wounds. I 100 trips to the OR. Did hyperbaric trtmt #Tricon
8:16pm February 20th 2017 via Hootsuite
#Tricon keynote title "One in a Billion: the stoyr of Nic Volker and the dawn of genomic medicine" Amylynne Santiago Volker
8:15pm February 20th 2017 via Hootsuite
Panel #Tricon - Nic Volker's mother Amy Lyn https://t.co/5rhG9U8q0v Journalist here and book author. Also Liz Worthey
8:14pm February 20th 2017 via Hootsuite
Q:TAT for extraction of DNA/RNA? Conroy: 4-5d from specimen receipt, as they wait to batch. #Tricon
7:39pm February 20th 2017 via Hootsuite
Conroy: Mentions it took a lot of work to inform NY State with all the changes and data #Tricon
7:38pm February 20th 2017 via Hootsuite
Conroy: PyroMark, ABi 3500XL, SNVs and indels Ion PGM, Ion S5XL, Fusions, gene expresion MiSeq, CNV nCounter #Tricon
7:33pm February 20th 2017 via Hootsuite
Conroy: Able to extract 1x, go to RNA, and re-extract sample for DNA; shows nice yields of both. Validated across platforms #Tricon
7:32pm February 20th 2017 via Hootsuite
Conroy: Went from 12% QNS (quality not sufficient) to 4%. Now onto RNA extraction: showed table of yield differences, similar #Tricon
7:31pm February 20th 2017 via Hootsuite
Conroy: Showed panel of bioanalyzer traces of matched samples. NGS for mutations, Nanostring for CN analysis, 4/6 fail vs 6/6 pass #Tricon
7:29pm February 20th 2017 via Hootsuite
Conroy: Showed data side-by-side from matched cores, ave yield 1.5ug vs 4.6ug by Qubit cp Covaris vs 'Q' #Tricon
7:28pm February 20th 2017 via Hootsuite
Conroy: Shows acoustic lysis of the material. Regardless of mass, within 60 min ready to go into automated workflow (Covaris) #Tricon
7:26pm February 20th 2017 via Hootsuite
Conroy: How to extract? (organic, column, beads...) Manual or automated? How to deparaffinize? How and how long to lyse? #Tricon
7:23pm February 20th 2017 via Hootsuite
Conroy: Uses LabVantage LIMS to specimen data. For extractions - how many alternative? Liquid handling options? #Tricon
7:22pm February 20th 2017 via Hootsuite
Conroy: Use a tissue pick to scrape off material from 3-6 unstained slides. Also scrapers. Can multiply tubes. #Tricon
7:20pm February 20th 2017 via Hootsuite
Conroy: Needle core thru block to get high neoplastic nuclei and low stroma; then low neoplastic nuclei, high stroma but microenv #Tricon
7:19pm February 20th 2017 via Hootsuite
Conroy: Need DNA, RNA, max yield, HMW as possible, pure, low interferents, meet variety of downstream applications #Tricon
7:16pm February 20th 2017 via Hootsuite
Conroy: FFPE is crosslinked, can't control for age, storage, tumor mass, thickness, heterogeneity, histologies #Tricon
7:15pm February 20th 2017 via Hootsuite
Conroy: Challenge - going from specimen to complete reports. From needle cores, 20-40 blocks at any one time to a plate #Tricon
7:14pm February 20th 2017 via Hootsuite
Jeff Conroy (OmniSeq NY) Total nucleic acid profiling of FFPE tumors #Tricon
7:12pm February 20th 2017 via Hootsuite
Bly A2: A probiotic 'is like a drop in the ocean'. Single pills likely are not working. Need 20-30 pills to be effective for FMT #Tricon
7:08pm February 20th 2017 via Hootsuite
Q: When will you write a book? Bry: May be updating this '77 book https://t.co/Yv5tRYPC0r #Tricon
7:07pm February 20th 2017 via Hootsuite
Q; What is a 'normal FMT donor?' Bry: FMT is not going away. More defined consortia w/what pts actually need #Tricon
7:06pm February 20th 2017 via Hootsuite
Bry: Cancer: direct and idnirect; checkpoint inh is dependant on a primed immune system. Concludes - bugs as drugs #Tricon
7:05pm February 20th 2017 via Hootsuite
Bry: Even immune modulation by microbiota, including PD1 response. #Tricon
7:04pm February 20th 2017 via Hootsuite
Bry: Drugs are biotransformed: NSAIDS, irinotecan, sulfasalzine, digoxigenin - first 2 mediated toxicities, 2nd reduced efficacy #Tricon
Bry: Applications - biomarkers for dysbiotic conditions; ID of metabolites and biomarkers; push back to symbiotic commensalism #Tricon
