LR: Autosomal STR, Y-STR, 'the police expect it'. report both results, YHRD freq, mixture calculation #HIDS15
7:55am March 11th 2015 via Twitter Web Client
LR: UK Nat'l crime figures: Sexual assault cases up 17% '12-'13. They employ 2 kits/case. Chart of 'compulsory dual workflow' #HIDS15
Lutz Roewer Universitatsmedizin Berlin: The Golden Age of forensic Y-STR typing #HIDS15
JB: More loci - perhaps with MPS - could get complete male differentiation #HIDS15
JB: 'If they have evidence and they don't test Y-STR they aren't doing their job' #HIDS15
JB: Recommend labs to provide 'or arrange to provide' Y-STR typing services. Should collect data up to 10d post. #HIDS15
JB: 4d sample 27/27 alleles; 9d 0/27. Conclude: QF Trio has 'excellent correlation' between male quant and profile recovery #HIDS15
7:54am March 11th 2015 via Twitter Web Client
JB: Nested data improved detection rate. But can't be readily performed due to primer mix. Next: need to eliminate pre-amp. #HIDS15
JB: Used nested-PCR flanking Yfiler primers to inc sensitivity. Graph of # alleles (out of 17), about 1/4 in the 15-17 range #HIDS15
JB: Now onto 4d, 7d and 9d samples - straight to Yfiler Plus & QF Trio. Non-diff extracts only. Not a typical validation (caution) #HIDS
JB: (In other words, non-sperm fractions had no profile using Yfiler Plus.) #HIDS15
JB: His 4/7 lined up exactly to expected Yfiler Plus results based on sample, QF ratio and DI number. 2/7 were the 3d samples #HIDS15
JB: Used QF Trio, got parameters from samples, M:F ratios ranged from 1:10K to 1:0.06. 4/7 profiles obtained via Yfiler #HIDS15
JB: Down to 31g male DNA (17 alleles). 1:4000 mixtures detect fine. Did 1, 2 and 3d profiles. Differential (organic) extraction #HIDS15
7:53am March 11th 2015 via Twitter Web Client
JB: Describes new Yfiler kits (info http://t.co/rSofpjQQlv ) more loci, more discrimination, better chemistry. Map of loci #HIDS15
JB: Shares illus from Pater 2005 ref http://t.co/AS0hIGS3zw with a decision threshold for CV disease. FP/FN and TP/TN rates #HIDS15
JB: Quantifiler Trio: can calculate M/F ratio. Can proceed to Y-STR analysis if needed. Product page: https://t.co/bVsJTtkn0T #HIDS15
JB: Quotes SWGDAM Y-STR paper here (PDF) http://t.co/XkBwZknMYp qPCR 'an important tool' for M/F quantitation #HIDS15
JB: He's been working on it since. Now only 1/2 labs in the US use Y-STR's. Y-STRs only found in males, not as discriminatory #HIDS15
JB: Yet literature has 7-10 post-intercourse sperm can be detected. 1998: the question posed: can Y-chr data be used? #HIDS15
JB: Std. STR profiling late-reported intimate post-coital samples. 24-48h: success lessens rapidly. >72h not normally possible #HIDS15
JB: No suspect ID'd, >72h. Some victims don't report >3d after incident - perhaps due to incapacitation. Policy to not accept >3d #
7:52am March 11th 2015 via Twitter Web Client
JB: Also - to prevent innocent individuals from being charged. Why some rape evidence not submitted? Not requested, backlog #HIDS15
JB: 'Every victim deserves the right to determine the genetic profile of their assailant!' #HIDS15
JB: In US, 60% of sexual assaults never reported; now 1/5 have been victims of attempted or completed rape in their lifetime #HIDS15
Jack Ballantyne Nat'l Ctr Forensic Science FL Why Y chromosome biomarkers are an essential tool in sexual assault investigations #HIDS15
CH: Dedicated to all the field workers - to save more animals from absolute cruelty and real risk of extinction #HIDS15
7:50am March 11th 2015 via Twitter Web Client
CH:Moving toward int'l cases: Vietnamese man jailed only 15mos Jan '14 for having 8 pieces of horn in transit in Singapore #HIDS15
CH: Training - make collection of evidence part of crime scene training. Provides much better-quality samples. #HIDS15
CH: Have put together chain of collection kits in collaboration with SA Police; eRhODIS electronic data collection for field-use #HIDS15
CH: Ref assisted chromosome assembly chart shown, using domestic horse as a reference. #HIDS15
7:49am March 11th 2015 via Twitter Web Client
CH: Routine analysis - blood, used 24 STRs, improved markers under development. @appliedbio helping development for int'l use #HIDS15
CH: Middle of horn shown to be best for DNA. Can match blood ("Bluestar Forensic Latent blood reagent") from evidence #HIDS15
CH: Person who had $450K suspected of transport of horn - able to obtain two DNA matches, tied back to illegal poaching #HIDS15
CH: Published extraction protocol in '13 http://t.co/d2TWol8Fqs Extracted from 1888 sample also 1938 one #HIDS15
CH: DNA extraction - horn is an epitermal derivative. Can be harvested from live animal, no bony core, 3y to regrow. #HIDS15
CH: Rhino horn jewelry 'widely available if you know where to look for them'. #HIDS15
CH: Used as a status symbol of consumer countries: Vietnam and China. Like a Ferrari - an expensive imported item, used w/alcohol #HIDS15
CH: Rhino horn a traditional Chinese medicine; now a rumored cancer cure in Vietnam. A 'party drug and status symbol' #HIDS15
CH: Rhino horn described as 'like cocaine, minus the risk'. Most valuable illegally traded commodity. #HIDS15
7:48am March 11th 2015 via Twitter Web Client
CH: Charted poaching arrests, 165 in '10; 344 in '14. From poacher to intermediary to national buyer to int'l exporter #HIDS15
CH: Poaching numbers: 13 in '07, 333 in '10, 1215 in '14. "Out of control now." "Decline exceeding reproduction rate." #HIDS15
CH: 'Our freezers are constantly full'. Photo of dead animal, saws and axes, horn matched to individual carcass at crime scene #HIDS15
CH: MOU with Kenya Wildlife too. Forensic cases: 22 in '10 to 1067 in '14. Done with very limited funding in animal forensics #HIDS15
CH: Total number (alive and dead) in RhODIS: 15K. From SA, state and private owners (5K0, many other African countries #HIDS15
CH: Only 5 Northern White Rhinos left; 18K Southern White Rhinos. 98% in South Africa #HIDS15
Cindy Harper Veterinary Genetics Laboratory Univ Pretoria S Africa The Rhinoceros DNA database: a forensic tool #HIDS15
Special session: How Rhino DNA database can help save a species from extinction http://t.co/0Ug3kpRw5W
7:47am March 11th 2015 via Twitter Web Client
TS: Believes pressure once NGS in-place - to expand autosomal, tDNA, more information in db's, not less #HIDS15
TS: As precision medicine has so much more data and impact - health, gov't agency, insurance. While for forensics limited ID #HIDS15
