Messing: NHGRI sequencing reduction, costs less and we're sequencing more. Conclude: the idea from 40y ago, from a free exchange #ASM2014
8:29am May 20th 2014 via Hootsuite
Messing: Reviews HGP was BAC by BAC; Venter's shotgun approach, photo of POTUS; then rice only gets 'Sec'y of Agriculture' #ASM2014
8:27am May 20th 2014 via Hootsuite
Messing: Paper by Zoller and Smith 1993 Nobel (Chemistry) 'tools and sequences for making chimeric genes' http://t.co/otUDejykC8 #ASM2014
8:25am May 20th 2014 via Hootsuite
Messing: So this paper in NAR (shotgun sequencing) becomes one of the most highly cited of all time: http://t.co/ezoGC78hii #ASM2014
8:23am May 20th 2014 via Hootsuite
Messing: Apple II sequencing software paper: http://t.co/Mt2J0jw5KN Concept for shotgun seq was rejected as 'trivial' by PNAS (!) #ASM2014
8:22am May 20th 2014 via Hootsuite
Messing: Reminds audience how difficult things like excising small fx and other molbio techniques were not that long ago. #ASM2014
8:20am May 20th 2014 via Hootsuite
RT @HWiatrowski: Joachim Messing is talking about phix174 at the Big Data session. Loving it - I'm such a sequencing history geek! #asm2014
8:16am May 20th 2014 via Hootsuite
Messing: Had to mutate F-factor to accel. conjugation, development of JM strains. Shows gel of lac repressor gene fragment #ASM2014
8:15am May 20th 2014 via Hootsuite
Messing: X-gal and lac operon, 1974 pub: http://t.co/QLiakDi9lb Tetramerization for active lactose, IPTG analogue... #ASM2014
8:13am May 20th 2014 via Hootsuite
Messing: (Note: I've worked with >so many< M13 clones, and now I'm hearing from the person who developed this! This is GREAT!) #ASM201
8:11am May 20th 2014 via Hootsuite
Messing: Idea of M13 ssDNA sequencing; M=Munich; unique to phiX due to lack of lysis. #ASM2014
8:10am May 20th 2014 via Hootsuite
Messing: Begins with this pub from his thesis in '72: http://t.co/mTczU7u1VI '74: parallel sequencing idea, after hearing Sanger #ASM2014
8:07am May 20th 2014 via Hootsuite
Joachim Messing (Rutgers Univ) (yes, >that< Messing): “Sequencing Long DNA Molecules and Modifying Them” #ASM2014 http://t.co/Q9RSGvr8
8:05am May 20th 2014 via Hootsuite
.@iontorrent PGM and 16S at #ASM2014 noon in the Product Theater http://t.co/57HnFOG9aF
7:36am May 20th 2014 via Hootsuite
RT @federicacocco: Independent article how the internet is changing journalism doesn't have a single link in it. :( http://t.co/LoTXKM1sc3
7:10am May 20th 2014 via Hootsuite
.@archaeal @kcwrighton Thanks for the reference!
5:59am May 20th 2014 via Hootsuite in reply to
.@drgitlin Yesterday with NASA folks at #ASM2014 I discovered there was a difference in stds for 'robotic' vs. 'human-weighted craft'
5:58am May 20th 2014 via Hootsuite in reply to
RT @kareynlo: Storified tweets #asm2014: Jo Handelsman & Graham Hatfull about SWI and SEA-PHAGES programs. http://t.co/lXbe5y0HeB
10:45pm May 19th 2014 via Hootsuite
RT @EricTopol: Excellent review of regulatory RNAs http://t.co/j3Frk062Bx @NatureRevGenet #genomics http://t.co/UWl4gwcdl3
9:50pm May 19th 2014 via Hootsuite
RT @ARKgenome: Dogs can detect prostate cancer with 98% accuracy: http://t.co/0J1a986Rkf Maybe this will help develop new #MDx.
8:25pm May 19th 2014 via Hootsuite in reply to
Reported at #ASM2014 @phylo_game: Microbial stowaways to Mars identified http://t.co/QMGS9hmIHl
6:58pm May 19th 2014 via Hootsuite
RT @scopedbylarry: CDC developed MERS diagnostic kits now dispatched to 41 countries around the world #asm2014
6:40pm May 19th 2014 via Hootsuite
.@kareynlo ...the first part of the session due to an overly-long conference call!