7:02pm February 20th 2017 via Hootsuite
Bry: 'I don't want you to leave with the sense that the microbiome is still in the realm of vaporware' when it (clearly) is not. #Tricon
Bry: Able to go into defined cocktails w/commensals to reduce transmission of drug resistence, perhaps Rx interventions #Tricon
7:01pm February 20th 2017 via Hootsuite
Bry: For the C Diff model, organisms that affect C Diff either up or down. '16 Genome Biol ref https://t.co/cZEoRYyHkV #Tricon
6:58pm February 20th 2017 via Hootsuite
Bry: Did Bayesian projection of potential trajectories of infinite mixture model engine '12 PLOS ref https://t.co/WeLneW6WEH #Tricon
6:57pm February 20th 2017 via Hootsuite
Bry: Can a computational model be built to predict outcome? Put together putative protective communities, test in preclin model #Tricon
6:55pm February 20th 2017 via Hootsuite
Bry: Want to know essential composition; still need to go from donor to donor. Bringing together experimental, Pt and informatics #Tricon
6:54pm February 20th 2017 via Hootsuite
Bry: C diifficile, showing a yellow colon (with pus, yikes). First-line antibiotics, but FMT now std Rx recurrent. 80-90% effective #Tricon
6:53pm February 20th 2017 via Hootsuite
Bry: Now 'treat to cure': shows one spp was able to prevent IgE production. Human commensal bacteria: can it be done on people? #Tricon
6:52pm February 20th 2017 via Hootsuite
Bry: Showing IgE production against ovalbumin; small intestine mast cell recruitment shown, where anaphylaxis prevented (!) #Tricon
6:51pm February 20th 2017 via Hootsuite
Bry: Going to a Rx 'treat to prevent'; 8w period then followed by oral challenge, w/ an anaphylaxis temp drop. (Mouse model) #Tricon
6:50pm February 20th 2017 via Hootsuite
Bry: Gnoto-complex flora 44 human-culturable strains across 7 phyla, capitulating human microbiota by site, biochem and physiol fn #Tricon
6:49pm February 20th 2017 via Hootsuite
Bry: Foof sens., Th2 resp, IgE Ag, Mast cell recruit; re-exposure; allergic anaphylaxis. Looking at B-cell /IgA tolerance #Tricon
6:48pm February 20th 2017 via Hootsuite
Bry: ID'd Dx and Rx targets; admin. in mouse model; now looking at prospective clinical trials to prevent or cure food allergy #Tricon
6:47pm February 20th 2017 via Hootsuite
Bry: Environments may be too asceptic; doing 150 children longitudinal study; susceptible to food allergy, followed 3y #Tricon
6:46pm February 20th 2017 via Hootsuite
Bry: Food allergies cost $25B in lost time, illness. 6% children, 3% teens and adults. Epidemiology: caesarian reduced diversity #Tricon
Bry: Gnotobiotics, in vitro systems, synthetic biology. Going to clinical trials, and help direct what they will use. #Tricon
6:45pm February 20th 2017 via Hootsuite
Bry: Start w/large cohort w/clinical disease. Novel computational models (longitudinal, multiple variables, fn biology) #Tricon
6:44pm February 20th 2017 via Hootsuite
Bry: Rx could be prebiotics, probiotics, antibiotics, immunotherapeutics. Dietary changes, bugs as drugs, FMT #Tricon
6:43pm February 20th 2017 via Hootsuite
Bry: Also infection and immunity - dysbioses, susceptibility to infections (dysentery), pathobionts (IBD triggering flares) #Tricon
Bry: High sugar diet - lead to fermentation in-gut - potential cause of NASH nonalcoholic steatohepatitis #whatamouthful #Tricon
6:42pm February 20th 2017 via Hootsuite
Bry: Dental caries, to CV health, to digestive disorders. Functions - short chain fatty acids (sc-FA's). Ethanol production #Tricon
6:41pm February 20th 2017 via Hootsuite
Bry: Introduces with figure from '12 Nature https://t.co/RZ18x4tdvX Large chart of microbiota's involvement w/disease #Tricon
6:40pm February 20th 2017 via Hootsuite
Lynn Bry (Brigham & Women's Hosp MA) Precision microbiota applications in clinical therapy and diagnosis #Tricon
6:38pm February 20th 2017 via Hootsuite
Mata: Goal to generate testable hypotheses early; project teams need to engage support; need to engage regulatory, commercial teams #Tricon
6:37pm February 20th 2017 via Hootsuite
