Abudayyeh: #ampeurope2018 In lateral flow strip Strepavidin strip capture, if FAM is attached or separated - at the second Ab line readout
11:41am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Portability w/a visual readout? Current method needs a fluoresent plate-reader. Used FAM + Biotin (FAM for Ag det)
11:40am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Getting quantitative: dilution series, optimized primer conc w/real-time readout.
11:39am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Explains multiplexing method - ortholog base preference motifs. Shows 4plex data.
Abudayyeh: #ampeurope2018 Detecting mutations - most recent work w/multiplexing, quant, visual readout, inc signal… https://t.co/LBSFYLaKLg
11:36am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Tried freeze-drying reagents on paper-based Dx; 95C 2' for thermal lysis, 37C 20' RPA. Also [aM] sens
11:34am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 SHERLOCK - tested against Zika, from blood or urine. Shown to 1.25 cp/uL Science '17 ref
11:33am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Describes SHERLOCK - spec high sen enzymatic reporter unlocking. 1 buffer 2 aM sens https://t.co/mWY6K3uPdL
11:32am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Collateral RNA: w/a fluor and quencher, a sensing mechanism https://t.co/tQnnYyvZ0m
11:31am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Rapid potential for Dx NEJM '17 https://t.co/naaW0XrAYf
11:30am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Phage RNA, then any other nearby RNA. Useful for 'detection of any nucleic acid'.
11:29am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Tackling the RNA-guided RNAse, the RNA infection immune system. Used as Dx: binds to ssRN… https://t.co/xBm45wI8cm
Abudayyeh: #ampeurope2018 New effectors - described here https://t.co/2mlqFEfEGy C2c2 now called Cas13a '16 Science https://t.co/h9MopgmClt
11:28am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Updated Class II ontology https://t.co/byuxNcNgGo Expanding simpler Class II - using sign… https://t.co/jYEV1jCO46
11:25am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Classifications https://t.co/30hZRzKWpn New ones: Class I has many genes vs Class II (Cas9, only 1 gene)
11:24am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Cas9 is the first; many other types of CRISPR systems '14 rev https://t.co/TIg4gQeKKe Emerging CRISPR dx
11:22am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Other areas - viral vectors, optogenetics, and gene editing CRISPR in particular.
11:20am April 30th 2018 via Twitter Web Client
Abudayyeh: #ampeurope2018 Comes from Feng Zhang's group at the Broad. Novel biology exists in nature: From restrict… https://t.co/aNtjTBNiaK
Omar Abudayyeh (Broad Inst MA) #ampeurope2018 Harnessing novel CRISPR systems for transcriptome engineering and human health
11:19am April 30th 2018 via Twitter Web Client
Q: Molecules vs sequenced molecules at 100%? Deitz: #ampeurope2018 Surprising, but parameters may have bias (i.e. i… https://t.co/eZF3jg0YHV
10:27am April 30th 2018 via Twitter Web Client
Deitz: #ampeurope2018 Technical sens still a limitation; ctDNA fx and total amount will limit detection. Avenio tar… https://t.co/HUpX6JfrrS
10:23am April 30th 2018 via Twitter Web Client
Deitz: #ampeurope2018 Shows GEq vs unique coverage by input amount; nice correlation.
10:18am April 30th 2018 via Twitter Web Client
Deitz: #ampeurope2018 TKI T780M is 40-55%; T790M w/ampl is another 10%. Copies/mL plasma.
