Bernards: #ampeurope2018 Hi-dose chemo, high cross-linking, same effect as PARPi that needs HEJR mechanism.
3:10am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 'For AZ, a billion dollar question'. (!) 1/3 w/promoter methylation, unexpressed.
3:09am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 Doing aCGH, a spec pattern of CN gain/loss when cp to sporatic TN cancer. Can find 3x CN pattern but BRCA1 wt
3:08am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 Bet 6%-20% of TNBC are germline BRCA1 mut, sense to PARPi. Is there a BRCA1-like signature… https://t.co/59FhPe1qnq
3:07am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 CD4, CD8 higher in IR subtype, alogn with higher PD1, PD-L1, CTLA4. Thus PD1/L1/CTLA4 inh… https://t.co/GUDh6FJlYQ
3:05am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 Two intrinsic subtypes of lobular br ca: chemo not very effective. Via GEx, two groups: Im… https://t.co/Krjbs9wM3y
3:03am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 Shows this PD-1 blockade with MMR https://t.co/aWXuwqo4hy and shows this: MSI-like, for CRC https://t.co/FbgL7kTii8
3:01am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 'Mutation analysis is not the be-all, end-all for personalized medicine'. 'And I'll continue beating this horse...'
2:59am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 Shows K-M of BRAF-like colon ca pts, w/poor outcome but sens to vinorelbine as possible Rx strategy.
2:58am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 Some colon tumors have the gene sig of BRAF mutant tumors, but lack BRAF mut. https://t.co/2N893BdlQa
2:56am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 And EGFRi not useful for melanoma, b/c not expressed. A single gene expressed making the response different
2:54am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 Shows effectiveness in PDX mice, and marked clinical response in human. PhIII BEACON study… https://t.co/hYbNGoF4FX
2:53am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 Finds 'massively interesting as a scientist'. Synthetic lethality screen: had to inhibit EGFR too along w/BRAF.
2:52am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 And MEK inh, only 1/2 w/BRAFi, V600E not limited to melanoma. CRC: Vemurafenib not useful… https://t.co/Z63vPjVIxG
2:50am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 Shows famous Wagle photo: 'the poster child of personalized medicine'. BRAF mutant first response very good...
2:49am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 Reverting EMT via chemoRx response of TGF-beta. MammaPrint: 70 gene sig, back from '02 to assess risk in br ca
2:47am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 Makes the case for Br, CRC, GB, Panc subtypes 'cannot be identified through DNA analyses only'
2:46am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 The need to analyze RNA; CRC has 3-5 intrinsic subtypes; shows 3 via gene expression and K-M curves
2:44am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 'We like to complain in Holland in general.' Commentary: colon cancer subtypes have different biology.
2:42am May 1st 2018 via Twitter Web Client
Bernards: #ampeurope2018 'A case of Irrational Exuberance?' '16 ref https://t.co/LDKyUTrMwC And Vinay Prasad soon after, NEJM Tannock
Bernards: #ampeurope2018 Changing views of NSCLC: from a morphology-centric to a genome-centric view. Shows long list of genes/drugs
2:40am May 1st 2018 via Twitter Web Client
Rene Bernards (Netherlands Cancer Inst, The Netherlands) #ampeurope2018 Next Gen Pathology Molecular Dx: Combining… https://t.co/KSTVRAuZc3
2:39am May 1st 2018 via Twitter Web Client
Ahadova: #ampeurope2018 (Here's the ASCO 2015 PDF of the Lynch Syndrome FSP vaccine from Oryx https://t.co/G5BY1biUlc )
2:36am May 1st 2018 via Twitter Web Client
Ahadova: #ampeurope2018 Have developed a FSP vaccine - ASCO 15 results https://t.co/8nVsJ5r2yG
2:32am May 1st 2018 via Twitter Web Client
Ahadova: #ampeurope2018 Looked at spec KRAS in MMR-def cells; vast majority occurs after MMR deficiency in KRAS. Fo… https://t.co/BcVzDxRJN4
2:21am May 1st 2018 via Twitter Web Client
Ahadova: #ampeurope2018 Three molecular pathways model '18 ref https://t.co/aBgjJkyHhe for CRC in Lynch
2:20am May 1st 2018 via Twitter Web Client
Ahadova: #ampeurope2018 Asking when MMR- deficiency a late event; correlatoin of MMR w/size, grade of adenomas.
