Handelsman: One example from State Coll. of FL: Eric Warrick did a shared lecture format, Trad. vs. SWI labs. Meas. improvement #ASM2014
1:29pm May 19th 2014 via Hootsuite
Handelsman: 900 students from Spring 2014; 4-7w compressed format; student posters ended up here at this meeting #ASM2014
1:28pm May 19th 2014 via Hootsuite
Handelsman: Spring 2014 26 institutions launched courses; variations on content to enrich protocols; tested tools / resources #ASM2014
1:27pm May 19th 2014 via Hootsuite
Handelsman: 6d 2013 Summer workshop, lab exp., pedagogical principles, design methods, courses to implement #ASM2014
1:26pm May 19th 2014 via Hootsuite
Handelsman: A Siberian student interacts with a Yale one. SWI pilot partners across the US map shown #ASM2014
1:25pm May 19th 2014 via Hootsuite
Handelsman: Small World Initiative (SWI): the potential to inspire to solve a real global problem. Engagement & network & interact #
1:24pm May 19th 2014 via Hootsuite
Handelsman: Choose an environmental sample, isolate and prioritize microbes, char organism, extract antibiotics, purify & char #ASM2014
1:23pm May 19th 2014 via Hootsuite
Handelsman: New habitats; small world is crowdsourcing discovery. Put the problem of antibiotic discovery, let freshmen solve it #ASM2014
1:22pm May 19th 2014 via Hootsuite
Handelsman: Antibiotic development: 75% come from soil bacteria; 100K natural products from Streptomyces spp #ASM2014
1:21pm May 19th 2014 via Hootsuite
Handelsman: MRSA: 64% mortality; 15x increase in mortality since 1993 #ASM2014
1:19pm May 19th 2014 via Hootsuite
Jo Handelsman (Yale): "The Small World Initiative: A Global Community of Undergraduate Researchers" #ASM2014
1:18pm May 19th 2014 via Hootsuite
RT @YinzMicrobes: Go to http://t.co/7cwgGoDn6O now for Fall 2015 SEA-PHAGES cohort applications #asm2014
1:16pm May 19th 2014 via Hootsuite
Hatfull: Annual symposium at HHMI Janelia Farm photo; application for Fall 2015 here http://t.co/wsyASFNxNC #ASM2014
1:15pm May 19th 2014 via Hootsuite
MT @shannonegreene: Hatfull: 2068 freshmen in SEA-PHAGE project with 6800 touched overall. Future @ASMicrobiology members! #asm2014
1:13pm May 19th 2014 via Hootsuite
RT @shannonegreene: Love it! Seeing phage names like "twister" and " barnyard." Science isn't all capital letters and numbers ;) #asm2014
A cool @BioFireDX ‘fire’ on the #ASM2014 exhibit hall. http://t.co/hGWgkuoSMl
12:21pm May 19th 2014 via Hootsuite
Wrighton: Instead of Fe reduction, a much different model for acetate; diagram in this ISME pub: http://t.co/Z0wDyM8ukE #ASM2014
9:55am May 19th 2014 via Hootsuite
Wrighton: ACD20 also observed in gut in this 2013 pub: http://t.co/wfJVE6y8UQ #ASM2014
9:53am May 19th 2014 via Hootsuite
Wrighton: aa auxotrophy, stable consortia, "I suspect many have a hydrogen economy" that requires a consortia of organisms #ASM2014
9:51am May 19th 2014 via Hootsuite
Wrighton: Looked at <0.2um samples that are 'division candidates'. Hypotheses: small size, G+-like walls, hydrogen economy #ASM2014
9:50am May 19th 2014 via Hootsuite
Wrighton: Pointed to this paper Nature 2013 that Tanya Woyke pointed to just an hour ago: http://t.co/n6dInwSZAC #ASM2014
9:48am May 19th 2014 via Hootsuite
Wrighton: 'Archaeal-like sulf-hydrogenase', still needs verification. #ASM2014
9:47am May 19th 2014 via Hootsuite
Wrighton: No cell wall, didn't find any sulfur, nor electron transport chain, expressing protein in ecosystem #ASM2014
9:46am May 19th 2014 via Hootsuite
Wrighton: 10 near-complete OD1 genomes; notable have small genome sizes (0.7 - 1.2Mb). Plot by GC. "No the cells aren't purple" #ASM2014
9:45am May 19th 2014 via Hootsuite
Wrighton: Presented the superphyla in this 2012 Science paper: http://t.co/7dMIEMmwII #ASM2014