6:22pm May 19th 2014 via Hootsuite in reply to
.@kareynlo thanks for putting the Storify together. I regret missing
6:21pm May 19th 2014 via Hootsuite in reply to
Glaven: Fe oxidizing bact. for electron xfer at electrode: looking at Chromatiaceae, others #ASM2014
3:48pm May 19th 2014 via Hootsuite
Glaven: Only 16-17 genome clusters of the biofilm; publication in prep. #ASM2014
3:42pm May 19th 2014 via Hootsuite
Glaven: Naval research lab: proteomics work was most informative. Electrode biofilm was communicated via protein #ASM2014
3:39pm May 19th 2014 via Hootsuite
Glaven: Biofilm enriched 5y.o. from seawater; low diversity, stable, lives on electrode, CO2 and O2. 2013 http://t.co/lVRNBF30DN #ASM2014
3:37pm May 19th 2014 via Hootsuite
Glaven: A biocathode - single organisms live on the opposite side, drawing electrons out, microbes acting as catalysts #ASM2014
3:34pm May 19th 2014 via Hootsuite
Sarah Strycharz-Glaven: Electric Marine Biofilms For Bioenergy: Systems Approach To Drive Synthetic Biology Of Microbial Consortia #ASM2014
3:33pm May 19th 2014 via Hootsuite
Jin: Co-fermentation of cellobiose + xylose, presented figure from this 2011 PNAS http://t.co/0CKvi2GBvT #ASM2014
3:16pm May 19th 2014 via Hootsuite
Jin: If glucose + xylose together in fermentation, glucose preferred source. Described in 2009 PNAS http://t.co/HoLTrAHbiL #ASM2014
3:13pm May 19th 2014 via Hootsuite
Jin: Cloning 3 enzymes from Pichia to breakdown xylose; many iterations to increase yield PLOS One: http://t.co/b7kqzSHVjT #ASM2014
3:10pm May 19th 2014 via Hootsuite
Jin: S. cerevisiae: long history and many tools but can't use xylose; not suitable for cellulosic fermentation #ASM2014
3:07pm May 19th 2014 via Hootsuite
Jin: Sugar fermentation from corn glucose different than cellulosic biomass gives glucose, xylose + acetate, mixed ferment. #ASM2014
3:06pm May 19th 2014 via Hootsuite
Yong-su Jin (Univ IL at Urbana-Champaign): Synthetic Biological Widgets: Engineered Yeasts for Producing Fuels and Chemicals #ASM2014
3:02pm May 19th 2014 via Hootsuite
Bistué: Q: orientation matter for s10? A: Had to keep orientation colinear with the origin of replication to prevent collision #ASM2014
3:00pm May 19th 2014 via Hootsuite
Bistué: Of 4 isogenic strains, further away = slower replication (showed video evidence). Via qPCR: lower gene dosage #ASM2014
2:55pm May 19th 2014 via Hootsuite
Bistué: Project to change S10 location within V cholerae genome, even on secondary chromosome #ASM2014
2:52pm May 19th 2014 via Hootsuite
Bistué: V. cholerae a fast-grower, genes close to oriC1; ribosomal genes very close to s10 locus. Conserved universally #ASM2014
2:51pm May 19th 2014 via Hootsuite
Bistué: Obsv: fast-growing bact. have genes closer to replication origin, increasing gene dosage #ASM2014
2:50pm May 19th 2014 via Hootsuite
Bistué: Synthetic biology won't advance until genome organization / chromosome structure is better understood #ASM2014
2:49pm May 19th 2014 via Hootsuite
A. Soler-Bistué: s10-spec-α Repositioning in Vibrio cholerae Provides Insight into Genome Organization Rules of Fast-Growers #ASM2014
2:48pm May 19th 2014 via Hootsuite
RT @JesusAlvelo: Lots of great microbiology from across the globe at the #ASM2014. Loving the internationalization of the conference.
2:46pm May 19th 2014 via Hootsuite
Beisel: Used E.coli, promoter upstream of GFP, minimal M9 media, look at cells via flow cytometry. #ASM2014
2:36pm May 19th 2014 via Hootsuite
Beisel: Go back to the lac operon - positive feedback, an all-or-none response as a model of sugar utilization #ASM2014
2:34pm May 19th 2014 via Hootsuite
Chase L. Beisel: "Diverse Single-Cell Behaviors in Microbial Sugar Utilization" #ASM2014
2:33pm May 19th 2014 via Hootsuite
Handelsman: Bring microbiol. to students of all types; credits to Helmsley Charitable Trust, HHMI, Yale, and ASM #ASM2014
1:35pm May 19th 2014 via Hootsuite
Handelsman: Expanding: going int'l, also e-training this summer to scale. Next is chemical analysis; open access to materials #ASM2014
1:34pm May 19th 2014 via Hootsuite
Handelsman: Much higher completion rate in the SWI group with FL students #ASM2014
1:30pm May 19th 2014 via Hootsuite