10:15am April 30th 2018 via Twitter Web Client
Deitz: #ampeurope2018 66 ng needs many mLs of plasma. Dynamic monitoring. 15/16 pts mutations det '16 https://t.co/uLF64mmIn4
10:10am April 30th 2018 via Twitter Web Client
Deitz: #ampeurope2018 Chart of tumor derived DNA; if 0.1%, need 1K GEq, 6.6ng. 0.01%, need 10K GEq, 66ng. 0.001%, 100K GEq, 660ng
10:09am April 30th 2018 via Twitter Web Client
Deitz: #ampeurope2018 Nice chart of sens, method, info, cost TAT from https://t.co/V3IhiWuOTK
10:08am April 30th 2018 via Twitter Web Client
Deitz: #ampeurope2018 Shows usefulness of early det, profile, MRD, Rx resp, Rx resis, ID of lethal clones 'a molecular autopsy'
10:07am April 30th 2018 via Twitter Web Client
Deitz: #ampeurope2018 Shows tissue vs liquid biopsy: minus of tissue is invasiveness, tumor het, clonal evolution.… https://t.co/T8z2Bh3e9u
10:05am April 30th 2018 via Twitter Web Client
Steffan Deitz (DKFZ): #ampeurope2018 Application of ctDNA in the context of NSCLC
10:04am April 30th 2018 via Twitter Web Client
Palma: #ampeurope2018 HR of 27.8 among TTR (time to recur), if ctDNA positive. Replapse-free survival. Irrespective… https://t.co/KsRMgr8Wi2
10:02am April 30th 2018 via Twitter Web Client
Palma: #ampeurope2018 Onto surveillance for CRC stages II/III; ID SV's from tissue. n=145, 144/145 ID'd variants. 1… https://t.co/cgqNY2ZHGY
9:59am April 30th 2018 via Twitter Web Client
Palma: #ampeurope2018 Shows STEAM study Hurwitz ASCO GI '17 result (manuscript in prep) n=280 pre-induction for CRC… https://t.co/cEDdiqnac5
9:52am April 30th 2018 via Twitter Web Client
Palma: #ampeurope2018 Shows K-M fig from paper, survival the same even if mutant KRAS.
9:50am April 30th 2018 via Twitter Web Client
Palma: #ampeurope2018 n=92 in mCRC, CAPRI-GOIM w/matched tissue/plasma BEAMing w/Plasma. https://t.co/hQsIwVjQ7G 70… https://t.co/lDFleAP1Kb
9:48am April 30th 2018 via Twitter Web Client
Palma: #ampeurope2018 Detect and track molecular changes: understanding the patient journey. Fig from '17 https://t.co/o6n2IjClCU
9:47am April 30th 2018 via Twitter Web Client
Palma: #ampeurope2018 Voices: reimbursement landscape is rough. "A lot of the pain points are still yet up-front" (… https://t.co/peNNGGGsAA
9:45am April 30th 2018 via Twitter Web Client
Palma: #ampeurope2018 Precision med today: genetic research and testing is the gateway to its promise; NGS is all about results in time.
9:44am April 30th 2018 via Twitter Web Client
Palma: #ampeurope2018 Shows K-M curves from publ. like this one https://t.co/FFnXbKRmqh of NSCLC and effectiveness
9:42am April 30th 2018 via Twitter Web Client
John Palma (Roche): #ampeurope2018 Clinical research applications in CRC. Will have more data at upcoming #ASCO18
9:41am April 30th 2018 via Twitter Web Client
Droege: #ampeurope2018 Oct '18 for tumor tissue with same genes for matched tissue / ctDNA analysis. 5-day workflow, 'end-to-end'
9:40am April 30th 2018 via Twitter Web Client
Droege: #ampeurope2018 SW does clustering and database integration w/COSMIC, TCGA db's.
9:39am April 30th 2018 via Twitter Web Client
Droege: #ampeurope2018 Soes very good sens at 20M reads at 0.5% AF across 3 products, 30ng or 50ng inputs
9:38am April 30th 2018 via Twitter Web Client
Great to find at #ampeurope2018 the first https://t.co/ugGjrmD6Mf commercial presence - real-time PCR and NGS for clinical, research markets
9:37am April 30th 2018 via Twitter Web Client
Droege: #ampeurope2018 Expanded: for progression; targeted for profiling pre-Rx. Shows integrated Digital Error Suppression (iDES)
9:36am April 30th 2018 via Twitter Web Client
Droege: #ampeurope2018 3 panels: Targeted 17 genes 81kb; Expanded 17 + 60 = 77 192kb; Surveillance 180+17 = 197 genes
Droege: #ampeurope2018 Optimized for NextSeq 500; coming up with analysis pipeline, spec report.
9:35am April 30th 2018 via Twitter Web Client
Droege: #ampeurope2018 Intended RUO, 4mL plasma, research subjects lung, CRC. All 4 mut classes in one panel; can do 16-plex on ILMN
9:34am April 30th 2018 via Twitter Web Client
Droege: #ampeurope2018 Limited input molecules in ctDNA; background reduction (they use error suppression), coverage - they have 3 designs
9:33am April 30th 2018 via Twitter Web Client
Marcus Droege (Roche) Technical overview of Avenio ctDNA analysis RUO #ampeurope2018
9:32am April 30th 2018 via Twitter Web Client
Greenawalt: #ampeurope2018 ID'd 4/40 JAK1 LoF muts w/previously-treated pts.
8:50am April 30th 2018 via Twitter Web Client