9:43am May 19th 2014 via Hootsuite
Wrighton: Recovered 81 genomic bins, 74 bacteria, no archaea; 16S superphylum identified #ASM2014
9:42am May 19th 2014 via Hootsuite
Wrighton: Went to uranium ore mine tailing site, bioremediation effort (Old Rifle site) Geobacter spp, effect of acetate on system #ASM2014
9:40am May 19th 2014 via Hootsuite
Wrighton: (she's @kcwrighton ) Dark matter defined: what we know little about. Taxonomy fig Baker Dick 2013 http://t.co/0zRgyBKcVi #ASM2014
9:37am May 19th 2014 via Hootsuite
Kelly Wrighton (Ohio State): Illuminating Microbial Dark Matters: New Ecosystem Roles for Uncultivated Bacterial Phyla #ASM2014
9:33am May 19th 2014 via Hootsuite
Brady: Q: Efficiency of expression of clones? A: A majority, 50-60% with a standard promoter #ASM2014
9:32am May 19th 2014 via Hootsuite
@danudwary @bioinformer Interested in your reply. (Standing on my head didn't help.) :o)
9:31am May 19th 2014 via Hootsuite in reply to
Brady: eSNaPD software available online http://t.co/MDvus6C5lL Lab website: http://t.co/E6RhwxkAy1 #ASM2014
9:29am May 19th 2014 via Hootsuite
Brady: Shows Tetarimycin A, active against MRSA. Publication in J ACS 2013 here: http://t.co/cfXtZihmJ7 #ASM2014
9:27am May 19th 2014 via Hootsuite
Brady: Isolated aromatic polyketides: can show phylogeny and evolutionary ancestor. #ASM2014
9:26am May 19th 2014 via Hootsuite
.@danudwary Got to love rhetorical questions during presentations - the answer is usually yes...
9:25am May 19th 2014 via Hootsuite in reply to
Brady: Novel sequences: ~98% have no close relatives. Can we use a clade-based strategy instead? #ASM2014
9:23am May 19th 2014 via Hootsuite
Brady: Antracyclines (anti-cancer agent) can look for sequence tags in their libraries, sequence, express; assay (Arimetamycin A) #ASM2014
9:22am May 19th 2014 via Hootsuite
Brady: Is tracing chemical properties with geography, in the environment. Resistance mechanisms, promising leads #ASM2014
9:20am May 19th 2014 via Hootsuite
Brady: Some of this work appeared in his 2013 PNAS paper here: http://t.co/CAuvLwqHzH #ASM2014
9:17am May 19th 2014 via Hootsuite
Brady: Can go back to clone well to sequence the clone. 80% of the time the 400bp fragment works #ASM2014
9:14am May 19th 2014 via Hootsuite
Brady: First six samples: found 444 seq tags from TX soil, new erythromycin etc. Believable? 400bp from 100kb pathway? #ASM2014
9:13am May 19th 2014 via Hootsuite
Brady: Like with 16S - look for ketosynthase domains, adenylation domains #ASM2014
9:12am May 19th 2014 via Hootsuite
Brady: Natural product biosynth - only 6 major pathways with incredible diversity #ASM2014
9:11am May 19th 2014 via Hootsuite
Brady: Probe the sequences for novel chemistry: new glycopeptide biosynthetic pathways #ASM2014
9:09am May 19th 2014 via Hootsuite
Brady: Now: capture HMW DNA, make cosmids, 10M-20M clone 'mega libraries'. The phenotype screening also problematic #ASM2014
9:07am May 19th 2014 via Hootsuite
Brady: Goal is to find new natural products (small molecules). But starts with culture (only 1%), after culturing ~10-20% genes #ASM2014
9:06am May 19th 2014 via Hootsuite
Sean Brady (Rockefeller Univ): "Watch Where You Step, There Is New Chemistry Everywhere" #ASM2014
9:03am May 19th 2014 via Hootsuite
RT @microbegrrl: Rosenberg: “Sometimes stochastic only really means that we don’t know how it works” #asm2014
9:02am May 19th 2014 via Hootsuite
Hatzenpichler: Can also enrich for newly synth proteins: use alkyne-labeled biotin tags; affinity chromotography; prot detect #ASM2014
9:01am May 19th 2014 via Hootsuite
Hatzenpichler: Future directions include use of L-Homopropargyglycine (HPG) as another Met substitute; also sort based on activity #ASM2014
8:58am May 19th 2014 via Hootsuite