2:18am May 1st 2018 via Twitter Web Client
Ahadova: #ampeurope2018 Colonoscopy does not prevent all colon cancer in Lynch; 15% risk in 10y. https://t.co/K8jLvz9UzU
2:17am May 1st 2018 via Twitter Web Client
Ahadova: #ampeurope2018 Warthin's seamstress, published a family tree in 1913. Cancer risk for Lynch - penetrance not so high.
2:14am May 1st 2018 via Twitter Web Client
Ahadova: #ampeurope2018 15% of CRC is MSI-H; of that 3/4 is sporadic, MLH1 promoter methylated. Knudson's two-hit theory w/Lynch Syndrome
2:12am May 1st 2018 via Twitter Web Client
Ahadova: #ampeurope2018 Mutational load from '13 https://t.co/CXv7Pq01rJ Rationale why MSI-H is a readout for sporadic CRC
2:11am May 1st 2018 via Twitter Web Client
Ahadova: #ampeurope2018 Frameshifted peptides (FSP) are new to the immune system. Shows image from https://t.co/1nOYblvbOi
2:10am May 1st 2018 via Twitter Web Client
Ahadova: #ampeurope2018 MSI tumor evolution: recurr mutation that leads to tumorigenesis in a tissue-spec way.
2:08am May 1st 2018 via Twitter Web Client
Ahadova: #ampeurope2018 Illustrates repetitive homopolymers, and how polymerases can lead to single base indels, an… https://t.co/DuYoxp8qkB
2:06am May 1st 2018 via Twitter Web Client
Aysel Ahadova (Heidelberg, Germany): #ampeurope2018 New Insights in the Pathogenesis of MSI Cancers
2:05am May 1st 2018 via Twitter Web Client
Q: Upper limit of multiplexing? Abudayyeh: #ampeurope2018 Now single-plex; 4-plex underway; Ag-Ab interaction dep, likely 10plex
12:09pm April 30th 2018 via Twitter Web Client
Q: What about commercialization? Abudayyeh: #ampeurope2018 1y conversations about how best to do this, inf disease, Ag, Gates Found
12:07pm April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 RNAi doesn't work well in nucleus, but their system works well there to understand mechanism
12:06pm April 30th 2018 via Twitter Web Client
Q: Exploiting lincRNA at all? Abudayyeh: #ampeurope2018 Cas13d new protein for modulating splicing; may expand SHERLOCK.
12:05pm April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 RNA editing - targeting, converting specific A to I. https://t.co/mWY6K3uPdL Showing off-… https://t.co/Ax9jQvuG6E
12:00pm April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 For RNA therapeutics: how to target in the cell? Shows Cas13a K-D more spec than RNAi. '1… https://t.co/rDgGiJYrLM
11:56am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Lateral flow is exciting as it is field-deployable. Pushing technology with more applications, variants.
11:53am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Thinking that six-plex is feasible for fluorescence; up to 10-plex on lateral flow. Working on sepsis.
11:52am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Shows results in lateral flow strips. Also showed EGFR ex 19 del. Published in Gootenberg et al '18
Abudayyeh: #ampeurope2018 Onto liquid biopsy with lateral flow: Brigham and Women's collab. RPA 30', LwaCas13a reaction, look for EGFR L858R
11:51am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Single-base Zika mutation det w/lateral flow. N139S assoc'd with microencephaly in Zika, showed results
11:50am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 (Distracted thanks to misspelling of author's name Myhrvold et al in this week's Science)
11:49am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Two application to human samples: Zika, 40 South Am samples. https://t.co/ZY2FWzjb5G
Abudayyeh: #ampeurope2018 Boosting signal via cyclic activator '17 https://t.co/mlQftW4AXH Cas13 + Csm6 RNAses
11:43am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Shows [aM] sens after 1h. 'Likely working down to single molecule'
11:42am April 30th 2018 via Twitter Web Client
